Reviewed, UniProtKB/Swiss-Prot O89752 (PA_I97A1)

Names and origin

Protein names

Recommended name:
    Polymerase acidic protein
Alternative name(s):
    RNA-directed RNA polymerase subunit P2

Gene names

Name:

PA

Organism

Influenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd) [Complete proteome]

Taxonomic identifier

130763 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Felis silvestris catus (Cat) [TaxID: 9685]
Panthera pardus (Leopard) [TaxID: 9691]
Panthera tigris (Tiger) [TaxID: 9694]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Post-translational modification	Phosphorylated on serines and threonines by host kinases, including human casein kinase II By similarity.
Sequence similarities	Belongs to the influenza viruses PA family.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Polymerase acidic protein		PRO_0000078786
Experimental info
	Sequence conflict	142	1	E → K in AAF74317. Ref.4
	Sequence conflict	448	1	A → G in AAC34270. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O89752-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 1F90020B425BF585	FASTA	716	82,749
	10 20 30 40 50 60 MEDFVRQCFN PMIVELAEKT MKEYGEDPKI ETNKFAAICT HLEVCFMYSD FHFIDERGES 70 80 90 100 110 120 IIVESGDPNA LLKHRFEIIE GRDRAMAWTV VNSICNTTGV DKPKFLPDLY DYKENRFTEI 130 140 150 160 170 180 GVTRREVHIY YLEKANKIKS EETHIHIFSF TGEEMATKAD YTLDEESRAR IKTRLFTIRQ 190 200 210 220 230 240 EMASRGLWDS FRQSERGEET IEERFEITGT MRRLADQSLP PNFSSLENFR AYVDGFKPNG 250 260 270 280 290 300 CIEGKLSQMS KEVNARIEPF LKTTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE 310 320 330 340 350 360 GIPLYDAIKC MKTFFGWREP NIIKPHEKGI NPNYLLAWKQ VLAELQDIEN EDKIPKTKNM 370 380 390 400 410 420 KKTSQLMWAL GENMAPEKVD FEDCKDIDDL KQYHSDEPEL RSLASWIQNE FNKACELTDS 430 440 450 460 470 480 SWIELDEIGE DVAPIEHIAS MRRNYFTAEV SHCRATEYIM KGVYINTALL NASCAAMDDF 490 500 510 520 530 540 QLIPMISKCR TKEGRRRTNL YGFIVKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY 550 560 570 580 590 600 CVLEIGEMLL RTAIGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES 610 620 630 640 650 660 SIKEKDMTKE FFENRSETWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY SSPQLEGFSA 670 680 690 700 710 ESRKLLLIVQ ALRDNLEPGT FDLEGLYGAI EECLINDPWV LLNASWFNSF LTHALR

References

[1]	"Comparisons of highly virulent H5N1 influenza A viruses isolated from humans and chickens from Hong Kong." Suarez D.L., Perdue M.L., Cox N., Rowe T., Bender C., Huang J., Swayne D.E. J. Virol. 72:6678-6688(1998) [PubMed: 9658115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between H9N2 and H5N1 human isolates." Lin Y.P., Shaw M., Gregory V., Cameron K., Lim W., Klimov A., Subbarao K., Guan Y., Krauss S., Shortridge K., Webster R., Cox N., Hay A. Proc. Natl. Acad. Sci. U.S.A. 97:9654-9658(2000) [PubMed: 10920197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Evolutionary characterization of the six internal genes of H5N1 human influenza A virus." Hiromoto Y., Yamazaki Y., Fukushima T., Saito T., Lindstrom S.E., Omoe K., Nerome R., Lim W., Sugita S., Nerome K. J. Gen. Virol. 81:1293-1303(2000) [PubMed: 10769072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[4]	"Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness." Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M., Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X., Fukuda K., Cox N. Science 279:393-396(1998) [PubMed: 9430591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-577.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF046095 Genomic RNA. Translation: AAC32097.1. AJ289874 Genomic RNA. Translation: CAB95840.1. AF084267 Genomic RNA. Translation: AAF74317.1. AF036361 Genomic RNA. Translation: AAC34270.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S62.001.
Family and domain databases
InterPro	IPR001009. RNA_pol_P2. [Graphical view]
Pfam	PF00603. Flu_PA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA_I97A1

Accession

Primary (citable) accession number: O89752
Secondary accession number(s): O56265, Q779I2, Q77VG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 1, 1998
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)