Reviewed, UniProtKB/Swiss-Prot O75688 (PPM1B_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 1B
    EC=3.1.3.16
Alternative name(s):
    Protein phosphatase 2C isoform beta
      Short name=PP2C-beta

Gene names

Name:	PPM1B
Synonyms:	PP2CB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro.
Catalytic activity	A phosphoprotein + H(2)O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Tissue specificity	Highly expressed in heart and skeletal muscle.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	protein serine/threonine phosphatase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine phosphatase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ANXA1	P04083	1	EBI-1047039,EBI-354007
ANXA2	P07355	1	EBI-1047039,EBI-352622
ARRB1	P49407	1	EBI-1047039,EBI-743313
ARRB2	P32121	1	EBI-1047039,EBI-714559
GSN	P06396	1	EBI-1047039,EBI-351506
KRT18	P05783	1	EBI-1047039,EBI-297888
PABPC4	Q13310	1	EBI-1047039,EBI-372844
RASGRP1	O95267	1	EBI-1047039,EBI-1054956
S100A8	P05109	1	EBI-1047039,EBI-355281
VCP	Q5VYM0	1	EBI-1047039,EBI-1046421
VIL1	P09327	1	EBI-1047039,EBI-1047253

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Notes: Additional isoforms seem to exist.

Isoform Beta-1 (identifier: O75688-1)

Also known as: Beta-X; PPM1B2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta-2 (identifier: O75688-2)

Also known as: PPM1B1;

The sequence of this isoform differs from the canonical sequence as follows:
     379-387: ASDEAEESG → GAGDLEDPW
     388-479: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 479

479

Protein phosphatase 1B

PRO_0000057746

Sites

Metal binding

60

1

Manganese 1 By similarity

Metal binding

60

1

Manganese 2 By similarity

Metal binding

61

1

Manganese 1; via carbonyl oxygen By similarity

Metal binding

243

1

Manganese 2 By similarity

Metal binding

286

1

Manganese 2 By similarity

Natural variations

Alternative sequence

379 – 387

9

ASDEAEESG → GAGDLEDPW in isoform Beta-2.

VSP_005087

Alternative sequence

388 – 479

92

Missing in isoform Beta-2.

VSP_005088

Secondary structure

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479

Helix Strand Turn

Details...

Beta strand

9 – 19

11

Beta strand

22 – 31

10

Beta strand

33 – 35

3

Beta strand

38 – 46

9

Turn

47 – 49

3

Beta strand

50 – 63

14

Helix

66 – 80

15

Turn

83 – 85

3

Helix

99 – 118

20

Turn

121 – 123

3

Beta strand

134 – 139

6

Beta strand

141 – 151

11

Beta strand

153 – 158

6

Beta strand

161 – 165

5

Helix

174 – 182

9

Turn

193 – 195

3

Helix

205 – 207

3

Helix

215 – 217

3

Beta strand

218 – 221

4

Beta strand

225 – 230

6

Beta strand

235 – 241

7

Helix

243 – 246

4

Helix

251 – 262

12

Helix

268 – 281

14

Beta strand

288 – 294

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform Beta-1 (Beta-X) (PPM1B2) [UniParc]. Last modified November 1, 1998. Version 1. Checksum: A3A5797AD263DFBD	FASTA	479	52,643
	10 20 30 40 50 60 MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL EDWSFFAVYD 70 80 90 100 110 120 GHAGSRVANY CSTHLLEHIT TNEDFRAAGK SGSALELSVE NVKNGIRTGF LKIDEYMRNF 130 140 150 160 170 180 SDLRNGMDRS GSTAVGVMIS PKHIYFINCG DSRAVLYRNG QVCFSTQDHK PCNPREKERI 190 200 210 220 230 240 QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFIIL 250 260 270 280 290 300 ACDGIWDVMS NEELCEYVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFSNAPK 310 320 330 340 350 360 VSDEAVKKDS ELDKHLESRV EEIMEKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 370 380 390 400 410 420 NVIEAVYSRL NPHRESDGAS DEAEESGSQG KLVEALRQMR INHRGNYRQL LEEMLTSYRL 430 440 450 460 470 AKVEGEESPA EPAATATSSN SDAGNPVTMQ ESHTESESGL AELDSSNEDA GTKMSGEKI
	Isoform Beta-2 (PPM1B1). Checksum: 56A644A4E5E2B12B		387	42,771
	10 20 30 40 50 60 MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL EDWSFFAVYD 70 80 90 100 110 120 GHAGSRVANY CSTHLLEHIT TNEDFRAAGK SGSALELSVE NVKNGIRTGF LKIDEYMRNF 130 140 150 160 170 180 SDLRNGMDRS GSTAVGVMIS PKHIYFINCG DSRAVLYRNG QVCFSTQDHK PCNPREKERI 190 200 210 220 230 240 QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFIIL 250 260 270 280 290 300 ACDGIWDVMS NEELCEYVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFSNAPK 310 320 330 340 350 360 VSDEAVKKDS ELDKHLESRV EEIMEKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 370 380 NVIEAVYSRL NPHRESDGGA GDLEDPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning expression and tissue distribution of human PP2Cbeta." Marely A.E., Kline A., Crabtree G., Sullivan J.E., Beri R.K. FEBS Lett. 431:121-124(1998) [PubMed: 9684878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-1). Tissue: Liver.
[2]	"Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2Calpha and beta2 isoforms." Cheng A., Kaldis P., Solomon M.J. J. Biol. Chem. 275:34744-34749(2000) [PubMed: 10934208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-2).
[3]	"Protein phosphatase 1B. Cloning and characterization of two major transcripts generated by alternative use of 3' exons." Seroussi E., Shani N., Hayut A., Faier S., Ben-Meir D., Divinski I., Smorodinsky N.I., Lavi S. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BETA-1 AND BETA-2).
[4]	"A novel gene expressed in human adrenal gland." Peng Y., Gu Y., Li Y., Fu S., Gu J., Zhang L., Jiang C., Yu Y., Han Z., Wang Y., Chen Z., Fu G. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-2). Tissue: Adrenal gland.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA-1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ005801 mRNA. Translation: CAA06704.1.
AF294792 mRNA. Translation: AAG02232.1.
AJ271832 mRNA. Translation: CAC27992.1.
AJ271835 mRNA. Translation: CAC27993.1.
AF136972 mRNA. Translation: AAG49433.1.
BC064381 mRNA. Translation: AAH64381.1.

RefSeq

NP_002697.1.

UniGene

Hs.416769

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P8E	X-ray	1.82	A/B	2-297	[»]

SMR

O75688. Positions 2-373.

ModBase

Search...

Protein-protein interaction databases

IntAct

O75688. 15 interactions.

PTM databases

PhosphoSite

O75688.

Proteomic databases

PRIDE

O75688.

Genome annotation databases

Ensembl

ENSG00000138032. Homo sapiens. [Contig view]

GeneID

5495.

Organism-specific databases

GeneCards

GC02P044307.

H-InvDB

HIX0002014.

HGNC

HGNC:9276. PPM1B.

HPA

HPA016745.

MIM

603770. gene.

PharmGKB

PA33604.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O75688.

HOVERGEN

O75688.

Gene expression databases

ArrayExpress

O75688.

CleanEx

HS_PPM1B.

GermOnline

ENSG00000138032. Homo sapiens.

Family and domain databases

InterPro

IPR015655. PP2C.
IPR001932. PP2C-related.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR014045. PP2C_N.
[Graphical view]

Gene3D

G3DSA:3.60.40.10. PP2C-related. 1 hit.
G3DSA:1.10.10.430. PP2C_C. 1 hit.

PANTHER

PTHR13832. PP2C. 1 hit.

Pfam

PF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]

SMART

SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]

PROSITE

PS01032. PP2C. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

O75688.

NextBio

21250.

SOURCE

Search...

Entry information

Entry name

PPM1B_HUMAN

Accession

Primary (citable) accession number: O75688
Secondary accession number(s): Q9HAY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)