Reviewed, UniProtKB/Swiss-Prot O94534 (ALP14_SCHPO)

Names and origin

Protein names

Recommended name:
    Spindle pole body component alp14
Alternative name(s):
    Altered polarity protein 14

Gene names

Name:	alp14
Synonyms:	mtc1
ORF Names:	SPCC895.07

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for bipolar spindle formation and proper chromosome segregation. Has a role in connecting the kinetochores and the plus end of pole to chromosome microtubules. Also required for the activation of the spindle checkpoint pathway.
Subunit structure	Interacts with alp14.
Subcellular location	Nucleus › spindle pole body. Kinetochore. Note= Kinetochore periphery. Spindle localization is alp7-dependent.
Sequence similarities	Belongs to the TOG/XMAP215 family. Contains 2 HEAT repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Kinetochore Microtubule Nucleus
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	attachment of spindle microtubules to kinetochore Ref.1 Inferred from direct assay. Source: GeneDB_SPombe cell division Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic microtubule organization Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: GeneDB_SPombe mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic cell cycle spindle assembly checkpoint Ref.1 Non-traceable author statement. Source: GeneDB_SPombe spindle assembly Ref.3 Traceable author statement. Source: GeneDB_SPombe
Cellular component	condensed chromosome kinetochore Ref.1 Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Ref.4 Inferred from direct assay. Source: GeneDB_SPombe microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	microtubule binding Traceable author statement. Source: GeneDB_SPombe
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
alp7	Q9URY2	1	EBI-1556727,EBI-1556697

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 809	809	Spindle pole body component alp14		PRO_0000064566
Regions
	Repeat	127 – 164	38	HEAT 1
	Repeat	167 – 204	38	HEAT 2
	Coiled coil	637 – 697	61	Potential
Amino acid modifications
	Modified residue	543	1	Phosphoserine
	Modified residue	548	1	Phosphoserine
	Modified residue	697	1	Phosphoserine
	Modified residue	720	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	O94534-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 78B2027F0EC9A939	FASTA	809	89,446
	10 20 30 40 50 60 MSQDQEEDYS KLPLESRIVH KVWKVRLSAY EECSKSFSLS ADGSDNCFEL WNNQSELWKS 70 80 90 100 110 120 VLTDSNVAAQ EAGTAAFVAY CRFSDPSHLL KAREISVLSI SEKCLTSPRA GTRENALEAL 130 140 150 160 170 180 MLLVEADSAA PVIESIIPSL SARSPKVIAS NVAAIASLVE QFGAKVIPSK MIIPHISNLF 190 200 210 220 230 240 GHADKNVRKE ASRLTVNIYR WTGDPLKDLL FKDLRPVQTK ELESLFAELP TEPPKQTRFL 250 260 270 280 290 300 KSQQPTSEPN VETQVEEQPA LENEESEPEP SDDQFDLVEE VDVLPNVDPN LETLMASSKW 310 320 330 340 350 360 KDRKEALDKL LPVLSQPKIK DNDFFNLVAI LTKSVSKDAN IMVVINAAHC IQAMAKGLRS 370 380 390 400 410 420 NFSKYASTSI NALLERSKEK KANVIESLSS AMDAVLATSS LDDLAELIAS FAGNKNPQIK 430 440 450 460 470 480 SSCFSLFSRS FSNMTSLPSK FTVDTCAKAC VPGVSDTFEP VRSAAAEALG VLMKLVGERA 490 500 510 520 530 540 INQYLSPLDD IRKSKIRSFY ETATVKAKAP TKKSKVKPSK QEESKVVVPS NAKAVKKSVV 550 560 570 580 590 600 PSSPVVPSPR KATNKSLSMD VSKGNAFENG PLLPRPTTRP VSRGLSRGTS SSLQQKVKAS 610 620 630 640 650 660 TPLNSGALNE TVQNLKNMEL DDPAPQPAKH SRVDRYEHPK VLEDNDSTIS SLESLKRENE 670 680 690 700 710 720 ELREQLKVEH EENISMQKQL SELKGELNTL RSARKASPIG DRKPAFMRRA NTDFLELSTS 730 740 750 760 770 780 PSFQRSVREF EPTRPKLYSS IDVNQRSPLA SAKTNGNFTF HAELPRSPFS SRANNINPDW 790 800 TKAIDLAAKL KQKITEMKQT DQRHQGLIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint." Garcia M.A., Vardy L., Koonrugsa N., Toda T. EMBO J. 20:3389-3401(2001) [PubMed: 11432827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation." Sato M., Vardy L., Angel Garcia M., Koonrugsa N., Toda T. Mol. Biol. Cell 15:1609-1622(2004) [PubMed: 14742702] [Abstract] Cited for: FUNCTION, INTERACTION WITH ALP7, SUBCELLULAR LOCATION.
[4]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543; SER-548; SER-697 AND SER-720, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB032409 Genomic DNA. Translation: BAA84527.1. CU329672 Genomic DNA. Translation: CAA22843.1.
PIR	T41645.
RefSeq	NP_588048.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O94534.
Genome annotation databases
GeneID	2539497.
KEGG	spo:SPCC895.07.
NMPDR	fig|4896.1.peg.386.
Organism-specific databases
GeneDB_Spombe	SPCC895.07.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002372-MON.
Gene expression databases
ArrayExpress	O94534.
Family and domain databases
InterPro	IPR000357. HEAT. [Graphical view]
Pfam	PF02985. HEAT. 2 hits. [Graphical view]
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALP14_SCHPO

Accession

Primary (citable) accession number: O94534

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)