Reviewed, UniProtKB/Swiss-Prot O61397 (GALT7_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable N-acetylgalactosaminyltransferase 7
    EC=2.4.1.-
Alternative name(s):
    Protein-UDP acetylgalactosaminyltransferase 7
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7
      Short name=pp-GaNTase 7

Gene names

Name:	gly-7
ORF Names:	Y46H3A.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable glycopeptide transferase involved in O-linked oligosaccharide biosynthesis. Glycopeptide transferases catalyze the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide By similarity. In contrast to other members of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on peptides that have been tested. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane proteinBy similarity.
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar binding Inferred from electronic annotation. Source: UniProtKB-KW transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Probable N-acetylgalactosaminyltransferase 7		PRO_0000059150
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 38	18	Signal-anchor for type II membrane protein Potential
	Topological domain	39 – 601	563	Lumenal Potential
	Domain	477 – 595	119	Ricin B-type lectin
	Region	155 – 265	111	Catalytic subdomain A
	Region	328 – 390	63	Catalytic subdomain B
Amino acid modifications
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Disulfide bond	490 ↔ 506		By similarity
	Disulfide bond	529 ↔ 542		By similarity
	Disulfide bond	568 ↔ 583		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O61397-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 1A7E744C93BDCDE7	FASTA	601	68,108
	10 20 30 40 50 60 MIIARKKLQL QRLWRQRGCR VATYICLGVL VLFGFVYNSK GNSMSSIKSD SAAQQFDDLD 70 80 90 100 110 120 DLTNKELPGG PDPNTIFRGS ELGNYEPKEP EIPSNQPGEH GKPVPVTDEE GMAAGRAAEK 130 140 150 160 170 180 EFGFNTYVSD MISMNRTIPD IRPEECKHWD YPEKLPTVSV VVVFHNEGWT PLLRTVHSVL 190 200 210 220 230 240 LRSPPELIEQ VVMVDDDSDK PHLKEKLDKY VTRFNGKVIV VRTEQREGLI NARSIGAKHS 250 260 270 280 290 300 TGEVVLFLDA HCEVNTNWLP PLLAPIKRNR KVMTVPVIDG IDSNSWEYRS VYGSPNAHHS 310 320 330 340 350 360 GIFEWGLLYK ETQITERETA HRKHNSQPFR SPTHAGGLFA INRLWFKELG YYDEGLQIWG 370 380 390 400 410 420 GEQYELSFKI WQCGGGIVFV PCSHVGHVYR SHMPYSFGKF SGKPVISINM MRVVKTWMDD 430 440 450 460 470 480 YSKYYLTREP QATNVNPGDI SAQLALRDKL QCKSFKWYME NVAYDVLKSY PMLPPNDVWG 490 500 510 520 530 540 EARNPATGKC LDRMGGIPGP MGATGCHGYG GNQLIRLNVQ GQMAQGEWCL TANGIRIQAN 550 560 570 580 590 600 HCVKGTVNGF WSYDRKTKQI IHSQKRQCIT VSESGSEVTL QTCTEDNERQ KFVWKEFYQS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans." Hagen F.K., Nehrke K. J. Biol. Chem. 273:8268-8277(1998) [PubMed: 9525933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF031841 mRNA. Translation: AAC13677.1. AC006774 Genomic DNA. Translation: AAF60620.1.
PIR	T42251.
RefSeq	NP_503512.1.
UniGene	Cel.19664
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	Y46H3A.6. Caenorhabditis elegans. [Contig view]
GeneID	178661.
KEGG	cel:Y46H3A.6.
Organism-specific databases
WormBase	WBGene00001632. gly-7.
WormPep	Y46H3A.6. CE24309. [WorfDB]
Gene expression databases
ArrayExpress	O61397.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
NextBio	902042.

Entry information

Entry name

GALT7_CAEEL

Accession

Primary (citable) accession number: O61397

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 1, 1998
Last modified:	November 4, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project