Reviewed, UniProtKB/Swiss-Prot O61125 (STK4_DICDI)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase 4 homolog A
    EC=2.7.11.1
Alternative name(s):
    STE20-like kinase krsA
    Kinase responsive to stress A

Gene names

Name:	krsA
Synonyms:	krs1
ORF Names:	DDB_0191170

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulates both cAMP signaling during early development and the stress response. Functions as an activator of adenylylcyclase.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Manganese.
Subcellular location	Cytoplasm › cytosol.
Developmental stage	Expressed specifically at aggregation-stage.
Post-translational modification	Undergoes autophosphorylation in the catalytic domain.
Miscellaneous	Mutants lacking krsA exhibit reduced viability under hyperosmotic conditions. They produce smaller aggregates on membrane filters and do not form aggregation streams on a plastic surface under submerged starvation conditions, but are normal in sexual development. During early asexual development, the expression of cAMP related genes peaks earlier in the knockout mutants. Neither cAMP oscillation in starved cells nor an increase in the cAMP level following osmotic stress was observed in mutants lacking krsA.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. Contains 1 SARAH domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 461	461	Serine/threonine-protein kinase 4 homolog A
Regions
	Domain	20 – 273	254	Protein kinase
	Domain	411 – 458	48	SARAH
	Nucleotide binding	26 – 34	9	ATP By similarity
	Compositional bias	309 – 316	8	Poly-Glu
Sites
	Active site	139	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Amino acid modifications
	Modified residue	173	1	Phosphothreonine; by autocatalysis By similarity
Experimental info
	Mutagenesis	49	1	K → R: Do not affect the function
	Mutagenesis	173	1	T → A: Stream formation negligible

Sequences

	Sequence		Length	Mass (Da)
	O61125-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 947547565827B9EE	FASTA	461	52,453
	10 20 30 40 50 60 MSTLNVPKET MSRKDPEKFF TIVEKLGEGS YGSVYKAINI STGIVVAIKK VSVDNDLEDM 70 80 90 100 110 120 EKEISFMKQC KSPYIVTYYA SFRKENEVWI VMEHCGAGSV CDAMKITDKT LSEDQIAVVS 130 140 150 160 170 180 RDVLQGLAYL HSVRKIHRDI KAGNILMNHK GESKLADFGV SGQLSDTMAK RQTVIGTPFW 190 200 210 220 230 240 MAPEVIQEIG YDYKADIWSY GITCIEMAES KPPLFNVHPM RVIFMIPNPS RPPPKLTEPE 250 260 270 280 290 300 KWSPEFNDFL AKCLTRKPEL RPSAEELLKH PFITKAKSHS LLVPLIDEQD IIINEKGREV 310 320 330 340 350 360 ALGIEQRDEE EEDEDEDSED SDDNRGTMVR AKPRSMQNSG GEDNDEEYDT GTMVITDNKN 370 380 390 400 410 420 SYDTVVFNND DEDSGTMKLK NTMPSNKKNF VPDYMNQFKK SDDDVTNVPL SDKYSSYSLE 430 440 450 460 ELKKMLAELE IEREKEVQKT LEKFSINRQA LLAVIDEKKS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Ste20-like kinase Krs1 of Dictyostelium discoideum." Arasada R., Son H., Ramalingam N., Eichinger L., Schleicher M., Rohlfs M. Eur. J. Cell Biol. 85:1059-1068(2006) [PubMed: 16842885] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, FUNCTION, COFACTOR, AUTOPHOSPHORYLATION, SUBCELLULAR LOCATION. Strain: AX3-1.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"A stress response kinase, KrsA, controls cAMP relay during the early development of Dictyostelium discoideum." Muramoto T., Kuwayama H., Kobayashi K., Urushihara H. Dev. Biol. 305:77-89(2007) [PubMed: 17362909] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-49 AND THR-173, KNOCKOUT. Strain: AX3-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF061281 mRNA. Translation: AAC15972.1. AAFI02000064 Genomic DNA. Translation: EAL65279.1.
RefSeq	XP_638650.1.
3D structure databases
HSSP	HSSP built from PDB template 1PXI based on UniProtKB P24941.
ModBase	Search...
Genome annotation databases
GeneID	3390225.
KEGG	ddi:DDB_0191170.
Organism-specific databases
dictyBase	DDB0191170. krsA.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR011524. SARAH. IPR017442. Se/Thr_pkinase-rel. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS50951. SARAH. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

STK4_DICDI

Accession

Primary (citable) accession number: O61125
Secondary accession number(s): Q54PZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	August 1, 1998
Last modified:	September 23, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)