Reviewed, UniProtKB/Swiss-Prot O68556 (MTB1_BACSU)

Names and origin

Protein names

Recommended name:
    Modification methylase BglI
      Short name=M.BglI
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase BglI
    BglI modification methyltransferase

Gene names

Name:

bglIM

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GCCNNNNNGGC, causes specific methylation on a C on both strands, and protects the DNA from cleavage by the BglI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 348	348	Modification methylase BglI

Sequences

	Sequence		Length	Mass (Da)
	O68556-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7B08004E0DB7E707	FASTA	348	40,242
	10 20 30 40 50 60 MNNHSYLKKN SFHEGDAREL LKCIEEESIA LSVWSPPYHV GKKYEEGQTY EQWSSLLTKV 70 80 90 100 110 120 IALHYPILKP GGFLVINIDD ILAFPDPRMP RFQAVNLKKH RVSVTREDIL NALKLEPELT 130 140 150 160 170 180 KYQLAKKFNC SEQTIERRLK GNNIRGGKYN VQTKVKLAGP VLEKAAEEAG LYLYDRRIWA 190 200 210 220 230 240 KDPAWQNSQW HSNSYKAVSE FEHLYIFWKP GETVIDRNKL SKEEWASWAS RGIWYIPSVR 250 260 270 280 290 300 KNDDHEAKFP LLLPQRLIKL LTQKGDTVLD CFMGSGTTAV AALSESRNFI GIEKEPKYIQ 310 320 330 340 LSNKNVETFY ISRNKEASKI KETNHSVTDE KKKAEQLELL LEENENSL

References

[1]	"Crystal structure of restriction endonuclease BGlI bound to its interrupted DNA recognition sequence." Newman M., Lunnen K.D., Wilson G.G., Greci J., Schildkraut I., Philips S.E.V. EMBO J. 17:5466-5467(1998) [PubMed: 9736624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF050216 Genomic DNA. Translation: AAC63974.1.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3303. M.BglI.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR001091. MeTrfase_CN4. IPR002941. N6/N4_Mtase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE. PR00508. S21N4MTFRASE.
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTB1_BACSU

Accession

Primary (citable) accession number: O68556

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	August 1, 1998
Last modified:	September 2, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)