Reviewed, UniProtKB/Swiss-Prot O94829 (IPO13_HUMAN)

Names and origin

Protein names

Recommended name:
    Importin-13
      Short name=Imp13
Alternative name(s):
    Ran-binding protein 13
      Short name=RanBP13
    Karyopherin-13
      Short name=Kap13

Gene names

Name:	IPO13
Synonyms:	KIAA0724, RANBP13

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	963 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity. Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13.
Subunit structure	Interacts with UBC9, RBM8A, eIF-1A and PAX6.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	Expressed in fetal brain, heart, intestine and kidney.
Sequence similarities	Belongs to the importin beta family. Contains 14 HEAT repeats. Contains 1 importin N-terminal domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Gene Ontology (GO)
Biological process	protein import into nucleus, docking Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro nuclear pore Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ALX1	Q15699	1	EBI-747310,EBI-750671

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 963	963	Importin-13		PRO_0000120758
Regions
	Domain	45 – 111	67	Importin N-terminal
	Repeat	164 – 195	32	HEAT 1
	Repeat	196 – 235	40	HEAT 2
	Repeat	251 – 276	26	HEAT 3
	Repeat	277 – 314	38	HEAT 4
	Repeat	331 – 370	40	HEAT 5
	Repeat	388 – 437	50	HEAT 6
	Repeat	444 – 481	38	HEAT 7
	Repeat	482 – 522	41	HEAT 8
	Repeat	525 – 560	36	HEAT 9
	Repeat	599 – 634	36	HEAT 10
	Repeat	635 – 674	40	HEAT 11
	Repeat	679 – 717	39	HEAT 12
	Repeat	718 – 756	39	HEAT 13
	Repeat	788 – 830	43	HEAT 14
Experimental info
	Sequence conflict	202	1	F → V in CAB55966. Ref.6
	Sequence conflict	715	1	T → A in BAB14575. Ref.7
	Sequence conflict	817	1	A → S in AAH08194. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O94829-1 [UniParc]. Last modified July 19, 2004. Version 3. Checksum: 8C696EE2B92365F0	FASTA	963	108,195
	10 20 30 40 50 60 MERREEQPGA AGAGAAPALD FTVENVEKAL HQLYYDPNIE NKNLAQKWLM QAQVSPQAWH 70 80 90 100 110 120 FSWQLLQPDK VPEIQYFGAS ALHIKISRYW SDIPTDQYES LKAQLFTQIT RFASGSKIVL 130 140 150 160 170 180 TRLCVALASL ALSMMPDAWP CAVADMVRLF QAEDSPVDGQ GRCLALLELL TVLPEEFQTS 190 200 210 220 230 240 RLPQYRKGLV RTSLAVECGA VFPLLEQLLQ QPSSPSCVRQ KVLKCFSSWV QLEVPLQDCE 250 260 270 280 290 300 ALIQAAFAAL QDSELFDSSV EAIVNAISQP DAQRYVNTLL KLIPLVLGLQ EQLRQAVQNG 310 320 330 340 350 360 DMETSHGICR IAVALGENHS RALLDQVEHW QSFLALVNMI MFCTGIPGHY PVNETTSSLT 370 380 390 400 410 420 LTFWYTLQDD ILSFEAEKQA VYQQVYRPVY FQLVDVLLHK AQFPSDEEYG FWSSDEKEQF 430 440 450 460 470 480 RIYRVDISDT LMYVYEMLGA ELLSNLYDKL GRLLTSSEEP YSWQHTEALL YGFQSIAETI 490 500 510 520 530 540 DVNYSDVVPG LIGLIPRISI SNVQLADTVM FTIGALSEWL ADHPVMINSV LPLVLHALGN 550 560 570 580 590 600 PELSVSSVST LKKICRECKY DLPPYAANIV AVSQDVLMKQ IHKTSQCMWL MQALGFLLSA 610 620 630 640 650 660 LQVEEILKNL HSLISPYIQQ LEKLAEEIPN PSNKLAIVHI LGLLSNLFTT LDISHHEDDH 670 680 690 700 710 720 EGPELRKLPV PQGPNPVVVV LQQVFQLIQK VLSKWLNDAQ VVEAVCAIFE KSVKTLLDDF 730 740 750 760 770 780 APMVPQLCEM LGRMYSTIPQ ASALDLTRQL VHIFAHEPAH FPPIEALFLL VTSVTLTLFQ 790 800 810 820 830 840 QGPRDHPDIV DSFMQLLAQA LKRKPDLFLC ERLDVKAVFQ CAVLALKFPE APTVKASCGF 850 860 870 880 890 900 FTELLPRCGE VESVGKVVQE DGRMLLIAVL EAIGGQASRS LMDCFADILF ALNKHCFSLL 910 920 930 940 950 960 SMWIKEALQP PGFPSARLSP EQKDTFSQQI LRERVNKRRV KEMVKEFTLL CRGLHGTDYT ADY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Importin 13: a novel mediator of nuclear import and export." Mingot J.-M., Kostka S., Kraft R., Hartmann E., Goerlich D. EMBO J. 20:3685-3694(2001) [PubMed: 11447110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RBM8A; UBC9 AND EIF1A.
[2]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[6]	The German cDNA consortium Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-963. Tissue: Testis.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 495-963. Tissue: Placenta.
[8]	"Paired-type homeodomain transcription factors are imported into the nucleus by karyopherin 13." Ploski J.E., Shamsher M.K., Radu A. Mol. Cell. Biol. 24:4824-4834(2004) [PubMed: 15143176] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAX6.
[9]	"A novel karyopherin-beta homolog is developmentally and hormonally regulated in fetal lung." Zhang C., Sweezey N.B., Gagnon S., Muskat B., Koehler D., Post M., Kaplan F. Am. J. Respir. Cell Mol. Biol. 22:451-459(2000) [PubMed: 10745026] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF267987 mRNA. Translation: AAF73471.1. AB018267 mRNA. Translation: BAA34444.2. Different initiation. AL357079 Genomic DNA. Translation: CAI16798.1. CH471059 Genomic DNA. Translation: EAX07072.1. BC008194 mRNA. Translation: AAH08194.1. AL117501 mRNA. Translation: CAB55966.1. AK023441 mRNA. Translation: BAB14575.1. Different initiation.
PIR	T17276.
RefSeq	NP_055467.2.
UniGene	Hs.158497
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O94829.
PTM databases
PhosphoSite	O94829.
Genome annotation databases
Ensembl	ENSG00000117408. Homo sapiens. [Contig view]
GeneID	9670.
KEGG	hsa:9670.
Organism-specific databases
H-InvDB	HIX0000511.
HGNC	HGNC:16853. IPO13.
MIM	610411. gene.
PharmGKB	PA134981096.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O94829.
HOVERGEN	O94829.
Gene expression databases
ArrayExpress	O94829.
CleanEx	HS_IPO13.
GermOnline	ENSG00000117408. Homo sapiens.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR013598. Exportin-1/Importin-b-like. IPR000357. HEAT. IPR001494. Importin-b_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF03810. IBN_N. 1 hit. PF08389. Xpo1. 1 hit. [Graphical view]
PROSITE	PS50077. HEAT_REPEAT. False negative. PS50166. IMPORTIN_B_NT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	36311.
SOURCE	Search...

Entry information

Entry name

IPO13_HUMAN

Accession

Primary (citable) accession number: O94829
Secondary accession number(s): Q5T4X3 , Q7LC04, Q96HS3, Q9H8N3, Q9UFR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)