Reviewed, UniProtKB/Swiss-Prot O67323 (SYA_AQUAE)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	aq_1293

Organism

Aquifex aeolicus [Complete proteome] [HAMAP]

Taxonomic identifier

63363 [NCBI]

Taxonomic lineage

Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex

Protein attributes

Sequence length	867 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 867

867

Alanyl-tRNA synthetase

Secondary structure

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867

Helix Strand Turn

Details...

Helix

5 – 18

14

Helix

45 – 47

3

Helix

48 – 51

4

Beta strand

60 – 69

10

Turn

78 – 83

6

Beta strand

84 – 86

3

Beta strand

89 – 101

13

Helix

104 – 117

14

Helix

123 – 125

3

Beta strand

126 – 131

6

Helix

135 – 142

8

Turn

143 – 145

3

Helix

149 – 151

3

Beta strand

152 – 155

4

Helix

157 – 160

4

Beta strand

161 – 179

19

Helix

186 – 189

4

Beta strand

190 – 203

14

Beta strand

209 – 222

14

Helix

223 – 230

8

Helix

236 – 238

3

Turn

240 – 242

3

Helix

243 – 253

11

Helix

261 – 282

22

Helix

291 – 309

19

Helix

317 – 328

12

Turn

329 – 331

3

Helix

335 – 373

39

Helix

381 – 389

9

Helix

395 – 403

9

Turn

404 – 406

3

Helix

411 – 425

15

Helix

446 – 449

4

Turn

450 – 452

3

Sequences

	Sequence		Length	Mass (Da)
	O67323-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: E38C6D376643A149	FASTA	867	98,436
	10 20 30 40 50 60 MSLSAHEIRE LFLSFFEKKG HTRVKSAPLV PENDPTLLFV NAGMVPFKNV FLGLEKRPYK 70 80 90 100 110 120 RATSCQKCLR VSGKHNDLEQ VGYTSRHHTF FEMLGNFSFG DYFKKEAIEY AWEFVTEVLK 130 140 150 160 170 180 LPKEKLYVSV YKDDEEAYRI WNEHIGIPSE RIWRLGEEDN FWQMGDVGPC GPSSEIYVDR 190 200 210 220 230 240 GEEYEGDERY LEIWNLVFMQ YNRDENGVLT PLPHPNIDTG MGLERIASVL QGKNSNFEID 250 260 270 280 290 300 IIFPLIQFGE EVSGKKYGEK FETDVALRVI ADHLRAITFA ISDGVIPSNE GRGYVIRRIL 310 320 330 340 350 360 RRAMRFGYKL GIENPFLYKG VDLVVDIMKE PYPELELSRE FVKGIVKGEE KRFIKTLKAG 370 380 390 400 410 420 MEYIQEVIQK ALEEGRKTLS GKEVFTAYDT YGFPVDLIDE IAREKGLGID LEGFQCELEE 430 440 450 460 470 480 QRERARKHFK VEAKKVKPVY SHLKELGKTS AFVGYEHMEW ESQVVGLVKG EGLVSELKEG 490 500 510 520 530 540 EEGEVVLKET PFYPEGGGQI GDAGIIESDK ALFKVEDTQK PTEGIIVHIG KVLKGTLKVG 550 560 570 580 590 600 DTVHARVDKE RRWDIMRNHT ATHLLHAALR NVLGEHVRQA GSLVADKYLR FDFTHFSALT 610 620 630 640 650 660 EEELKRVEEL VNEKIRENLP VNVMEMAYDE ALKTGAIAIF EEKYGERVRV ISCGEFSKEL 670 680 690 700 710 720 CGGTHVSATG DIGYFKIISE SSVGAGVRRI VAQTGRWSVE TAFKEHQTLK KASSALGVGE 730 740 750 760 770 780 EEVIQKIEEL KEEIKDRERE IQRLKQELLK LQIREVVKEE NVGDFTLHYG VFEEVEPEEL 790 800 810 820 830 840 RNLADMLRQR TKKDVVFIAS RKGDKINFVI GVSKEISDKV NAKEVIREVG KVLKGGGGGR 850 860 ADLAQGGGKA PDKFPEAVKL LKEILSG

References

[1]

"The complete genome of the hyperthermophilic bacterium Aquifex aeolicus." Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L., Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R., Feldman R.A., Short J.M., Olsen G.J., Swanson R.V. Nature 392:353-358(1998) [PubMed: 9537320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VF5.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000657 Genomic DNA. Translation: AAC07289.1.

PIR

H70411.

RefSeq

NP_213887.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RIQ	X-ray	2.14	A	1-454	[»]
1YFR	X-ray	2.15	A/B	1-454	[»]
1YFS	X-ray	2.08	A/B	1-454	[»]
1YFT	X-ray	2.23	A	1-454	[»]
1YGB	X-ray	2.48	A	1-454	[»]

ModBase

Search...

Genome annotation databases

GeneID

1192761.

GenomeReviews

Gene locus aq_1293 in contig AE000657_GR.

KEGG

aae:aq_1293.

NMPDR

fig|224324.1.peg.902.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O67323.

Enzyme and pathway databases

BioCyc

AAEO224324:AQ_1293-MON.

Family and domain databases

HAMAP

MF_00036.
[Tree]

InterPro

IPR002318. Ala-tRNA-synth_IIc.
IPR003156. Pesterase_DHHA1.
IPR012947. tRNA_SAD.
[Graphical view]

Pfam

PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]

PRINTS

PR00980. TRNASYNTHALA.

TIGRFAMs

TIGR00344. alaS. 1 hit.

PROSITE

PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

ProDom

O67323.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

ProtoNet

Search...

Entry information

Entry name

SYA_AQUAE

Accession

Primary (citable) accession number: O67323

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	July 22, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)