Reviewed, UniProtKB/Swiss-Prot O70370 (CATS_MOUSE)

Names and origin

Protein names

Cathepsin S [Precursor]
Also known as:
     EC 3.4.22.27

Gene names

Name:	Ctss
Synonyms:	Cats

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein existence

Evidence at transcript level.

General annotation (Comments)

Function	Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Catalytic activity	Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.
Subcellular location	Lysosome.
Tissue specificity	Widely expressed with highest expression found in non-skeletal tissues. Relatively high levels found in skeletal tissues.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Glycoprotein Zymogen
Technical term	3D-structure
Gene Ontology (GO)
Biological process	proteolysis Inferred from direct assay. Source: MGI
Cellular component	lysosome Inferred from direct assay. Source: MGI membrane Inferred from direct assay. Source: MGI
Molecular function	cathepsin S activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 17

17

Potential

Propeptide

18 – 122

105

Activation peptide Potential

Chain

123 – 340

218

Cathepsin S

Sites

Active site

147

1

By similarity

Active site

287

1

By similarity

Active site

307

1

By similarity

Amino acid modifications

Glycosylation

120

1

N-linked (GlcNAc...) Potential

Disulfide bond

134 ↔ 233

By similarity

Disulfide bond

144 ↔ 189

By similarity

Disulfide bond

178 ↔ 222

By similarity

Disulfide bond

281 ↔ 329

By similarity

Natural variations

Natural variant

218

1

T → M

Experimental info

Sequence conflict

1 – 29

29

MRAPG…QLQRD → MAVLDAPGVLCGNGATAER Ref.2

Sequence conflict

34

1

Y → H in CAA05360. Ref.2

Sequence conflict

97

1

S → L in CAA05360. Ref.2

Sequence conflict

106

1

S → P in CAA05360. Ref.2

Sequence conflict

146

1

A → S Ref.3

Secondary structure

1

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340

Helix Strand Turn

Details...

Helix

30 – 40

11

Helix

48 – 74

27

Beta strand

78 – 81

4

Beta strand

87 – 89

3

Helix

91 – 97

7

Beta strand

110 – 115

6

Helix

129 – 132

4

Helix

147 – 164

18

Helix

172 – 178

7

Turn

182 – 185

4

Helix

194 – 204

11

Beta strand

207 – 209

3

Turn

210 – 212

3

Helix

226 – 228

3

Beta strand

229 – 231

3

Helix

244 – 253

10

Beta strand

257 – 261

5

Helix

266 – 269

4

Beta strand

271 – 276

6

Beta strand

288 – 298

11

Beta strand

301 – 307

7

Turn

311 – 313

3

Beta strand

319 – 323

5

Beta strand

325 – 328

4

Helix

329 – 331

3

Sequences

	Sequence		Length	Mass (Da)
	O70370-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 068E61126E2E0C0E	FASTA	340	38,438
	10 20 30 40 50 60 MRAPGHAAIR WLFWMPLVCS VAMEQLQRDP TLDYHWDLWK KTHEKEYKDK NEEEVRRLIW 70 80 90 100 110 120 EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEISCRM GALRISRQSP KTVTFRSYSN 130 140 150 160 170 180 RTLPDTVDWR EKGCVTEVKY QGSCGACWAF SAVGALEGQL KLKTGKLISL SAQNLVDCSN 190 200 210 220 230 240 EEKYGNKGCG GGYMTEAFQY IIDNGGIEAD ASYPYKATDE KCHYNSKNRA ATCSRYIQLP 250 260 270 280 290 300 FGDEDALKEA VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK 310 320 330 340 DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI

References

[1]	Doh-ura K. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/Sv and BALB/c. Tissue: Brain.
[2]	Rommerskirch W. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[3]	"Cathepsin expression during skeletal development." Soederstroem M., Salminen H., Glumoff V., Kirschke H., Aro H., Vuorio E. Biochim. Biophys. Acta 1446:35-46(1999) [PubMed: 10395917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 144-306, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Cartilage.
[4]	"Gene expression in scrapie. Cloning of a new scrapie-responsive gene and the identification of increased levels of seven other mRNA transcripts." Dandoy-Dron F., Guillo F., Benboudjema L., Deslys J.-P., Lasmesas C., Dormont D., Tovey M.G., Dron M. J. Biol. Chem. 273:7691-7697(1998) [PubMed: 9516475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 296-340. Strain: C57BL/6. Tissue: Brain.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF051732 , AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA. Translation: AAC05781.1.
AF038546 mRNA. Translation: AAB94925.1.
AJ002386 mRNA. Translation: CAA05360.1.
Y18466 mRNA. Translation: CAA77184.1.
AJ223208 mRNA. Translation: CAA11182.1.

UniGene

Mm.3619

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M0H	model	-	A	1-340	[»]

SMR

O70370. Positions 26-340.

ModBase

Search...

Protein family/group databases

MEROPS

C01.034.

Genome annotation databases

Ensembl

ENSMUSG00000038642. Mus musculus. [Contig view]

Organism-specific databases

MGI

MGI:107341. Ctss.

Phylogenomic databases

HOVERGEN

O70370.

Gene expression databases

ArrayExpress

O70370.

CleanEx

MM_CTSS.

GermOnline

ENSMUSG00000038642. Mus musculus.

Family and domain databases

InterPro

IPR000169. Pept_cys_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]

PANTHER

PTHR12411. Peptidase_C1A. 1 hit.

Pfam

PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

ProDom

PD000158. Peptidase_C1. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

CATS_MOUSE

Accession

Primary (citable) accession number: O70370
Secondary accession number(s): O54973

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	August 1, 1998
Last modified:	June 10, 2008
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)