Reviewed, UniProtKB/Swiss-Prot O70291 (GRK4_MOUSE)

Names and origin

Protein names

Recommended name:
    G protein-coupled receptor kinase 4
    EC=2.7.11.16
Alternative name(s):
    G protein-coupled receptor kinase GRK4

Gene names

Name:	Grk4
Synonyms:	Gprk2l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically phosphorylates the activated forms of G protein-coupled receptors.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 574	574	G protein-coupled receptor kinase 4
Regions
	Domain	51 – 171	121	RGS
	Domain	186 – 448	263	Protein kinase
	Domain	449 – 514	66	AGC-kinase C-terminal
	Nucleotide binding	192 – 200	9	ATP By similarity
	Region	1 – 153	153	N-terminal
Sites
	Active site	311	1	Proton acceptor By similarity
	Binding site	215	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70291-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7E2892EE9C3DEFB6	FASTA	574	66,912
	10 20 30 40 50 60 MELENMVANN LLLKARLGFN KQTGRSKKWR ELLKFPPVSM CTELRWSIEK DFSSLCDKQP 70 80 90 100 110 120 IGRLLFRQFC DTKPDLKRCI EFLDAVAEYE VTIEEEQREF GLAIFSRFFK EKSEVPLPEI 130 140 150 160 170 180 PPDIVKECKW NLKQNSPSQN VFEECAGIVC KYLSETPFEE YQESTYFNRF LQWKWLERRP 190 200 210 220 230 240 VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK 250 260 270 280 290 300 LHSRFVVSLA YTYETKDALC LVLTIMNGGD LKYHIYNLGD PGFEEPRAVF YAAELCCGLE 310 320 330 340 350 360 DLQRKRIVYR DLKPENILLD DHGHIRISDL GLAMEVPEGE MVRGRVGTVG YMAPEIINHE 370 380 390 400 410 420 KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREELERR VKNETEEYSE RFSEDAKSIC 430 440 450 460 470 480 SMLLIKDPSK RLGCQRDGVS AVKQHPIFKD INFSRLEANM LDPPFIPDPQ AIYCRNILDI 490 500 510 520 530 540 GQFSVVKGVN LDTNDEIFYA EFATGSVTIP WQNEMIESGC FKDLNENEDD LSSLEKYKMC 550 560 570 SSILRPKRNF FRRLFRRTGC LNIALSEERE PTEH

References

[1]	"The GRK4 subfamily of G protein-coupled receptor kinases. Alternative splicing, gene organization, and sequence conservation." Premont R.T., Macrae A.D., Aparicio S.A., Kendall H.E., Welch J.E., Lefkowitz R.J. J. Biol. Chem. 274:29381-29389(1999) [PubMed: 10506199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF040745 mRNA. Translation: AAC09266.1.
UniGene	Mm.117076
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
PTM databases
PhosphoSite	O70291.
Genome annotation databases
Ensembl	ENSMUSG00000052783. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:95801. Grk4.
Phylogenomic databases
HOGENOM	O70291.
HOVERGEN	O70291.
Gene expression databases
ArrayExpress	O70291.
CleanEx	MM_GRK4.
GermOnline	ENSMUSG00000052783. Mus musculus.
Family and domain databases
InterPro	IPR000239. GPCR_kinase. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR000342. Regulat_G_prot_signal. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

GRK4_MOUSE

Accession

Primary (citable) accession number: O70291

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	August 1, 1998
Last modified:	July 22, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)