Reviewed, UniProtKB/Swiss-Prot O52703 (T2A1_ACEPA)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme ApaLI
      Short name=R.ApaLI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme ApaLI
    Endonuclease ApaLI

Gene names

Name:

apaLIR

Organism

Acetobacter pasteurianus (Acetobacter turbidans)

Taxonomic identifier

438 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acetobacter

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GTGCAC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Type-2 restriction enzyme ApaLI		PRO_0000077275

Sequences

	Sequence		Length	Mass (Da)
	O52703-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9E4FFBB05279A94C	FASTA	375	41,730
	10 20 30 40 50 60 MTTRQRLSAE RSQQLTRLLT ITKTANMRAL MEASELAKVI ALVAVDIGKS DEMARAFPVL 70 80 90 100 110 120 WPKISPQQEY YATAVDWFTN PDETVTSFDV VDMLDAGTSL DQDFMTYLKC LTELHKRRRK 130 140 150 160 170 180 YGLILQRQPL PTMVQVSPRA LMEYGPDFPP EALASWLTWR KFFYDLDNRS AQETGYLFEP 190 200 210 220 230 240 ILAAAIGGEA KSARERVVRR TDDPTKGRQV DCWKVLPDGT PLAYELKLRV TIAASGQGRF 250 260 270 280 290 300 GEELSFARDC SSSGAKPILV VLDPTENDKL TGLQAAYREV GGAAYVGDAA WAHLEDEAGA 310 320 330 340 350 360 TMASFIERYV RVPVASVSSF ERVIEGDATK RSLILQDLQA RLDGNELTIS LGGHQRLVER 370 HEDQSLAADG DDDSE

References

[1]

"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli." Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998) [PubMed: 9862476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 12875.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF044847 Genomic DNA. Translation: AAC97181.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	85. ApaLI.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2A1_ACEPA

Accession

Primary (citable) accession number: O52703

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 1998
Last modified:	November 4, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)