Reviewed, UniProtKB/Swiss-Prot O88869 (RASF9_RAT)

Names and origin

Protein names

Recommended name:
    Ras association domain-containing protein 9
Alternative name(s):
    Peptidylglycine alpha-amidating monooxygenase COOH-terminal interactor
    PAM COOH-terminal interactor protein 1
      Short name=P-CIP1

Gene names

Name:	Rassf9
Synonyms:	Pamci, Pcip1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in regulating vesicuar trafficking in cells. Ref.1
Subunit structure	Interacts with PAM. Ref.1
Subcellular location	Endosome. Note= Accumulates on perinuclear endosomes. Ref.1
Tissue specificity	Testis, kidney, skeletal muscle, liver, lung, brain, heart, pituitary gland, adrenal gland and ovary. Ref.1
Sequence similarities	Contains 1 Ras-associating domain.

Ontologies

Keywords
Cellular component	Endosome
Domain	Coiled coil
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	endosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pam	P14925	1	EBI-1395057,EBI-1395008

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Ras association domain-containing protein 9		PRO_0000299454
Regions
	Domain	25 – 119	95	Ras-associating
	Coiled coil	195 – 291	97	Potential

Sequences

	Sequence		Length	Mass (Da)
	O88869-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 582AF0C52176E9B1	FASTA	435	49,545
	10 20 30 40 50 60 MAPFGRNLLK TRHKNRSPTK DMDPEEKEIV VWVCQEEKIV CGLTKRTTSI DVIQALLEEH 70 80 90 100 110 120 EATFGEKRFL LGKASDYCIV EKWRGSERAL PPLTRILKLW KAWGDEQPNM QFVLVKTDAF 130 140 150 160 170 180 LPVPLWRTAE TKLVQNNEKP WELSPANYMK TLPPDKQKRI VRKTFRKLAK IRQDTGSHDR 190 200 210 220 230 240 DNMECLVHLI ISQDHTIHQQ VQRMKELDME IEKCEAKIHL DRIGNDGADY VQEAYLMPRS 250 260 270 280 290 300 SEEEQKLDFQ SEDNQTLEDL NDGEGVSQLE EQLQYYRALI DKLSAEIERE VKGAGTDGSE 310 320 330 340 350 360 DMEGAAACEL ENSDLESVKC DLEKSMKAGL KIHSHLSGIQ REIKYSDSLL QMKAREYELL 370 380 390 400 410 420 AKEFSSLHIS SKDGCQGKEN RGKEAEASSS NGEIPPLTQR VFNTYTNDTD SDTGISSNHS 430 QDSETTLGDV LLLAT

References

[1]

"P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes." Chen L., Johnson R.C., Milgram S.L. J. Biol. Chem. 273:33524-33532(1998) [PubMed: 9837933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PAM, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Sprague-Dawley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF056208 mRNA. Translation: AAD03249.1.
RefSeq	NP_075248.1.
UniGene	Rn.30013
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O88869. 1 interaction.
Genome annotation databases
Ensembl	ENSRNOG00000038574. Rattus norvegicus. [Contig view]
GeneID	65053.
KEGG	rno:65053.
Organism-specific databases
RGD	621329. Rassf9.
Phylogenomic databases
HOVERGEN	O88869.
Gene expression databases
ArrayExpress	O88869.
Family and domain databases
InterPro	IPR000159. Ras-assoc. [Graphical view]
SMART	SM00314. RA. 1 hit. [Graphical view]
PROSITE	PS50200. RA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613907.

Entry information

Entry name

RASF9_RAT

Accession

Primary (citable) accession number: O88869

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)