Reviewed, UniProtKB/Swiss-Prot O76856 (CATD_DICDI)

Names and origin

Protein names

Recommended name:
    Cathepsin D
    EC=3.4.23.5
Alternative name(s):
    Ddp44

Gene names

Name:	ctsD
Synonyms:	CatD
ORF Names:	DDB_0215012

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protease that may act during cell growth and/or development.
Catalytic activity	Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.
Subunit structure	Monomer.
Subcellular location	Lysosome. Secreted.
Developmental stage	Expressed in both vegetative and differentiating cells, with a higher level in vegetative cells.
Post-translational modification	N-glycosylated on 2 out of the 3 potential sites. Glycans contain sulfated Mannose.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular function	cathepsin D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 48	30
	Chain	49 – 383	335	Cathepsin D
Sites
	Active site	81	1	By similarity
	Active site	268	1	By similarity
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Glycosylation	310	1	N-linked (GlcNAc...) Potential
	Disulfide bond	94 ↔ 101		By similarity
	Disulfide bond	259 ↔ 263		By similarity
	Disulfide bond	302 ↔ 339		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O76856-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: CC8DE423AEA1A280	FASTA	383	41,121
	10 20 30 40 50 60 MKLLILTLFL ATIVLAQALT VPLNFHQASR ESRRRVPQKW SNRLSALNAG TTIPISDFED 70 80 90 100 110 120 AQYYGAITIG TPGQAFKVVF DTGSSNLWIP SKKCPITVVA CDLHNKYNSG ASSTYVANGT 130 140 150 160 170 180 DFTIQYGSGA MSGFVSQDSV TVGSLTVKDQ LFAEATAEPG IAFDFAKFDG ILGLAFQSIS 190 200 210 220 230 240 VNSIPPVFYN MLSQGLVSST LFSFWLSRTP GANGGELSFG SIDNTKYTGD ITYVPLTNET 250 260 270 280 290 300 YWEFVMDDFA IDGQSAGFCG TTCHAICDSG TSLIAGPMAD ITALNEKLGA VILNGEGVFS 310 320 330 340 350 360 DCSVINTLPN VTITVAGREF VLTPKEYVLE VTEFGKTECL SGFMGIELNM GNFWILGDVF 370 380 ISAYYTVFDF GNKQVGFATA IQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Dictyostelium discoideum cathepsin D. Molecular cloning, gene disruption, endo-lysosomal localization and sugar modifications." Journet A., Chapel A., Jehan S., Adessi C., Freeze H., Klein G., Garin J. J. Cell Sci. 112:3833-3843(1999) [PubMed: 10523518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 49-56; 77-92; 103-116; 269-278; 325-336 AND 370-383, SUBUNIT, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, GLYCOSYLATION. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y16962 mRNA. Translation: CAA76563.1. AJ243946 Genomic DNA. Translation: CAB57223.1. AAFI02000031 Genomic DNA. Translation: EAL67644.1.
RefSeq	XP_641645.1.
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
ModBase	Search...
Genome annotation databases
GeneID	3392787.
KEGG	ddi:DDB_0215012.
Organism-specific databases
dictyBase	DDB0215012. ctsD.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. [Graphical view]
ProDom	O76856. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CATD_DICDI

Accession

Primary (citable) accession number: O76856
Secondary accession number(s): Q54WS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	November 1, 1998
Last modified:	July 22, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)