Reviewed, UniProtKB/Swiss-Prot O88788 (AG10B_RAT)

Names and origin

Protein names

Recommended name:
    Putative alpha-1,2-glucosyltransferase ALG10-B
      Short name=Alpha-2-glucosyltransferase ALG10-B
    EC=2.4.1.-
Alternative name(s):
    Asparagine-linked glycosylation protein 10 homolog B
    Potassium channel regulator 1

Gene names

Name:	Alg10b
Synonyms:	Kcr1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol. When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2. Ref.1 Ref.2
Subunit structure	Interacts with KCNH1 and KCNH2. Ref.1 Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein. Ref.2
Tissue specificity	Highly expressed in brain, skeletal muscle, uterus, small intestine and liver. Moderately expressed in lung and kidney. Weakly expressed in heart and stomach. Ref.1
Sequence similarities	Belongs to the ALG10 glucosyltransferase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Putative alpha-1,2-glucosyltransferase ALG10-B		PRO_0000215449
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Potential
	Topological domain	28 – 64	37	Extracellular Potential
	Transmembrane	65 – 85	21	Potential
	Topological domain	86 – 97	12	Cytoplasmic Potential
	Transmembrane	98 – 118	21	Potential
	Topological domain	119 – 126	8	Extracellular Potential
	Transmembrane	127 – 147	21	Potential
	Topological domain	148 – 150	3	Cytoplasmic Potential
	Transmembrane	151 – 171	21	Potential
	Topological domain	172 – 175	4	Extracellular Potential
	Transmembrane	176 – 196	21	Potential
	Topological domain	197 – 256	60	Cytoplasmic Potential
	Transmembrane	257 – 277	21	Potential
	Topological domain	278 – 283	6	Extracellular Potential
	Transmembrane	284 – 304	21	Potential
	Topological domain	305 – 317	13	Cytoplasmic Potential
	Transmembrane	318 – 338	21	Potential
	Topological domain	339 – 365	27	Extracellular Potential
	Transmembrane	366 – 386	21	Potential
	Topological domain	387 – 392	6	Cytoplasmic Potential
	Transmembrane	393 – 413	21	Potential
	Topological domain	414 – 436	23	Extracellular Potential
	Transmembrane	437 – 457	21	Potential
	Topological domain	458 – 473	16	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	O88788-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 0901C1DEA9C7B9E9	FASTA	474	55,574
	10 20 30 40 50 60 MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE GRFSLSQWDP 70 80 90 100 110 120 MITTLPGLYL VSVGVVKPAS WILGWSEHVV CSIGMLRFVN LLFSVGNFYL LYLLFRKIQP 130 140 150 160 170 180 RNKASSSIQR ILSTLTLAVF PTLYFFNFLY YTEAGSVFFT LFAYLMCLYG NHRTSALLGF 190 200 210 220 230 240 CGFMFRQTNI IWAAFCAGHI IAQKCSEAWK TELQKKKEER LPPAKGPLSE LRRVLQFLLM 250 260 270 280 290 300 YSMSLKNLSM LFLLTWPYML LLLAFFVFVV VNGGIVVGDR SSHEACLHFP QLFYFFSFTA 310 320 330 340 350 360 FFSFPHLLSP TKVKTFLSLV WKRRVQFSVI TLVSVFLVWK FTYVHKYLLA DNRHYTFYVW 370 380 390 400 410 420 KRVFQRHEIV KYLLVPAYMF AGWAVADSLK SKSIFWNLMF FVCLVASTVP QKLLEFRYFI 430 440 450 460 470 LPYIIYRLNM PLPPISRLVC ELGCYAVVNF LTFYIFLNKT FQWSDSHDIQ RFMW

References

[1]

"KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels." Hoshi N., Takahashi H., Shahidullah M., Yokoyama S., Higashida H. J. Biol. Chem. 273:23080-23085(1998) [PubMed: 9722534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH KCNH1, TISSUE SPECIFICITY. Strain: Wistar. Tissue: Cerebellum.

[2]

"The IKr drug response is modulated by KCR1 in transfected cardiac and noncardiac cell lines." Kupershmidt S., Yang I.C.-H., Hayashi K., Wei J., Chanthaphaychith S., Petersen C.I., Johns D.C., George A.L. Jr., Roden D.M., Balser J.R. FASEB J. 17:2263-2265(2003) [PubMed: 14525949] [Abstract] Cited for: FUNCTION, INTERACTION WITH KCNH2, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U78090 mRNA. Translation: AAC34249.1.
RefSeq	NP_620801.1.
UniGene	Rn.3635
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000014511. Rattus norvegicus. [Contig view]
GeneID	245960.
KEGG	rno:245960.
Organism-specific databases
RGD	708500. Alg10b.
Phylogenomic databases
HOVERGEN	O88788.
Family and domain databases
InterPro	IPR016900. Alpha1_2_glucosyltferase_Alg10. IPR007006. DIE2_ALG10. [Graphical view]
PANTHER	PTHR12989. DIE2_ALG10. 1 hit.
Pfam	PF04922. DIE2_ALG10. 1 hit. [Graphical view]
PIRSF	PIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	623217.

Entry information

Entry name

AG10B_RAT

Accession

Primary (citable) accession number: O88788

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)