Unreviewed, UniProtKB/TrEMBL O83008 (O83008_SERMA)

Names and origin

Protein names

Submitted name:
    Chitinase A EMBL BAA31567.1

Gene names

Name:

chiA EMBL BAA31567.1

Organism

Serratia marcescens EMBL BAA31567.1

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. RuleBase RU004453V0

Ontologies

Keywords
Domain	Signal
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential EMBL BAA31567.1
	Chain	24 – 563	540	chitinase A EMBL BAA31567.1		PRO_5000049238

Sequences

	Sequence		Length	Mass (Da)
	O83008-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: CF0B130905AF6E8B	FASTA	563	61,111
	10 20 30 40 50 60 MRKFNKPLLA LLIGSTLCSA AQAAAPGKPT IAWGNTKFAI VEVDQAATAY NNLVKVKNAA 70 80 90 100 110 120 DVSVSWNLWN GDTGTTAKVL LNGKEAWSGP STGSSGTANF KVNKGGRYQM QVALCNADGC 130 140 150 160 170 180 TASDATEIVV ADTDGSHLAP LKEPLLEKNK PYKQNSGKVV GSYFVEWGVY GRNFTVDKIP 190 200 210 220 230 240 AQNLTHLLYG FIPICGGNGI NDSLKEIEGS FQALQRSCQG REDFKVSIHD PFAALQKAQK 250 260 270 280 290 300 GVTAWDDPYK GNFGQLMALK QAHPDLKILP SIGGWTLSDP FFFMGDKVKR DRFVGSVKEF 310 320 330 340 350 360 LQTWKFFDGV DIDWEFPGGK GANPNLGSPQ DGETYVLLMK ELRAMLDQLS VETGRKYELT 370 380 390 400 410 420 SAISAGKDKI DKVAYNVAQN SMDHIFLMSY DFYGAFDLKN LGHQTALNAP AWKPDTAYTT 430 440 450 460 470 480 VNGVNALLAQ GVKPGKIVVG TAMYGRGWTG VNGYQNNIPF TGTATGPVKG TWENRIVDYR 490 500 510 520 530 540 QIAGQFMSGE WQYTYDATAE APYVFKPSTG DLITFDDARS VQAKGKYVLD KQLGGLFSWE 550 560 IDADNGDILN SMNASLGNSA GVQ

References

[1]	"Genetic analysis of the chitinase system of Serratia marcescens 2170." Watanabe T., Kimura K., Sumiya T., Nikaidou N., Suzuki K., Suzuki M., Taiyoji M., Ferrer S., Regue M. J. Bacteriol. 179:7111-7117(1997) [PubMed: 9371460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 2170 EMBL BAA31567.1.
[2]	Suzuki K., Nikaidou N., Watanabe T. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 2170 EMBL BAA31567.1.
[3]	"High resolution structural analyses of mutant chitinase A complexes with substrates provide new insight into the mechanism of catalysis." Papanikolau Y., Prag G., Tavlas G., Vorgias C.E., Oppenheim A.B., Petratos K. Biochemistry 40:11338-11343(2001) [PubMed: 11560481] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 24-563.
[4]	"De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin." Papanikolau Y., Tavlas G., Vorgias C.E., Petratos K. Acta Crystallogr. D Biol. Crystallogr. 59:400-403(2003) [PubMed: 12554965] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 24-563.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB015996 Genomic DNA. Translation: BAA31567.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EDQ	X-ray	1.55	A	24-563	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR011583. Chitinase_II.
IPR013540. ChitinaseA_N.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_sub_cat.
IPR013783. Ig-like_fold.
IPR000601. PKD.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

Pfam

PF08329. ChitinaseA_N. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]

ProDom

PD000471. Chitinase_II. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00636. Glyco_18. 1 hit.
SM00089. PKD. 1 hit.
[Graphical view]

PROSITE

PS01095. CHITINASE_18. 1 hit.
[Graphical view]

Entry information

Entry name

O83008_SERMA

Accession

Primary (citable) accession number: O83008

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)