Reviewed, UniProtKB/Swiss-Prot O75843 (AP1G2_HUMAN)

Names and origin

Protein names

Recommended name:
    AP-1 complex subunit gamma-like 2
Alternative name(s):
    Gamma2-adaptin
      Short name=G2ad

Gene names

Name:

AP1G2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-asssisted maturation of hepatitis B virus (HBV). Ref.1 Ref.4 Ref.5
Subunit structure	Probably interacts with AP1S1/Sigma1A-adaptin AP1S2/Sigma1B-adaptin. Probably does not interact with APB1. Interacts with HBV major surface antigen L. Interacts with HBV core protein C in a ubiquitin-dependent manner. Binds ubiquitin. Ref.1 Ref.4 Ref.3
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note= Mainly localized to perinuclear vesicular structures. Colocalizes with HBV major surface antigen L and HBV core protein C in CD63-containing compartments. Colocalizes with HBV major surface antigen L to cis-Golgi-like structures. Ref.1 Ref.4 Ref.3 Ref.2
Tissue specificity	Expressed in all but one (skeletal muscle) tissues examined.
Sequence similarities	Belongs to the adaptor complexes large subunit family. Contains 1 GAE domain.
Caution	Does not appear to be a subunit of the clathrin-associated adaptor protein complex 1 (AP-1).
Sequence caution	The sequence AAC67390.1 differs from that shown. Reason: Frameshift at position 713.

Ontologies

Keywords
Biological process	Host-virus interaction Protein transport Transport
Cellular component	Cytoplasmic vesicle Endosome Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Technical term	3D-structure
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	AP-1 adaptor complex Ref.1 Traceable author statement. Source: UniProtKB endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: HPA
Molecular function	protein binding Inferred from sequence or structural similarity. Source: UniProtKB protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RABGEF1	O18973	1	EBI-373637,EBI-447376	From a different organism.
XRN1	Q8IZH2	1	EBI-373637,EBI-372406

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 785

785

AP-1 complex subunit gamma-like 2

PRO_0000193760

Regions

Domain

665 – 780

116

GAE

Region

369 – 379

11

Essential for ubiquitin-binding

Natural variations

Natural variant

377

1

S → F: dbSNP rs12897422.

VAR_024363

Experimental info

Mutagenesis

369

1

L → G: Greatly diminishes interaction with ubiquitin; when associated with G-372 Ref.4

Mutagenesis

372

1

A → G: Greatly diminishes interaction with ubiquitin; when associated with G-369 Ref.4

Mutagenesis

372

1

A → G: Greatly diminishes interaction with ubiquitin; when associated with G-376

Mutagenesis

376

1

S → G: Greatly diminishes interaction with ubiquitin; when associated with G-372 Ref.4

Sequence conflict

204 – 206

3

ERS → GRN in AAC67390. Ref.2

Sequence conflict

399

1

A → C in AAC67390. Ref.2

Sequence conflict

402

1

A → D in AAC67390. Ref.2

Sequence conflict

416

1

K → T in AAC67390. Ref.2

Sequence conflict

429

1

T → S in AAC67390. Ref.2

Sequence conflict

439 – 441

3

VAN → AGHT in AAC67390. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

785

Helix Strand Turn

Details...

Beta strand

670 – 674

5

Beta strand

676 – 685

10

Beta strand

693 – 702

10

Beta strand

704 – 706

3

Beta strand

708 – 716

9

Beta strand

731 – 733

3

Turn

735 – 737

3

Beta strand

745 – 748

4

Beta strand

757 – 764

8

Beta strand

766 – 776

11

Turn

782 – 784

3

Sequences

	Sequence		Length	Mass (Da)
	O75843-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C189192C9811111C	FASTA	785	87,117
	10 20 30 40 50 60 MVVPSLKLQD LIEEIRGAKT QAQEREVIQK ECAHIRASFR DGDPVHRHRQ LAKLLYVHML 70 80 90 100 110 120 GYPAHFGQME CLKLIASSRF TDKRVGYLGA MLLLDERHDA HLLITNSIKN DLSQGIQPVQ 130 140 150 160 170 180 GLALCTLSTM GSAEMCRDLA PEVEKLLLQP SPYVRKKAIL TAVHMIRKVP ELSSVFLPPC 190 200 210 220 230 240 AQLLHERHHG ILLGTITLIT ELCERSPAAL RHFRKVVPQL VHILRTLVTM GYSTEHSISG 250 260 270 280 290 300 VSDPFLQVQI LRLLRILGRN HEESSETMND LLAQVATNTD TSRNAGNAVL FETVLTIMDI 310 320 330 340 350 360 RSAAGLRVLA VNILGRFLLN SDRNIRYVAL TSLLRLVQSD HSAVQRHRPT VVECLRETDA 370 380 390 400 410 420 SLSRRALELS LALVNSSNVR AMMQELQAFL ESCPPDLRAD CASGILLAAE RFAPTKRWHI 430 440 450 460 470 480 DTILHVLTTA GTHVRDDAVA NLTQLIGGAQ ELHAYSVRRL YNALAEDISQ QPLVQVAAWC 490 500 510 520 530 540 IGEYGDLLLA GNCEEIEPLQ VDEEEVLALL EKVLQSHMSL PATRGYALTA LMKLSTRLCG 550 560 570 580 590 600 DNNRIRQVVS IYGSCLDVEL QQRAVEYDTL FRKYDHMRAA ILEKMPLVER DGPQADEEAK 610 620 630 640 650 660 ESKEAAQLSE AAPVPTEPQA SQLLDLLDLL DGASGDVQHP PHLDPSPGGA LVHLLDLPCV 670 680 690 700 710 720 PPPPAPIPDL KVFEREGVQL NLSFIRPPEN PALLLITITA TNFSEGDVTH FICQAAVPKS 730 740 750 760 770 780 LQLQLQAPSG NTVPARGGLP ITQLFRILNP NKAPLRLKLR LTYDHFHQSV QEIFEVNNLP VESWQ

References

[1]	"Identification and characterization of novel clathrin adaptor-related proteins." Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K. J. Biol. Chem. 273:24693-24700(1998) [PubMed: 9733768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, NEGATIVE INTERACTION WITH APB1, INTERACTION WITH AP1S1 AND AP1S2. Tissue: Liver.
[2]	"Cloning, expression, and localization of a novel gamma-adaptin-like molecule." Lewin D.A., Sheff D., Ooi C.E., Whitney J.A., Yamamoto E., Chicione L.M., Webster P., Bonifacino J.S., Mellman I. FEBS Lett. 435:263-268(1998) [PubMed: 9762922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[3]	"Hepatitis B virus large envelope protein interacts with gamma2-adaptin, a clathrin adaptor-related protein." Hartmann-Stuehler C., Prange R. J. Virol. 75:5343-5351(2001) [PubMed: 11333915] [Abstract] Cited for: INTERACTION WITH HBV MAJOR SURFACE ANTIGEN L, SUBCELLULAR LOCATION.
[4]	"Gamma-adaptin, a novel ubiquitin-interacting adaptor, and Nedd4 ubiquitin ligase control hepatitis B virus maturation." Rost M., Mann S., Lambert C., Doring T., Thome N., Prange R. J. Biol. Chem. 281:29297-29308(2006) [PubMed: 16867982] [Abstract] Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION, SUBCELLULAR LOCATION, INTERACTION WITH HBV CORE PROTEIN AND UBIQUITIN, MUTAGENESIS OF LEU-369; ALA-372 AND SER-376.
[5]	"Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin." Lambert C., Doering T., Prange R. J. Virol. 81:9050-9060(2007) [PubMed: 17553870] [Abstract] Cited for: FUNCTION IN HEPATITIS VIRUS B MATURATION.
[6]	"Solution structure of the alpha adaptin C2 domain from human adapter-related protein complex 1 gamma 2 subunit." RIKEN structural genomics initiative (RSGI) Submitted (JUL-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 662-785.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB015318 mRNA. Translation: BAA33390.1.
AF068706 mRNA. Translation: AAC67390.1. Frameshift.

RefSeq

NP_003908.1.

UniGene

Hs.343244

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E9G	NMR	-	A	662-785	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O75843. 5 interactions.

PTM databases

PhosphoSite

O75843.

Proteomic databases

PeptideAtlas

O75843.

PRIDE

O75843.

Genome annotation databases

Ensembl

ENSG00000213983. Homo sapiens. [Contig view]

GeneID

8906.

KEGG

hsa:8906.

Organism-specific databases

GeneCards

GC14M023098.

H-InvDB

HIX0011545.

HGNC

HGNC:556. AP1G2.

HPA

HPA004106.

MIM

603534. gene.

PharmGKB

PA24846.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O75843.

HOVERGEN

O75843.

Gene expression databases

CleanEx

HS_AP1G2.

GermOnline

ENSG00000092051. Homo sapiens.

Family and domain databases

InterPro

IPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
[Graphical view]

Gene3D

G3DSA:1.25.10.10. ARM-like. 1 hit.
G3DSA:2.60.40.1230. Clathrin_g-adaptin_app. 1 hit.

Pfam

PF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]

PIRSF

PIRSF037094. AP1_complex_gamma. 1 hit.

ProDom

PD021457. Gamma_adaptin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]

PROSITE

PS50180. GAE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

33461.

SOURCE

Search...

Entry information

Entry name

AP1G2_HUMAN

Accession

Primary (citable) accession number: O75843
Secondary accession number(s): O75504

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)