Reviewed, UniProtKB/Swiss-Prot O75969 (AKAP3_HUMAN)

Names and origin

Protein names

Recommended name:
    A-kinase anchor protein 3
Alternative name(s):
    Protein kinase A-anchoring protein 3
      Short name=PRKA3
    A-kinase anchor protein 110 kDa
      Short name=AKAP 110
    Sperm oocyte-binding protein
    Fibrousheathin-1
    Fibrousheathin I
    Fibrous sheath protein of 95 kDa
      Short name=FSP95
    Cancer/testis antigen 82
      Short name=CT82

Gene names

Name:	AKAP3
Synonyms:	AKAP110, SOB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
Subunit structure	Interacts with ROPN1 AND ROPN1L. Ref.4
Subcellular location	AcrosomeBy similarity. Note= Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.
Tissue specificity	Testis specific; only expressed in spermatids.
Domain	RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Post-translational modification	Phosphorylated on tyrosine residues. Ref.5
Sequence similarities	Belongs to the AKAP110 family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	acrosome reaction Ref.3 Traceable author statement. Source: ProtInc cell motion Ref.3 Traceable author statement. Source: ProtInc
Cellular component	acrosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein kinase A binding Ref.3 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 853	853	A-kinase anchor protein 3		PRO_0000064526
Regions
	Region	124 – 137	14	PKA-RII subunit binding domain
Amino acid modifications
	Modified residue	205	1	Phosphoserine Ref.5
	Modified residue	208	1	Phosphoserine Ref.5
	Modified residue	403	1	Phosphoserine Ref.5
	Modified residue	404	1	Phosphotyrosine Ref.5
	Modified residue	440	1	Phosphoserine Ref.5
	Modified residue	612	1	Phosphoserine Ref.5
	Modified residue	635	1	Phosphoserine Ref.5
	Modified residue	636	1	Phosphoserine Ref.5
Natural variations
	Natural variant	831	1	R → C in a colorectal cancer sample; somatic mutation. Ref.6		VAR_036428
Experimental info
	Mutagenesis	131	1	L → P: Abolishes interaction with ROPN1 Ref.4
	Sequence conflict	118	1	G → E AA sequence Ref.2
	Sequence conflict	272	1	E → D in AAD21218. Ref.1
	Sequence conflict	282	1	S → G in AAC35854. Ref.2
	Sequence conflict	467	1	K → N in AAC35854. Ref.2
	Sequence conflict	500	1	I → T in AAC35854. Ref.2
	Sequence conflict	597	1	F → S AA sequence Ref.2
	Sequence conflict	700	1	S → L in AAC35854. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O75969-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 8374FFF1009BEBEC	FASTA	853	94,736
	10 20 30 40 50 60 MSEKVDWLQS QNGVCKVDVY SPGDNQAQDW KMDTSTDPVR VLSWLRRDLE KSTAEFQDVR 70 80 90 100 110 120 FKPGESFGGE TSNSGDPHKG FSVDYYNTTT KGTPERLHFE MTHKEIPCQG PRAQLGNGSS 130 140 150 160 170 180 VDEVSFYANR LTNLVIAMAR KEINEKIDGS ENKCVYQSLY MGNEPTPTKS LSKIASELVN 190 200 210 220 230 240 ETVSACSRNA APDKAPGSGD RVSGSSQSPP NLKYKSTLKI KESTKERQGP DDKPPSKKSF 250 260 270 280 290 300 FYKEVFESRN GDYAREGGRF FPRERKRFRG QERPDDFTAS VSEGIMTYAN SVVSDMMVSI 310 320 330 340 350 360 MKTLKIQVKD TTIATILLKK VLLKHAKEVV SDLIDSFLRN LHSVTGTLMT DTQFVSAVKR 370 380 390 400 410 420 TVFSHGSQKA TDIMDAMLRK LYNVMFAKKV PEHVRKAQDK AESYSLISMK GMGDPKNRNV 430 440 450 460 470 480 NFAMKSETKL REKMYSEPKS EEETCAKTLG EHIIKEGLTL WHKSQQKECK SLGFQHAAFE 490 500 510 520 530 540 APNTQRKPAS DISFEYPEDI GNLSLPPYPP EKPENFMYDS DSWAKDLIVS ALLLIQYHLA 550 560 570 580 590 600 QGGRRDARSF VEAAGTTNFP ANEPPVAPDE SCLKSAPIVG DQEQAEKKDL RSVFFNFIRN 610 620 630 640 650 660 LLSETIFKRD QSPEPKVPEQ PVKEDRKLCE RPLASSPPRL YEDDETPGAL SGLTKMAVSQ 670 680 690 700 710 720 IDGHMSGQMV EHLMNSVMKL CVIIAKSCDA SLAELGDDKS GDASRLTSAF PDSLYECLPA 730 740 750 760 770 780 KGTGSAEAVL QNAYQAIHNE MRGTSGQPPE GCAAPTVIVS NHNLTDTVQN KQLQAVLQWV 790 800 810 820 830 840 AASELNVPIL YFAGDDEGIQ EKLLQLSAAA VDKGCSVGEV LQSVLRYEKE RQLNEAVGNV 850 TPLQLLDWLM VNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition." Lefevre A., Duquenne C., Rousseau-Merck M.-F., Rogier E., Finaz C. Biochem. Biophys. Res. Commun. 259:60-66(1999) [PubMed: 10334916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Testis.
[2]	"FSP95, a testis-specific 95-kilodalton fibrous sheath antigen that undergoes tyrosine phosphorylation in capacitated human spermatozoa." Mandal A., Naaby-Hansen S., Wolkowicz M.J., Klotz K., Shetty J., Retief J.D., Coonrod S.A., Kinter M., Sherman N., Cesar F., Flickinger C.J., Herr J.C. Biol. Reprod. 61:1184-1197(1999) [PubMed: 10529264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Testis.
[3]	"Isolation and molecular characterization of AKAP110, a novel, sperm-specific protein kinase A-anchoring protein." Vijayaraghavan S., Liberty G.A., Mohan J., Winfrey V.P., Olson G.E., Carr D.W. Mol. Endocrinol. 13:705-717(1999) [PubMed: 10319321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[4]	"Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA." Carr D.W., Fujita A., Stentz C.L., Liberty G.A., Olson G.E., Narumiya S. J. Biol. Chem. 276:17332-17338(2001) [PubMed: 11278869] [Abstract] Cited for: INTERACTION WITH ROPN1 AND ROPN1L, MUTAGENESIS OF LEU-131.
[5]	"Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation." Ficarro S., Chertihin O., Westbrook V.A., White F., Jayes F., Kalab P., Marto J.A., Shabanowitz J., Herr J.C., Hunt D.F., Visconti P.E. J. Biol. Chem. 278:11579-11589(2003) [PubMed: 12509440] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205; SER-208; SER-403; TYR-404; SER-440; SER-612; SER-635 AND SER-636, MASS SPECTROMETRY. Tissue: Sperm.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-831.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U85715 mRNA. Translation: AAD21218.1. AF087003 mRNA. Translation: AAC35854.1. AF093408 mRNA. Translation: AAC63371.1.
RefSeq	NP_006413.2.
UniGene	Hs.98397
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	O75969.
Proteomic databases
PRIDE	O75969.
Genome annotation databases
Ensembl	ENSG00000111254. Homo sapiens. [Contig view]
GeneID	10566.
KEGG	hsa:10566.
Organism-specific databases
GeneCards	GC12M004594.
H-InvDB	HIX0026339.
HGNC	HGNC:373. AKAP3.
MIM	604689. gene.
PharmGKB	PA24667.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75969.
HOVERGEN	O75969.
Gene expression databases
ArrayExpress	O75969.
CleanEx	HS_AKAP3.
GermOnline	ENSG00000111254. Homo sapiens.
Family and domain databases
InterPro	IPR008382. AKAP_110. [Graphical view]
PANTHER	PTHR10226. AKAP_110. 1 hit.
Pfam	PF05716. AKAP_110. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40101.
SOURCE	Search...

Entry information

Entry name

AKAP3_HUMAN

Accession

Primary (citable) accession number: O75969
Secondary accession number(s): O75945, Q9UM61

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)