Reviewed, UniProtKB/Swiss-Prot O76217 (PE1_ANOGA)

Names and origin

Protein names

Recommended name:
    Peritrophin-1
Alternative name(s):
    Peritrophin A
    Peritrophic matrix protein 1
    AgAper-1

Gene names

Name:	Aper1
ORF Names:	AGAP006795

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds chitin but not cellulose. May be involved in the spatial organization of PM. Ref.1
Tissue specificity	Adult peritrophic membrane. Ref.1
Developmental stage	Expressed in adult but not larval guts, whole pupae or whole bodies minus gut.
Post-translational modification	Glycosylated.
Sequence similarities	Contains 2 chitin-binding type-2 domains.

Ontologies

Keywords
Domain	Repeat Signal
Ligand	Chitin-binding
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chitin metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	chitin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 153	136	Peritrophin-1		PRO_0000023612
Regions
	Domain	18 – 79	62	Chitin-binding type-2 1
	Domain	92 – 153	62	Chitin-binding type-2 2
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Disulfide bond	56 ↔ 69		By similarity
	Disulfide bond	130 ↔ 143		By similarity
Natural variations
	Natural variant	7	1	L → V in strain: 4aRR, PEST.
	Natural variant	19	1	D → V in strain: 4aRR, PEST.
	Natural variant	28	1	E → K in strain: L3-5.
	Natural variant	37	1	P → S in strain: L3-5.
	Natural variant	55	1	K → Q in strain: Yaounde Yd41.
	Natural variant	73	1	A → S in strain: Yaounde Yd21, Yaounde Yd35, Yaounde Yd41.
	Natural variant	88	1	A → V in strain: G3.
	Natural variant	89	1	P → S in strain: 4aRR, PEST.

Sequences

	Sequence		Length	Mass (Da)
	O76217-1 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 2207D5130F487338	FASTA	153	16,791
	10 20 30 40 50 60 MKVSASLVLL LAAAVLADDR CPPQDDPEQP PVLLAHPTDC DKFLICNHGT PVVSKCPPGL 70 80 90 100 110 120 LWNDSQKQCD YPAQAQCAPG VTPNTEPAPK PSPNCPPEYD PDHMVYIPHE TDCGKYYICD 130 140 150 PYGVELEQTC PSGLHWNPVV NYCDFPELAQ CEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A type I peritrophic matrix protein from the malaria vector Anopheles gambiae binds to chitin. Cloning, expression, and characterization." Shen Z., Jacobs-Lorena M. J. Biol. Chem. 273:17665-17670(1998) [PubMed: 9651363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: G3. Tissue: Midgut.
[2]	"Anopheles peritrophic matrix protein-1 (AgAper-1) regulatory sequences mediate transgenic protein accumulation and secretion into the midgut lumen upon blood ingestion." Abraham E.G., Donnelly-Doman M., Fujioka H., Ghosh A., Moreira L., Jacobs-Lorena M. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Intraspecific nucleotide variation in Anopheles gambiae: new insights into the biology of malaria vectors." Morlais I., Poncon N., Simard F., Cohuet A., Fontenille D. Am. J. Trop. Med. Hyg. 71:795-802(2004) [PubMed: 15642974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 4aRR, L3-5, L3-51, Yaounde Yd21, Yaounde Yd35 and Yaounde Yd41.
[4]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF030431 mRNA. Translation: AAC39127.1. AY750997 Genomic DNA. Translation: AAV31069.1. AY344823 Genomic DNA. Translation: AAR02434.1. AY344824 Genomic DNA. Translation: AAR02435.1. AY344825 Genomic DNA. Translation: AAR02436.1. AY344826 Genomic DNA. Translation: AAR02437.1. AY344827 Genomic DNA. Translation: AAR02438.1. AY344828 Genomic DNA. Translation: AAR02439.1. AAAB01008807 Genomic DNA. Translation: EAA04177.3.
RefSeq	XP_308952.3.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	AGAP006795. Anopheles gambiae. [Contig view]
GeneID	1270270.
KEGG	aga:AgaP_AGAP006795.
VectorBase	AGAP006795. Anopheles gambiae.
Family and domain databases
InterPro	IPR002557. Chitin-bd_peritrophin-A. [Graphical view]
Pfam	PF01607. CBM_14. 2 hits. [Graphical view]
SMART	SM00494. ChtBD2. 2 hits. [Graphical view]
PROSITE	PS50940. CHIT_BIND_II. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PE1_ANOGA

Accession

Primary (citable) accession number: O76217
Secondary accession number(s): Q6VAW1 , Q6VAW2, Q6VAW4, Q6VAW5, Q6VAW6, Q7QID7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	July 5, 2005
Last modified:	November 25, 2008
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)