Reviewed, UniProtKB/Swiss-Prot O75762 (TRPA1_HUMAN)

Names and origin

Protein names

Recommended name:
    Transient receptor potential cation channel subfamily A member 1
Alternative name(s):
    Ankyrin-like with transmembrane domains protein 1
    Transformation-sensitive protein p120

Gene names

Name:	TRPA1
Synonyms:	ANKTM1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1119 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor-activated non-selective cation channel involved in detection of sensations such as cold, pain or sounds perception. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Also involved in pain sensation triggered by capsaicin, the pungent ingredient in chilli peppers. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system By similarity.
Subcellular location	Membrane; Multi-pass membrane proteinProbable.
Tissue specificity	Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells. Ref.1
Miscellaneous	Inhibited by ruthenium red, a potent blocker of TRPV channels By similarity.
Sequence similarities	Belongs to the transient receptor family. Contains 15 ANK repeats.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	ANK repeat Repeat Transmembrane
Molecular function	Ionic channel
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	ion transport Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1119	1119	Transient receptor potential cation channel subfamily A member 1		PRO_0000215369
Regions
	Topological domain	1 – 719	719	Cytoplasmic Potential
	Transmembrane	720 – 740	21	1 Potential
	Topological domain	741 – 772	32	Extracellular Potential
	Transmembrane	773 – 793	21	2 Potential
	Topological domain	794 – 805	12	Cytoplasmic Potential
	Transmembrane	806 – 826	21	3 Potential
	Topological domain	827 – 829	3	Extracellular Potential
	Transmembrane	830 – 850	21	4 Potential
	Topological domain	851 – 873	23	Cytoplasmic Potential
	Transmembrane	874 – 894	21	5 Potential
	Topological domain	895 – 940	46	Extracellular Potential
	Transmembrane	941 – 961	21	6 Potential
	Topological domain	962 – 1119	158	Cytoplasmic Potential
	Repeat	62 – 92	31	ANK 1
	Repeat	97 – 126	30	ANK 2
	Repeat	130 – 160	31	ANK 3
	Repeat	164 – 193	30	ANK 4
	Repeat	197 – 226	30	ANK 5
	Repeat	238 – 267	30	ANK 6
	Repeat	271 – 301	31	ANK 7
	Repeat	308 – 337	30	ANK 8
	Repeat	341 – 370	30	ANK 9
	Repeat	412 – 441	30	ANK 10
	Repeat	445 – 474	30	ANK 11
	Repeat	481 – 510	30	ANK 12
	Repeat	513 – 542	30	ANK 13
	Repeat	547 – 576	30	ANK 14
	Repeat	579 – 609	31	ANK 15
Amino acid modifications
	Glycosylation	747	1	N-linked (GlcNAc...) Potential
	Glycosylation	753	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	3	1	R → C: dbSNP rs13268757. Ref.1		VAR_020660
	Natural variant	58	1	R → T: dbSNP rs16937976. Ref.1		VAR_047471
	Natural variant	179	1	E → K: dbSNP rs920829.		VAR_020661
	Natural variant	186	1	N → K: dbSNP rs7819749. Ref.2		VAR_020662
	Natural variant	1018	1	H → R: dbSNP rs959976.		VAR_020663

Sequences

	Sequence		Length	Mass (Da)
	O75762-1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: FBFE5C6C105B0B3C	FASTA	1,119	127,487
	10 20 30 40 50 60 MKRSLRKMWR PGEKKEPQGV VYEDVPDDTE DFKESLKVVF EGSAYGLQNF NKQKKLKRCD 70 80 90 100 110 120 DMDTFFLHYA AAEGQIELME KITRDSSLEV LHEMDDYGNT PLHCAVEKNQ IESVKFLLSR 130 140 150 160 170 180 GANPNLRNFN MMAPLHIAVQ GMNNEVMKVL LEHRTIDVNL EGENGNTAVI IACTTNNSEA 190 200 210 220 230 240 LQILLNKGAK PCKSNKWGCF PIHQAAFSGS KECMEIILRF GEEHGYSRQL HINFMNNGKA 250 260 270 280 290 300 TPLHLAVQNG DLEMIKMCLD NGAQIDPVEK GRCTAIHFAA TQGATEIVKL MISSYSGSVD 310 320 330 340 350 360 IVNTTDGCHE TMLHRASLFD HHELADYLIS VGADINKIDS EGRSPLILAT ASASWNIVNL 370 380 390 400 410 420 LLSKGAQVDI KDNFGRNFLH LTVQQPYGLK NLRPEFMQMQ QIKELVMDED NDGCTPLHYA 430 440 450 460 470 480 CRQGGPGSVN NLLGFNVSIH SKSKDKKSPL HFAASYGRIN TCQRLLQDIS DTRLLNEGDL 490 500 510 520 530 540 HGMTPLHLAA KNGHDKVVQL LLKKGALFLS DHNGWTALHH ASMGGYTQTM KVILDTNLKC 550 560 570 580 590 600 TDRLDEDGNT ALHFAAREGH AKAVALLLSH NADIVLNKQQ ASFLHLALHN KRKEVVLTII 610 620 630 640 650 660 RSKRWDECLK IFSHNSPGNK CPITEMIEYL PECMKVLLDF CMLHSTEDKS CRDYYIEYNF 670 680 690 700 710 720 KYLQCPLEFT KKTPTQDVIY EPLTALNAMV QNNRIELLNH PVCKEYLLMK WLAYGFRAHM 730 740 750 760 770 780 MNLGSYCLGL IPMTILVVNI KPGMAFNSTG IINETSDHSE ILDTTNSYLI KTCMILVFLS 790 800 810 820 830 840 SIFGYCKEAG QIFQQKRNYF MDISNVLEWI IYTTGIIFVL PLFVEIPAHL QWQCGAIAVY 850 860 870 880 890 900 FYWMNFLLYL QRFENCGIFI VMLEVILKTL LRSTVVFIFL LLAFGLSFYI LLNLQDPFSS 910 920 930 940 950 960 PLLSIIQTFS MMLGDINYRE SFLEPYLRNE LAHPVLSFAQ LVSFTIFVPI VLMNLLIGLA 970 980 990 1000 1010 1020 VGDIAEVQKH ASLKRIAMQV ELHTSLEKKL PLWFLRKVDQ KSTIVYPNKP RSGGMLFHIF 1030 1040 1050 1060 1070 1080 CFLFCTGEIR QEIPNADKSL EMEILKQKYR LKDLTFLLEK QHELIKLIIQ KMEIISETED 1090 1100 1110 DDSHCSFQDR FKKEQMEQRN SRWNTVLRAV KAKTHHLEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An ankyrin-like protein with transmembrane domains is specifically lost after oncogenic transformation of human fibroblasts." Jaquemar D., Schenker T., Trueb B. J. Biol. Chem. 274:7325-7333(1999) [PubMed: 10066796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANTS CYS-3 AND THR-58. Tissue: Lung.
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LYS-186.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y10601 mRNA. Translation: CAA71610.1. AC022867 Genomic DNA. No translation available.
UniGene	Hs.667156
3D structure databases
HSSP	HSSP built from PDB template 1UOH based on UniProtKB O75832.
ModBase	Search...
PTM databases
PhosphoSite	O75762.
Genome annotation databases
Ensembl	ENSG00000104321. Homo sapiens. [Contig view]
Organism-specific databases
GeneCards	GC08M073096.
H-InvDB	HIX0034373.
HGNC	HGNC:497. TRPA1.
MIM	604775. gene.
PharmGKB	PA24808.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75762.
HOVERGEN	O75762.
Gene expression databases
ArrayExpress	O75762.
CleanEx	HS_TRPA1.
GermOnline	ENSG00000104321. Homo sapiens.
Family and domain databases
InterPro	IPR002110. ANK. IPR005821. Ion_trans. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 2 hits.
Pfam	PF00023. Ank. 13 hits. PF00520. Ion_trans. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 14 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 9 hits. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00825. Menthol.
LinkHub	O75762.
SOURCE	Search...

Entry information

Entry name

TRPA1_HUMAN

Accession

Primary (citable) accession number: O75762
Secondary accession number(s): A6NIN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	November 25, 2008
Last modified:	December 16, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)