Reviewed, UniProtKB/Swiss-Prot O94510 (AGN2_SCHPO)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-alpha-glucosidase agn2
    EC=3.2.1.59
Alternative name(s):
    Endo-1,3-alpha-glucanase agn2

Gene names

Name:	agn2
ORF Names:	SPBC646.06c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the endolysis of the ascus wall.
Catalytic activity	Endohydrolysis of (1->3)-alpha-D-glucosidic linkages in isolichenin, pseudonigeran and nigeran.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 71 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW metabolic process Inferred from electronic annotation. Source: UniProtKB-KW spore germination Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cell surface Ref.1 Inferred from direct assay. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe fungal-type spore wall Ref.1 Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	glucan endo-1,3-alpha-glucosidase activity Ref.1 Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Glucan endo-1,3-alpha-glucosidase agn2		PRO_0000119035

Sequences

	Sequence		Length	Mass (Da)
	O94510-1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 7FA86E53523BC675	FASTA	433	48,066
	10 20 30 40 50 60 MASLTTALPN KAVVAHFMMG LTYNYAQSDF QNDIQNAISL GLDGFVLNFG NDSWMMSKLT 70 80 90 100 110 120 LMYNAADALN LQFLLYLNLD MSEMSTVPAS TLVTYVQTFA NRGHQARINN NVVVGTFLGQ 130 140 150 160 170 180 DINFGQSSVN QGWQVAFKNA LASAGINIFF MPTWPLDAST IYQTYPVADG FCKWNCWPYY 190 200 210 220 230 240 TSSPTSDAED LVYIQNSKAT NKKYMATVSP IFYTHFTSKN YSFFSEGLWF TRWMQLIKDQ 250 260 270 280 290 300 PNYVQVLTWN DYGESTYIGP TNYAADFPVI GSNSHEWVDS FTHAPLSYSL PLFIQMYKQN 310 320 330 340 350 360 TTGLPSNFSG ISQLYVTYRV HSKNATASSD SIPRPDNYQN SSDVISVISF AKSSYTLRVS 370 380 390 400 410 420 VNGTVLGTTN VNAGVQSANV SFIVNNTAAA GLPLFQILNG TTVIAQGYGP LNILGNNSVV 430 LYNFNFCTTR ISW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Role of the alpha-glucanase Agn1p in fission-yeast cell separation." Dekker N., Speijer D., Grun C.H., Van Den Berg M., De Haan A., Hochstenbach F. Mol. Biol. Cell 15:3903-3914(2004) [PubMed: 15194814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ND080.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY626902 mRNA. Translation: AAT84065.1. CU329671 Genomic DNA. Translation: CAH58744.1.
PIR	T40582.
RefSeq	XP_001713124.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2541122.
KEGG	spo:SPBC646.06c.
Organism-specific databases
GeneDB_Spombe	SPBC646.06c.
Gene expression databases
ArrayExpress	O94510.
Family and domain databases
InterPro	IPR005197. Glyco_hydro_71. [Graphical view]
Pfam	PF03659. Glyco_hydro_71. 1 hit. [Graphical view]
ProDom	PD139156. Glyco_hydro_71. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

AGN2_SCHPO

Accession

Primary (citable) accession number: O94510
Secondary accession number(s): Q5ZEQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)