Reviewed, UniProtKB/Swiss-Prot O61491 (FLOT1_DROME)

Names and origin

Protein names

Recommended name:
    Flotillin-1

Gene names

Name:	Flo
Synonyms:	FLODM-1
ORF Names:	CG8200

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Subunit structure	Heterooligomeric complex of flotillins 1 and 2 and caveolins 1 and 2 By similarity.
Subcellular location	Cell membrane › caveola; Peripheral membrane protein. Note= Membrane-associated protein of caveolae.
Tissue specificity	Expressed in brain and ventral nerve cord from stage 12-16 of embryogenesis.
Sequence similarities	Belongs to the band 7/mec-2 family. Flotillin subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	flotillin complex Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural molecule activity Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9W1K5-1	1	EBI-212825,EBI-212772

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Short (identifier: O61491-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Long (identifier: O61491-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-162: G → GDSKG

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 426	426	Flotillin-1
Natural variations
	Alternative sequence	162	1	G → GDSKG in isoform Long.

Sequences

	Sequence		Length	Mass (Da)
	Isoform Short [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 64C99F9CB4676C0C	FASTA	426	47,135
	10 20 30 40 50 60 MTWGFVTCGP NEALVVSGCC YMKPLLVPGG RAFVWPVGQQ VQRISLNTMT LQVESPCVYT 70 80 90 100 110 120 SQGVPISVTG IAQVKVQGQN EDMLLTACEQ FLGKSEAEIN HIALVTLEGH QRAIMGSMTV 130 140 150 160 170 180 EEIYKDRKKF SKQVFEVASS DLANMGITVV SYTIKDLRDE EGYLRSLGMA RTAEVKRDAR 190 200 210 220 230 240 IGEAEARAEA HIKEAIAEEQ RMAARFLNDT DIAKAQRDFE LKKAAYDVEV QTKKAEAEMA 250 260 270 280 290 300 YELQAAKTKQ RIKEEQMQVK VIERTQEIAV QEQEIMRRER ELEATIRRPA EAEKFRMEKL 310 320 330 340 350 360 AEANKQRVVM EAEAEAESIR IRGEAEAFAI AAKAKAEAEQ MAMKAEAYRE YREAAMVEML 370 380 390 400 410 420 LDTLPKVAAE VAAPLSQAKK ITMVSSGTGD IGAAKLTGEV LSIVNKVPEL VKNITGVDIA RSVHAG
	Isoform Long [UniParc]. Checksum: 31F87AF7C99F1601		430	47,523
	10 20 30 40 50 60 MTWGFVTCGP NEALVVSGCC YMKPLLVPGG RAFVWPVGQQ VQRISLNTMT LQVESPCVYT 70 80 90 100 110 120 SQGVPISVTG IAQVKVQGQN EDMLLTACEQ FLGKSEAEIN HIALVTLEGH QRAIMGSMTV 130 140 150 160 170 180 EEIYKDRKKF SKQVFEVASS DLANMGITVV SYTIKDLRDE EGDSKGYLRS LGMARTAEVK 190 200 210 220 230 240 RDARIGEAEA RAEAHIKEAI AEEQRMAARF LNDTDIAKAQ RDFELKKAAY DVEVQTKKAE 250 260 270 280 290 300 AEMAYELQAA KTKQRIKEEQ MQVKVIERTQ EIAVQEQEIM RRERELEATI RRPAEAEKFR 310 320 330 340 350 360 MEKLAEANKQ RVVMEAEAEA ESIRIRGEAE AFAIAAKAKA EAEQMAMKAE AYREYREAAM 370 380 390 400 410 420 VEMLLDTLPK VAAEVAAPLS QAKKITMVSS GTGDIGAAKL TGEVLSIVNK VPELVKNITG 430 VDIARSVHAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, sequence and developmental expression of invertebrate flotillins from Drosophila melanogaster." Galbiati F., Volonte D., Goltz J.S., Steele Z., Sen J., Jurcsak J., Stein D., Stevens L., Lisanti M.P. Gene 210:229-237(1998) [PubMed: 9573373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF044734 mRNA. Translation: AAC39012.1. AE013599 Genomic DNA. Translation: AAF58120.1. AE013599 Genomic DNA. Translation: AAF58121.1. AY058794 mRNA. Translation: AAL14023.1.
RefSeq	NP_477358.1. NP_725476.1.
UniGene	Dm.2957
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O61491.
Genome annotation databases
Ensembl	CG8200. Drosophila melanogaster. [Contig view]
GeneID	36726.
KEGG	dme:Dmel_CG8200.
Organism-specific databases
FlyBase	FBgn0024754. Flo.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-004785-MON.
Gene expression databases
ArrayExpress	O61491.
GermOnline	CG8200. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001107. Band_7. IPR004851. Flotillin. [Graphical view]
Pfam	PF01145. Band_7. 1 hit. PF03149. Flotillin. 1 hit. [Graphical view]
SMART	SM00244. PHB. 1 hit. [Graphical view]
ProDom	O61491. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

FLOT1_DROME

Accession

Primary (citable) accession number: O61491
Secondary accession number(s): Q9V7D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	August 1, 1998
Last modified:	July 22, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project