Reviewed, UniProtKB/Swiss-Prot O94823 (AT10B_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable phospholipid-transporting ATPase VB
    EC=3.6.3.1

Gene names

Name:	ATP10B
Synonyms:	ATPVB, KIAA0715

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + H(2)O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Found in brain and in low levels in testis.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	phospholipid transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: O94823-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: O94823-2)

The sequence of this isoform differs from the canonical sequence as follows:
     461-529: AKRLETPKEL...RQRSMGHRES → GIEAPKGSIP...TCKLSFVYKG
     530-1461: Missing.

Notes: No experimental confirmation available.

Isoform C (identifier: O94823-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: MALSVDSSWH...INCSNIRIYE → MKKEGRKRWK...INNRECLIYS
     461-529: AKRLETPKEL...RQRSMGHRES → GIEAPKGSIP...TCKLSFVYKG
     530-1461: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1461	1461	Probable phospholipid-transporting ATPase VB		PRO_0000046381
Regions
	Topological domain	1 – 82	82	Cytoplasmic Potential
	Transmembrane	83 – 104	22	Potential
	Topological domain	105 – 110	6	Extracellular Potential
	Transmembrane	111 – 132	22	Potential
	Topological domain	133 – 316	184	Cytoplasmic Potential
	Transmembrane	317 – 338	22	Potential
	Topological domain	339 – 368	30	Extracellular Potential
	Transmembrane	369 – 390	22	Potential
	Topological domain	391 – 1111	721	Cytoplasmic Potential
	Transmembrane	1112 – 1132	21	Potential
	Topological domain	1133 – 1144	12	Extracellular Potential
	Transmembrane	1145 – 1164	20	Potential
	Topological domain	1165 – 1194	30	Cytoplasmic Potential
	Transmembrane	1195 – 1216	22	Potential
	Topological domain	1217 – 1223	7	Extracellular Potential
	Transmembrane	1224 – 1246	23	Potential
	Topological domain	1247 – 1252	6	Cytoplasmic Potential
	Transmembrane	1253 – 1273	21	Potential
	Topological domain	1274 – 1291	18	Extracellular Potential
	Transmembrane	1292 – 1316	25	Potential
	Topological domain	1317 – 1461	145	Cytoplasmic Potential
Sites
	Active site	433	1	4-aspartylphosphate intermediate By similarity
	Metal binding	1055	1	Magnesium By similarity
	Metal binding	1059	1	Magnesium By similarity
Natural variations
	Alternative sequence	1 – 156	156	MALSV…IRIYE → MKKEGRKRWKRKEDKKRVVV SNLLFEGWSHKENPNRHHRG NQIKTSKYTVLSFVPKNIFE QLHRFANLYFVGIAVLNFIP VVNAFQPEVSMIPICVILAV TAIKDAWEDLRRYKSDKVIN NRECLIYS in isoform C.		VSP_007305
	Alternative sequence	461 – 529	69	AKRLE…GHRES → GIEAPKGSIPLSKRKYPALL RNEEIKDILLALLEAVWHFH KLLPVSLWSSLSQIRAVPIT CKLSFVYKG in isoform B and isoform C.		VSP_007306
	Alternative sequence	530 – 1461	932	Missing in isoform B and isoform C.		VSP_007307
Experimental info
	Sequence conflict	217	1	C → R in BAB15074. Ref.3
	Sequence conflict	234	1	F → S in BAC03528. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 2676B90416B6B541	FASTA	1,461	165,391
	10 20 30 40 50 60 MALSVDSSWH RWQWRVRDGF PHCPSETTPL LSPEKGRQSY NLTQQRVVFP NNSIFHQDWE 70 80 90 100 110 120 EVSRRYPGNR TCTTKYTLFT FLPRNLFEQF HRWANLYFLF LVILNWMPSM EVFHREITML 130 140 150 160 170 180 PLAIVLFVIM IKDGMEDFKR HRFDKAINCS NIRIYERKEQ TYVQKCWKDV RVGDFIQMKC 190 200 210 220 230 240 NEIVPADILL LFSSDPNGIC HLETASLDGE TNLKQRCVVK GFSQQEVQFE PELFHNTIVC 250 260 270 280 290 300 EKPNNHLNKF KGYMEHPDQT RTGFGCESLL LRGCTIRNTE MAVGIVIYAG HETKAMLNNS 310 320 330 340 350 360 GPRYKRSKIE RRMNIDIFFC IGILILMCLI GAVGHSIWNG TFEEHPPFDV PDANGSFLPS 370 380 390 400 410 420 ALGGFYMFLT MIILLQVLIP ISLYVSIELV KLGQVFFLSN DLDLYDEETD LSIQCRALNI 430 440 450 460 470 480 AEDLGQIQYI FSDKTGTLTE NKMVFRRCTI MGSEYSHQEN AKRLETPKEL DSDGEEWTQY 490 500 510 520 530 540 QCLSFSARWA QDPATMRSQK GAQPLRRSQS ARVPIQGHYR QRSMGHRESS QPPVAFSSSI 550 560 570 580 590 600 EKDVTPDKNL LTKVRDAALW LETLSDSRPA KASLSTTSSI ADFFLALTIC NSVMVSTTTE 610 620 630 640 650 660 PRQRVTIKPS SKALGTSLEK IQQLFQKLKL LSLSQSFSST APSDTDLGES LGANVATTDS 670 680 690 700 710 720 DERDDASVCS GGDSTDDGGY RSSMWDQGDI LESGSGTSLE EALEAPATDL ARPEFCYEAE 730 740 750 760 770 780 SPDEAALVHA AHAYSFTLVS RTPEQVTVRL PQGTCLTFSL LCTLGFDSVR KRMSVVVRHP 790 800 810 820 830 840 LTGEIVVYTK GADSVIMDLL EDPACVPDIN MEKKLRKIRA RTQKHLDLYA RDGLRTLCIA 850 860 870 880 890 900 KKVVSEEDFR RWASFRREAE ASLDNRDELL METAQHLENQ LTLLGATGIE DRLQEGVPDT 910 920 930 940 950 960 IATLREAGIQ LWVLTGDKQE TAVNIAHSCR LLNQTDTVYT INTENQETCE SILNCALEEL 970 980 990 1000 1010 1020 KQFRELQKPD RKLFGFRLPS KTPSITSEAV VPEAGLVIDG KTLNAIFQGK LEKKFLELTQ 1030 1040 1050 1060 1070 1080 YCRSVLCCRS TPLQKSMIVK LVRDKLRVMT LSIGDGANDV SMIQAADIGI GISGQEGMQA 1090 1100 1110 1120 1130 1140 VMSSDFAITR FKHLKKLLLV HGHWCYSRLA RMVVYYLYKN VCYVNLLFWY QFFCGFSSST 1150 1160 1170 1180 1190 1200 MIDYWQMIFF NLFFTSLPPL VFGVLDKDIS AETLLALPEL YKSGQNSECY NLSTFWISMV 1210 1220 1230 1240 1250 1260 DAFYQSLICF FIPYLAYKGS DIDVFTFGTP INTISLTTIL LHQAMEMKTW TIFHGVVLLG 1270 1280 1290 1300 1310 1320 SFLMYFLVSL LYNATCVICN SPTNPYWVME GQLSNPTFYL VCFLTPVVAL LPRYFFLSLQ 1330 1340 1350 1360 1370 1380 GTCGKSLISK AQKIDKLPPD KRNLEIQSWR SRQRPAPVPE VARPTHHPVS SITGQDFSAS 1390 1400 1410 1420 1430 1440 TPKSSNPPKR KHVEESVLHE QRCGTECMRD DSCSGDSSAQ LSSGEHLLGP NRIMAYSRGQ 1450 1460 TDMCRCSKRS SHRRSQSSLT I
	Isoform B [UniParc]. Checksum: 048EAC8E3D5BBA0D		529	61,243
	10 20 30 40 50 60 MALSVDSSWH RWQWRVRDGF PHCPSETTPL LSPEKGRQSY NLTQQRVVFP NNSIFHQDWE 70 80 90 100 110 120 EVSRRYPGNR TCTTKYTLFT FLPRNLFEQF HRWANLYFLF LVILNWMPSM EVFHREITML 130 140 150 160 170 180 PLAIVLFVIM IKDGMEDFKR HRFDKAINCS NIRIYERKEQ TYVQKCWKDV RVGDFIQMKC 190 200 210 220 230 240 NEIVPADILL LFSSDPNGIC HLETASLDGE TNLKQRCVVK GFSQQEVQFE PELFHNTIVC 250 260 270 280 290 300 EKPNNHLNKF KGYMEHPDQT RTGFGCESLL LRGCTIRNTE MAVGIVIYAG HETKAMLNNS 310 320 330 340 350 360 GPRYKRSKIE RRMNIDIFFC IGILILMCLI GAVGHSIWNG TFEEHPPFDV PDANGSFLPS 370 380 390 400 410 420 ALGGFYMFLT MIILLQVLIP ISLYVSIELV KLGQVFFLSN DLDLYDEETD LSIQCRALNI 430 440 450 460 470 480 AEDLGQIQYI FSDKTGTLTE NKMVFRRCTI MGSEYSHQEN GIEAPKGSIP LSKRKYPALL 490 500 510 520 RNEEIKDILL ALLEAVWHFH KLLPVSLWSS LSQIRAVPIT CKLSFVYKG
	Isoform C [UniParc]. Checksum: D12B13EC2EC2E3D5		501	57,465
	10 20 30 40 50 60 MKKEGRKRWK RKEDKKRVVV SNLLFEGWSH KENPNRHHRG NQIKTSKYTV LSFVPKNIFE 70 80 90 100 110 120 QLHRFANLYF VGIAVLNFIP VVNAFQPEVS MIPICVILAV TAIKDAWEDL RRYKSDKVIN 130 140 150 160 170 180 NRECLIYSRK EQTYVQKCWK DVRVGDFIQM KCNEIVPADI LLLFSSDPNG ICHLETASLD 190 200 210 220 230 240 GETNLKQRCV VKGFSQQEVQ FEPELFHNTI VCEKPNNHLN KFKGYMEHPD QTRTGFGCES 250 260 270 280 290 300 LLLRGCTIRN TEMAVGIVIY AGHETKAMLN NSGPRYKRSK IERRMNIDIF FCIGILILMC 310 320 330 340 350 360 LIGAVGHSIW NGTFEEHPPF DVPDANGSFL PSALGGFYMF LTMIILLQVL IPISLYVSIE 370 380 390 400 410 420 LVKLGQVFFL SNDLDLYDEE TDLSIQCRAL NIAEDLGQIQ YIFSDKTGTL TENKMVFRRC 430 440 450 460 470 480 TIMGSEYSHQ ENGIEAPKGS IPLSKRKYPA LLRNEEIKDI LLALLEAVWH FHKLLPVSLW 490 500 SSLSQIRAVP ITCKLSFVYK G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Brain.
[2]	Ohara O., Suyama M., Nagase T., Ishikawa K., Kikuno R. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS B AND C). Tissue: Amygdala and Colon.
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB018258 mRNA. Translation: BAA34435.2. Different initiation. AK090832 mRNA. Translation: BAC03528.1. AK025130 mRNA. Translation: BAB15074.1. AC008456 Genomic DNA. No translation available.
RefSeq	NP_079429.2.
UniGene	Hs.109358
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000118322. Homo sapiens. [Contig view]
GeneID	23120.
KEGG	hsa:23120.
Organism-specific databases
HGNC	HGNC:13543. ATP10B.
PharmGKB	PA25098.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O94823.
HOVERGEN	O94823.
Gene expression databases
ArrayExpress	O94823.
CleanEx	HS_ATP10B.
GermOnline	ENSG00000118322. Homo sapiens.
Family and domain databases
InterPro	IPR001757. ATPase_P. IPR006539. Flippase. IPR013200. HAD-SF_hydro-like_3. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF08282. Hydrolase_3. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	O94823.
NextBio	44343.

Entry information

Entry name

AT10B_HUMAN

Accession

Primary (citable) accession number: O94823
Secondary accession number(s): Q9H725

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 30, 2003
Last modified:	November 25, 2008
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)