Reviewed, UniProtKB/Swiss-Prot O89100 (GRAP2_MOUSE)

Names and origin

Protein names

Recommended name:
    GRB2-related adaptor protein 2
Alternative name(s):
    GADS protein
    Growth factor receptor-binding protein
    GRBLG
    GRB-2-like protein
      Short name=GRB2L
    Hematopoietic cell-associated adaptor protein GrpL
    GRB-2-related monocytic adapter protein
      Short name=Monocytic adapter
      Short name=MONA
    Adapter protein GRID

Gene names

Name:	Grap2
Synonyms:	Gads, Grb2l, Grid, Mona

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.
Subunit structure	Interacts with phosphorylated LAT and LAX1 upon TCR activation. Interacts with SHB By similarity. Interacts with phosphorylated LIME1 upon TCR activation.
Sequence similarities	Belongs to the GRB2/sem-5/DRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.

Ontologies

Keywords
Domain	Repeat SH2 domain SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 322

322

GRB2-related adaptor protein 2

PRO_0000088209

Regions

Domain

1 – 56

56

SH3 1

Domain

58 – 149

92

SH2

Domain

263 – 322

60

SH3 2

Amino acid modifications

Modified residue

45

1

Phosphotyrosine By similarity

Secondary structure

1

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322

Helix Strand Turn

Details...

Turn

54 – 58

5

Helix

65 – 73

9

Beta strand

79 – 84

6

Beta strand

86 – 88

3

Beta strand

92 – 97

6

Beta strand

99 – 106

8

Beta strand

115 – 119

5

Beta strand

121 – 124

4

Helix

125 – 132

8

Beta strand

137 – 141

5

Beta strand

267 – 270

4

Beta strand

289 – 294

6

Beta strand

297 – 305

9

Beta strand

308 – 313

6

Helix

314 – 316

3

Sequences

	Sequence		Length	Mass (Da)
	O89100-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 736311D0640CD3D0	FASTA	322	36,810
	10 20 30 40 50 60 MEATAKFDFM ASGEDELSFR TGDILKILSN QEEWLKAELG SQEGYVPKNF IDIEFPEWFH 70 80 90 100 110 120 EGLSRHQAEN LLMGKDIGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD TKGNYFLWTE 130 140 150 160 170 180 KFPSLNKLVD YYRTTSISKQ KQVFLRDGTQ DQGHRGNSLD RRSQGGPHPS GTVGEEIRPS 190 200 210 220 230 240 VNRKLSDHLP LGPQQFHPHQ QPSPQFTPGP QPPQQQRYLQ HFHQDRRGGS LDINDGHCGL 250 260 270 280 290 300 GSEVNATLMH RRHTDPVQLQ AAGRVRWARA LYDFEALEED ELGFRSGEVV EVLDSSNPSW 310 320 WTGRLHNKLG LFPANYVAPM MR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mona, a novel hematopoietic-specific adaptor interacting with the macrophage colony-stimulating factor receptor, is implicated in monocyte/macrophage development." Bourette R.P., Arnaud S., Myles G.M., Blanchet J.P., Rohrschneider L.R., Mouchiroud G. EMBO J. 17:7273-7281(1998) [PubMed: 9857184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc." Liu S.K., McGlade C.J. Oncogene 17:3073-3082(1998) [PubMed: 9872323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation." Law C.-L., Ewings M.K., Chaudhary P.M., Solow S.A., Yun T.J., Marshall A.J., Hood L., Clark E.A. J. Exp. Med. 189:1243-1253(1999) [PubMed: 10209041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28." Ellis J.H., Ashman C., Burden M.N., Kilpatrick K.E., Morse M.A., Hamblin P.A. J. Immunol. 164:5805-5814(2000) [PubMed: 10820259] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cloning of the human and mouse growth factor receptor binding protein like genes." Kedra D., Dumanski J.P. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[7]	"LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation." Hur E.M., Son M., Lee O.-H., Choi Y.B., Park C., Lee H., Yun Y. J. Exp. Med. 198:1463-1473(2003) [PubMed: 14610044] [Abstract] Cited for: INTERACTION WITH LIME1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF055465 mRNA. Translation: AAD08803.1.
AF053405 mRNA. Translation: AAC98669.1.
AF129477 mRNA. Translation: AAD41783.1.
AF236118 mRNA. Translation: AAF60318.1.
AJ011735 mRNA. Translation: CAA09756.1.
BC052496 mRNA. Translation: AAH52496.1.

RefSeq

NP_034945.1.

UniGene

Mm.12947

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H3H	NMR	-	A	263-322	[»]
1OEB	X-ray	1.76	A/B	263-322	[»]
1R1P	X-ray	1.80	A/B/C/D	50-147	[»]
1R1Q	X-ray	1.80	A/B	50-147	[»]
1R1S	X-ray	1.90	A/C/E/G	50-147	[»]
1UTI	X-ray	1.50	A	265-322	[»]
2D0N	X-ray	1.57	A/C	267-322	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O89100. 1 interaction.

PTM databases

PhosphoSite

O89100.

Genome annotation databases

Ensembl

ENSMUSG00000042351. Mus musculus. [Contig view]

GeneID

17444.

KEGG

mmu:17444.

Organism-specific databases

MGI

MGI:1333842. Grap2.

Phylogenomic databases

HOGENOM

O89100.

HOVERGEN

O89100.

Gene expression databases

ArrayExpress

O89100.

CleanEx

MM_GRAP2.

GermOnline

ENSMUSG00000042351. Mus musculus.

Family and domain databases

InterPro

IPR000980. SH2.
IPR001452. SH3.
[Graphical view]

Gene3D

G3DSA:3.30.505.10. SH2. 1 hit.

Pfam

PF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.

ProDom

PD000093. SH2. 1 hit.
PD000066. SH3. 2 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]

PROSITE

PS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

292080.

SOURCE

Search...

Entry information

Entry name

GRAP2_MOUSE

Accession

Primary (citable) accession number: O89100

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	December 16, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)