Reviewed, UniProtKB/Swiss-Prot O75915 (PRAF3_HUMAN)

Names and origin

Protein names

Recommended name:
    PRA1 family protein 3
Alternative name(s):
    ADP-ribosylation factor-like protein 6-interacting protein 5
      Short name=ARL-6-interacting protein 5
      Short name=Aip-5
    Glutamate transporter EAAC1-interacting protein
    GTRAP3-18
    Prenylated Rab acceptor protein 2
    Protein JWa
    Dermal papilla-derived protein 11
    JM5
    Putative MAPK-activating protein PM27
    Cytoskeleton-related vitamin A-responsive protein

Gene names

Name:	ARL6IP5
Synonyms:	DERP11, JWA, PRA2, PRAF3
ORF Names:	HSPC127

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic By similarity.
Subunit structure	Forms multimers. Binds to prenylated RAB1A and RAB3A, to the neuronal glutamate transporter SLC1A1/EAAC1, and to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily. Does not bind to VAMP2 By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane proteinBy similarity. CytoplasmBy similarity. Note= Also exists as a soluble form in the cytoplasm. Associated with microtubules By similarity.
Induction	By methyl-beta-cyclodextrin By similarity. Up-regulated upon induced differentiation and in heat stress.
Sequence similarities	Belongs to the PRA1 family.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Membrane
Domain	Transmembrane
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	L-glutamate transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 188	188	PRA1 family protein 3
Regions
	Topological domain	1 – 35	35	Cytoplasmic By similarity
	Transmembrane	36 – 56	21	Potential
	Transmembrane	57 – 77	21	Potential
	Topological domain	78 – 93	16	Cytoplasmic By similarity
	Transmembrane	94 – 114	21	Potential
	Transmembrane	115 – 135	21	Potential
	Topological domain	136 – 188	53	Cytoplasmic By similarity
	Region	136 – 188	53	Targeting to endoplasmic reticulum membrane By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine
Experimental info
	Sequence conflict	28	1	D → G in BAD97286. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	O75915-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 3AA708C6D0901B44	FASTA	188	21,615
	10 20 30 40 50 60 MDVNIAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV AAMMISIVGF 70 80 90 100 110 120 LSPFNMILGG IVVVLVFTGF VWAAHNKDVL RRMKKRYPTT FVMVVMLASY FLISMFGGVM 130 140 150 160 170 180 VFVFGITFPL LLMFIHASLR LRNLKNKLEN KMEGIGLKRT PMGIVLDALE QQEEGINRLT DYISKVKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala." Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M. NeuroReport 13:2079-2084(2002) [PubMed: 12438930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"JM4 is a four-transmembrane protein binding to the CCR5 receptor." Schweneker M., Bachmann A.S., Moelling K. FEBS Lett. 579:1751-1758(2005) [PubMed: 15757671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Neuroblastoma.
[3]	"Molecular cloning of a dermal papilla derived gene." Ikeda A., Yoshimoto M. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hair follicle dermal papilla.
[4]	"A novel differentially displayed vitamin A responsive gene that is cytoskeleton related." Zhou J.W., Di Y.P., Wu R. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cloning of a novel human cDNA homologous to human mRNA for JM4 protein." Zhang X.N., Yu L., Jin L., Hu P.R., Chen S.Y., Zhao S.Y. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"A novel gene expressed in the human adrenal gland." Jiang C., Huang C., Wu T., Peng Y., Gu Y., Zhang L., Zhang C., Li Y., Han Z., Wang Y., Chen Z., Fu G. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[7]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[8]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed: 12761501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung fibroblast.
[9]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow and Brain.
[11]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-9, ACETYLATION AT MET-1. Tissue: Platelet.
[12]	Bienvenut W.V., Claeys D. Submitted (NOV-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-27; 152-158 AND 160-185, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Platelet.
[13]	"Effect of differentiation inducer and heat stress on the expression of JWA protein and Hsp70 of K562 cells." Mao W.G., Li A.P., Ye J., Huang S., Li A.Q., Zhou J.W. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 21:253-256(2003) [PubMed: 14761432] [Abstract] Cited for: INDUCTION.
[14]	"Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)." Fo C.S., Coleman C.S., Wallick C.J., Vine A.L., Bachmann A.S. Gene 371:154-165(2006) [PubMed: 16481131] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB052638 mRNA. Translation: BAD83603.1. AY102608 mRNA. Translation: AAM28950.1. AB014765 mRNA. Translation: BAC66462.1. AF070523 mRNA. Translation: AAC64360.1. AF115965 mRNA. Translation: AAP97237.1. AF125530 mRNA. Translation: AAF17224.1. AF161476 mRNA. Translation: AAF29091.1. AB097051 mRNA. Translation: BAC77404.1. AK223566 mRNA. Translation: BAD97286.1. Different initiation. BC005143 mRNA. Translation: AAH05143.1. BC020797 mRNA. Translation: AAH20797.1.
RefSeq	NP_006398.1.
UniGene	Hs.518060 Hs.699963
3D structure databases
ModBase	Search...
Proteomic databases
PeptideAtlas	O75915.
Genome annotation databases
Ensembl	ENSG00000144746. Homo sapiens. [Contig view]
GeneID	10550.
KEGG	hsa:10550.
Organism-specific databases
HGNC	HGNC:16937. ARL6IP5.
HPA	HPA015540.
MIM	605709. gene.
PharmGKB	PA134898937.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O75915.
HOVERGEN	O75915.
Gene expression databases
ArrayExpress	O75915.
CleanEx	HS_ARL6IP5.
GermOnline	ENSG00000144746. Homo sapiens.
Family and domain databases
InterPro	IPR004895. Prenylated_rab_accept_PRA1. [Graphical view]
Pfam	PF03208. PRA1. 1 hit. [Graphical view]
ProDom	O75915. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

PRAF3_HUMAN

Accession

Primary (citable) accession number: O75915
Secondary accession number(s): Q53ES3, Q5KU08

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	November 1, 1998
Last modified:	July 22, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)