Reviewed, UniProtKB/Swiss-Prot O75832 (PSD10_HUMAN)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 10
Alternative name(s):
    26S proteasome regulatory subunit p28
    Gankyrin

Gene names

Name:

PSMD10

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Subunit structure	Component of the PA700 complex.
Sequence similarities	Contains 7 ANK repeats.

Ontologies

Keywords
Cellular component	Proteasome
Domain	ANK repeat Repeat
PTM	Acetylation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytoskeleton Inferred from direct assay. Source: HPA cytosol Inferred from electronic annotation. Source: UniProtKB-KW proteasome regulatory particle Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DOK2	O60496	1	EBI-752185,EBI-1046024
ELOVL1	Q9BW60	1	EBI-752185,EBI-1050331
HIST1H2BC	P62807	1	EBI-752185,EBI-354552
MAGEA4	P43358	4	EBI-752185,EBI-743122
PAAF1	Q9BRP4	1	EBI-752185,EBI-1056358
PSMC1	P62191	1	EBI-752185,EBI-357598
PSMC2	P35998	1	EBI-752185,EBI-359710
PSMC3	P17980	1	EBI-752185,EBI-359720
PSMC4	P43686	2	EBI-752185,EBI-743997
PSMC5	P62195	1	EBI-752185,EBI-357745
PSMC6	P62333	1	EBI-752185,EBI-357669
PSMD1	Q99460	1	EBI-752185,EBI-357874
PSMD11	O00231	1	EBI-752185,EBI-357816
PSMD12	O00232	1	EBI-752185,EBI-359733
PSMD2	Q13200	1	EBI-752185,EBI-357648
PSMD3	O43242	1	EBI-752185,EBI-357622
PSMD4	P55036	1	EBI-752185,EBI-359318
PSMD6	Q15008	1	EBI-752185,EBI-359701
PSMD7	P51665	1	EBI-752185,EBI-357659
RB1	P06400	1	EBI-752185,EBI-491274

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 226

226

26S proteasome non-ATPase regulatory subunit 10

PRO_0000067045

Regions

Repeat

3 – 36

34

ANK 1

Repeat

37 – 69

33

ANK 2

Repeat

70 – 102

33

ANK 3

Repeat

103 – 135

33

ANK 4

Repeat

136 – 168

33

ANK 5

Repeat

169 – 201

33

ANK 6

Repeat

202 – 226

25

ANK 7

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.5

Secondary structure

1

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226

Helix Strand Turn

Details...

Beta strand

6 – 8

3

Helix

9 – 15

7

Helix

19 – 28

10

Helix

30 – 34

5

Helix

43 – 50

8

Helix

53 – 62

10

Helix

76 – 83

8

Helix

86 – 94

9

Helix

109 – 115

7

Helix

119 – 127

9

Helix

142 – 148

7

Helix

152 – 160

9

Helix

175 – 181

7

Helix

185 – 193

9

Helix

208 – 211

4

Helix

216 – 224

9

Sequences

	Sequence		Length	Mass (Da)
	O75832-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 57158E33146EC7C8	FASTA	226	24,428
	10 20 30 40 50 60 MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL 70 80 90 100 110 120 QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE 130 140 150 160 170 180 IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC 190 200 210 220 DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome." Hori T., Kato S., Saeki M., DeMartino G.N., Slaughter C.A., Takeuchi J., Toh-e A., Tanaka K. Gene 216:113-122(1998) [PubMed: 9714768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Enhanced expression of a novel tumour marker in the human hepatomas." Higashitsuji H., Fujita J. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Placenta.
[3]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY.
[6]	"Solution structure of the human oncogenic protein gankyrin containing seven ankyrin repeats and analysis of its structure--function relationship." Yuan C., Li J., Mahajan A., Poi M.J., Byeon I.-J., Tsai M.-D. Biochemistry 43:12152-12161(2004) [PubMed: 15379554] [Abstract] Cited for: STRUCTURE BY NMR, DOMAINS ANKYRIN REPEATS.
[7]	"The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53." Krzywda S., Brzozowski A.M., Higashitsuji H., Fujita J., Welchman R., Dawson S., Mayer R.J., Wilkinson A.J. J. Biol. Chem. 279:1541-1545(2004) [PubMed: 14573599] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), ANKYRIN REPEATS.
[8]	"X-ray structure of human gankyrin, the product of a gene linked to hepatocellular carcinoma." Manjasetty B.A., Quedenau C., Sievert V., Bussow K., Niesen F., Delbruck H., Heinemann U. Proteins 55:214-217(2004) [PubMed: 14997555] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 2-226.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB009619 mRNA. Translation: BAA33215.1.
D83197 mRNA. Translation: BAA34594.1.
AL031177 Genomic DNA. Translation: CAA20117.1.
BC011960 mRNA. Translation: AAH11960.1.

RefSeq

NP_002805.1.

UniGene

Hs.522752

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QYM	X-ray	2.80	A	2-226	[»]
1TR4	NMR	-	A	1-226	[»]
1UOH	X-ray	2.00	A	1-226	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O75832. 21 interactions.

PTM databases

PhosphoSite

O75832.

2-D gel databases

OGP

O75832.

Proteomic databases

PRIDE

O75832.

Genome annotation databases

Ensembl

ENSG00000101843. Homo sapiens. [Contig view]

GeneID

5716.

KEGG

hsa:5716.

NMPDR

fig|9606.3.peg.33217.

Organism-specific databases

GeneCards

GC0XM107133.

H-InvDB

HIX0016981.

HGNC

HGNC:9555. PSMD10.

HPA

CAB010434.
HPA002920.

MIM

603480. gene.

PharmGKB

PA33900.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O75832.

HOVERGEN

O75832.

Enzyme and pathway databases

Reactome

REACT_11045. Signaling by Wnt.
REACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_383. DNA Replication.
REACT_6185. HIV Infection.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.

Gene expression databases

ArrayExpress

O75832.

CleanEx

HS_PSMD10.

GermOnline

ENSG00000101843. Homo sapiens.

Family and domain databases

InterPro

IPR002110. ANK.
[Graphical view]

Gene3D

G3DSA:1.25.40.20. ANK. 1 hit.

Pfam

PF00023. Ank. 5 hits.
[Graphical view]

PRINTS

PR01415. ANKYRIN.

SMART

SM00248. ANK. 5 hits.
[Graphical view]

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

O75832.

NextBio

22206.

SOURCE

Search...

Entry information

Entry name

PSD10_HUMAN

Accession

Primary (citable) accession number: O75832

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)