Reviewed, UniProtKB/Swiss-Prot O75952 (CABYR_HUMAN)

Names and origin

Protein names

Recommended name:
    Calcium-binding tyrosine phosphorylation-regulated protein
Alternative name(s):
    Calcium-binding protein 86
    Testis-specific calcium-binding protein CBP86
    Fibrousheathin-2
    FSP-2
    Fibrousheathin II
    Cancer/testis antigen 88
      Short name=CT88

Gene names

Name:	CABYR
Synonyms:	CBP86, FSP2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. Isoform 1 binds calcium in vitro. Isoform 2 and isoform 6 probably bind calcium. Isoform 3 and isoform 5 do not bind calcium in vitro. Isoform 4 probably does not bind calcium.
Subunit structure	Interacts with FSCB By similarity. Isoform 3 self-associates. Isoform 3 and isoform 5 interact with GSK3B. Isoform 1 does not interact with GSK3B.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection › cilium › flagellum. Note= Localized to fibrous sheath including the surface of the longitudinal columns and ribs of the principal piece of sperm flagella. Ref.1 Ref.4 Ref.5 Isoform 1: Nucleus. Cytoplasm. Cell projection › cilium › flagellum. Note= According to Ref.4, isoform 1, isoform 3 and isoform 5 are both nuclear and cytoplasmic. Ref.1 Ref.4 Ref.5 Isoform 3: Nucleus. Cytoplasm. Cell projection › cilium › flagellum. Note= According to Ref.4, isoform 1, isoform 3 and isoform 5 are both nuclear and cytoplasmic. Ref.1 Ref.4 Ref.5 Isoform 5: Nucleus. Cytoplasm. Cell projection › cilium › flagellum. Note= According to Ref.4, isoform 1, isoform 3 and isoform 5 are both nuclear and cytoplasmic. Ref.1 Ref.4 Ref.5
Tissue specificity	Expressed in elongating spermatids and spermatozoa (at protein level). Isoform 1 is expressed in testis. Isoform 3 and isoform 5 are also expressed in brain, pancreas and numerous brain tumors. Ref.1 Ref.4 Ref.5
Post-translational modification	Isoform 1 is phosphorylated on tyrosine residues during in vitro capacitation. Isoform 3 and isoform 5 are phosphorylated by GSK3B in vitro. Dephosphorylation affects its ability to bind calcium. Ref.1 Ref.4 Ref.3
Sequence similarities	Contains 1 RIIa domain.

Ontologies

Keywords
Cellular component	Cell projection Cilium Cytoplasm Cytoskeleton Flagellum Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Calcium Metal-binding
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cilium Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-KW flagellum Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cAMP-dependent protein kinase regulator activity Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75952-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: According to Ref.4, isoform 1, isoform 3 and isoform 5 are both nuclear and cytoplasmic. Ref.1 Ref.4 Ref.5

Isoform 2 (identifier: O75952-2)

Also known as: CBP86-VII,;

The sequence of this isoform differs from the canonical sequence as follows:
     49-66: Missing.

Isoform 3 (identifier: O75952-3)

Also known as: CBP86-II;

The sequence of this isoform differs from the canonical sequence as follows:
     181-379: GKVSSIHSDQ...ENAKYSSVYM → AMATSERGQP...HKRRKAETEN
     380-493: Missing.

Notes: According to Ref.4, isoform 1, isoform 3 and isoform 5 are both nuclear and cytoplasmic. Ref.1 Ref.4 Ref.5

Isoform 4 (identifier: O75952-4)

Also known as: CBP86-IV;

The sequence of this isoform differs from the canonical sequence as follows:
     181-221: GKVSSIHSDQ...SEAAEDVMVA → EDVAKKSSGS...HKRRKAETEN
     222-493: Missing.

Isoform 5 (identifier: O75952-5)

Also known as: CBP86-III;

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: Missing.
     181-379: GKVSSIHSDQ...ENAKYSSVYM → AMATSERGQP...HKRRKAETEN
     380-493: Missing.

Notes: According to Ref.4, isoform 1, isoform 3 and isoform 5 are both nuclear and cytoplasmic. Ref.1 Ref.4 Ref.5

Isoform 6 (identifier: O75952-6)

Also known as: CBP86-VI;

The sequence of this isoform differs from the canonical sequence as follows:
     1-201: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Calcium-binding tyrosine phosphorylation-regulated protein		PRO_0000089268
Regions
	Domain	12 – 49	38	RIIa
Amino acid modifications
	Modified residue	151	1	Phosphothreonine Probable
	Modified residue	155	1	Phosphoserine Probable
	Modified residue	240	1	Phosphoserine Ref.3
	Modified residue	359	1	Phosphoserine Ref.3
	Modified residue	362	1	Phosphoserine Ref.3
	Modified residue	413	1	Phosphoserine Ref.3
	Modified residue	461	1	Phosphoserine Ref.3
	Modified residue	469	1	Phosphoserine Ref.3
Natural variations
	Alternative sequence	1 – 201	201	Missing in isoform 6.		VSP_016245
	Alternative sequence	1 – 98	98	Missing in isoform 5.		VSP_016246
	Alternative sequence	49 – 66	18	Missing in isoform 2.		VSP_016247
	Alternative sequence	181 – 379	199	GKVSS…SSVYM → AMATSERGQPPPCSNMWTLY CLTDKNQQGHPSPPPAPGPF PQATLYLPNPKDPQFQQHPP KVTFPTYVMGDTKKTSAPPF ILVGSNVQEAQGWKPLPGHA VVSQSDVLRYVAMQVPIAVP ADEKYQKHTLSPQNANPPSG QDVPRPKSPVFLSVAFPVED VAKKSSGSGDKCAPFGSYGI AGEVTVTTAHKRRKAETEN in isoform 3 and isoform 5.		VSP_016248
	Alternative sequence	181 – 221	41	GKVSS…DVMVA → EDVAKKSSGSGDKCAPFGSY GIAGEVTVTTAHKRRKAETE N in isoform 4.		VSP_016249
	Alternative sequence	222 – 493	272	Missing in isoform 4.		VSP_016250
	Alternative sequence	380 – 493	114	Missing in isoform 3 and isoform 5.		VSP_016251
	Natural variant	74	1	T → M: dbSNP rs3786417.		VAR_030040
	Natural variant	186	1	I → V: dbSNP rs35118855.		VAR_050709
	Natural variant	448	1	K → R: dbSNP rs1049682. Ref.1		VAR_030041
	Natural variant	490	1	S → A: dbSNP rs1049683. Ref.1		VAR_023818
Experimental info
	Mutagenesis	146	1	T → A: Does not affect phosphorylation Ref.4
	Mutagenesis	151	1	T → A: Decreases phosphorylation. Abolishes phosphorylation; when associated with A-155 Ref.4
	Mutagenesis	154	1	S → A: Does not affect phosphorylation. Does not affect phosphorylation; when associated with A-159 Ref.4
	Mutagenesis	155	1	S → A: Decreases phosphorylation and interaction with GSK3B. Abolishes phosphorylation and decreases interaction with GSK3B; when associated with A-151 Ref.4
	Mutagenesis	159	1	T → A: Does not affect phosphorylation. Does not affect phosphorylation; when associated with A-154 Ref.4
	Sequence conflict	423	1	I → V in AAC35373 and AAL56051. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 42F97605262C8199	FASTA	493	52,774
	10 20 30 40 50 60 MISSKPRLVV PYGLKTLLEG ISRAVLKTNP SNINQFAAAY FQELTMYRGN TTMDIKDLVK 70 80 90 100 110 120 QFHQIKVEKW SEGTTPQKKL ECLKEPGKTS VESKVPTQME KSTDTDEDNV TRTEYSDKTT 130 140 150 160 170 180 QFPSVYAVPG TEQTEAVGGL SSKPATPKTT TPPSSPPPTA VSPEFAYVPA DPAQLAAQML 190 200 210 220 230 240 GKVSSIHSDQ SDVLMVDVAT SMPVVIKEVP SSEAAEDVMV AAPLVCSGKV LEVQVVNQTS 250 260 270 280 290 300 VHVDLGSQPK ENEAEPSTAS SVPLQDEQEP PAYDQAPEVT LQADIEVMST VHISSVYNDV 310 320 330 340 350 360 PVTEGVVYIE QLPEQIVIPF TDQVACLKEN EQSKENEQSP RVSPKSVVEK TTSGMSKKSV 370 380 390 400 410 420 ESVKLAQLEE NAKYSSVYME AEATALLSDT SLKGQPEVPA QLLDAEGAIK IGSEKSLHLE 430 440 450 460 470 480 VEITSIVSDN TGQEESGENS VPQEMEGKPV LSGEAAEAVH SGTSVKSSSG PFPPAPEGLT 490 APEIEPEGES TAE
	Isoform 2 (CBP86-VII,). Checksum: 01D34365BEDF945B		475	50,676
	10 20 30 40 50 60 MISSKPRLVV PYGLKTLLEG ISRAVLKTNP SNINQFAAAY FQELTMYRVE KWSEGTTPQK 70 80 90 100 110 120 KLECLKEPGK TSVESKVPTQ MEKSTDTDED NVTRTEYSDK TTQFPSVYAV PGTEQTEAVG 130 140 150 160 170 180 GLSSKPATPK TTTPPSSPPP TAVSPEFAYV PADPAQLAAQ MLGKVSSIHS DQSDVLMVDV 190 200 210 220 230 240 ATSMPVVIKE VPSSEAAEDV MVAAPLVCSG KVLEVQVVNQ TSVHVDLGSQ PKENEAEPST 250 260 270 280 290 300 ASSVPLQDEQ EPPAYDQAPE VTLQADIEVM STVHISSVYN DVPVTEGVVY IEQLPEQIVI 310 320 330 340 350 360 PFTDQVACLK ENEQSKENEQ SPRVSPKSVV EKTTSGMSKK SVESVKLAQL EENAKYSSVY 370 380 390 400 410 420 MEAEATALLS DTSLKGQPEV PAQLLDAEGA IKIGSEKSLH LEVEITSIVS DNTGQEESGE 430 440 450 460 470 NSVPQEMEGK PVLSGEAAEA VHSGTSVKSS SGPFPPAPEG LTAPEIEPEG ESTAE
	Isoform 3 (CBP86-II). Checksum: 9E6748129EEE16E4		379	41,120
	10 20 30 40 50 60 MISSKPRLVV PYGLKTLLEG ISRAVLKTNP SNINQFAAAY FQELTMYRGN TTMDIKDLVK 70 80 90 100 110 120 QFHQIKVEKW SEGTTPQKKL ECLKEPGKTS VESKVPTQME KSTDTDEDNV TRTEYSDKTT 130 140 150 160 170 180 QFPSVYAVPG TEQTEAVGGL SSKPATPKTT TPPSSPPPTA VSPEFAYVPA DPAQLAAQML 190 200 210 220 230 240 AMATSERGQP PPCSNMWTLY CLTDKNQQGH PSPPPAPGPF PQATLYLPNP KDPQFQQHPP 250 260 270 280 290 300 KVTFPTYVMG DTKKTSAPPF ILVGSNVQEA QGWKPLPGHA VVSQSDVLRY VAMQVPIAVP 310 320 330 340 350 360 ADEKYQKHTL SPQNANPPSG QDVPRPKSPV FLSVAFPVED VAKKSSGSGD KCAPFGSYGI 370 AGEVTVTTAH KRRKAETEN
	Isoform 4 (CBP86-IV). Checksum: 0C6FA78696F9BF11		221	23,964
	10 20 30 40 50 60 MISSKPRLVV PYGLKTLLEG ISRAVLKTNP SNINQFAAAY FQELTMYRGN TTMDIKDLVK 70 80 90 100 110 120 QFHQIKVEKW SEGTTPQKKL ECLKEPGKTS VESKVPTQME KSTDTDEDNV TRTEYSDKTT 130 140 150 160 170 180 QFPSVYAVPG TEQTEAVGGL SSKPATPKTT TPPSSPPPTA VSPEFAYVPA DPAQLAAQML 190 200 210 220 EDVAKKSSGS GDKCAPFGSY GIAGEVTVTT AHKRRKAETE N
	Isoform 5 (CBP86-III). Checksum: 93966975E7AAFF08		281	30,053
	10 20 30 40 50 60 MEKSTDTDED NVTRTEYSDK TTQFPSVYAV PGTEQTEAVG GLSSKPATPK TTTPPSSPPP 70 80 90 100 110 120 TAVSPEFAYV PADPAQLAAQ MLAMATSERG QPPPCSNMWT LYCLTDKNQQ GHPSPPPAPG 130 140 150 160 170 180 PFPQATLYLP NPKDPQFQQH PPKVTFPTYV MGDTKKTSAP PFILVGSNVQ EAQGWKPLPG 190 200 210 220 230 240 HAVVSQSDVL RYVAMQVPIA VPADEKYQKH TLSPQNANPP SGQDVPRPKS PVFLSVAFPV 250 260 270 280 EDVAKKSSGS GDKCAPFGSY GIAGEVTVTT AHKRRKAETE N
	Isoform 6 (CBP86-VI). Checksum: E371F64036F2AA37		292	30,936
	10 20 30 40 50 60 MPVVIKEVPS SEAAEDVMVA APLVCSGKVL EVQVVNQTSV HVDLGSQPKE NEAEPSTASS 70 80 90 100 110 120 VPLQDEQEPP AYDQAPEVTL QADIEVMSTV HISSVYNDVP VTEGVVYIEQ LPEQIVIPFT 130 140 150 160 170 180 DQVACLKENE QSKENEQSPR VSPKSVVEKT TSGMSKKSVE SVKLAQLEEN AKYSSVYMEA 190 200 210 220 230 240 EATALLSDTS LKGQPEVPAQ LLDAEGAIKI GSEKSLHLEV EITSIVSDNT GQEESGENSV 250 260 270 280 290 PQEMEGKPVL SGEAAEAVHS GTSVKSSSGP FPPAPEGLTA PEIEPEGEST AE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CABYR, a novel calcium-binding tyrosine phosphorylation-regulated fibrous sheath protein involved in capacitation." Naaby-Hansen S., Mandal A., Wolkowicz M.J., Sen B., Westbrook V.A., Shetty J., Coonrod S.A., Klotz K.L., Kim Y.-H., Bush L.A., Flickinger C.J., Herr J.C. Dev. Biol. 242:236-254(2002) [PubMed: 11820818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5 AND 6), PROTEIN SEQUENCE OF 8-23; 28-48; 379-388 AND 394-410, VARIANTS ARG-448 AND ALA-490, CALCIUM-BINDING, PHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Ovary.
[3]	"Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation." Ficarro S., Chertihin O., Westbrook V.A., White F., Jayes F., Kalab P., Marto J.A., Shabanowitz J., Herr J.C., Hunt D.F., Visconti P.E. J. Biol. Chem. 278:11579-11589(2003) [PubMed: 12509440] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240; SER-359; SER-362; SER-413; SER-461 AND SER-469, MASS SPECTROMETRY. Tissue: Sperm.
[4]	"Characterization of two non-testis-specific CABYR variants that bind to GSK3beta with a proline-rich extensin-like domain." Hsu H.-C., Lee Y.-L., Cheng T.-S., Howng S.-L., Chang L.-K., Lu P.-J., Hong Y.-R. Biochem. Biophys. Res. Commun. 329:1108-1117(2005) [PubMed: 15752768] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 3 AND 5), SUBUNIT, INTERACTION WITH GSK3B, PHOSPHORYLATION AT THR-151 AND SER-155, MUTAGENESIS OF THR-146; THR-151; SER-154; SER-155 AND THR-159, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Translation and assembly of CABYR coding region B in fibrous sheath and restriction of calcium binding to coding region A." Kim Y.-H., Jha K.N., Mandal A., Vanage G., Farris E., Snow P.L., Klotz K., Naaby-Hansen S., Flickinger C.J., Herr J.C. Dev. Biol. 286:46-56(2005) [PubMed: 16139264] [Abstract] Cited for: CALCIUM-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF088868 mRNA. Translation: AAC35373.1. AF295037 mRNA. Translation: AAG17889.1. AF295038 mRNA. Translation: AAG17890.1. AF295039 mRNA. Translation: AAG17891.1. AF329634 mRNA. Translation: AAL56051.1. AY007205 mRNA. Translation: AAG01891.1. BC011996 mRNA. Translation: AAH11996.1.
RefSeq	NP_036321.2. NP_619584.1. NP_619585.1. NP_722452.1. NP_722453.1. NP_722454.1.
UniGene	Hs.511983
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	O75952.
Genome annotation databases
Ensembl	ENSG00000154040. Homo sapiens. [Contig view]
GeneID	26256.
KEGG	hsa:26256.
Organism-specific databases
GeneCards	GC18P019972.
H-InvDB	HIX0014370.
HGNC	HGNC:15569. CABYR.
PharmGKB	PA26005.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75952.
HOVERGEN	O75952.
Gene expression databases
ArrayExpress	O75952.
CleanEx	HS_CABYR.
GermOnline	ENSG00000154040. Homo sapiens.
Family and domain databases
InterPro	IPR003117. cAMP-dep_prot_kin_reg_I/II_a/b. [Graphical view]
Pfam	PF02197. RIIa. 1 hit. [Graphical view]
SMART	SM00394. RIIa. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	48481.

Entry information

Entry name

CABYR_HUMAN

Accession

Primary (citable) accession number: O75952
Secondary accession number(s): Q8WXW5 , Q9HAY3, Q9HAY4, Q9HAY5, Q9HCY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	January 23, 2007
Last modified:	December 16, 2008
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)