Reviewed, UniProtKB/Swiss-Prot O94989 (ARHGF_HUMAN)

Names and origin

Protein names

Recommended name:
    Rho guanine nucleotide exchange factor 15
Alternative name(s):
    Vsm-RhoGEF

Gene names

Name:	ARHGEF15
Synonyms:	KIAA0915

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specific GEF for RhoA activation and the regulation of vascular smooth muscle contractility.
Subunit structure	Interacts with EPHA4.
Tissue specificity	Expressed in the vascular smooth muscle of coronary artery.
Post-translational modification	Phosphorylated on tyrosine residues upon EFNA1 stimulation.
Sequence similarities	Contains 1 DH (DBL-homology) domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	GTPase activation Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O94989-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O94989-2)

The sequence of this isoform differs from the canonical sequence as follows:
     201-234: DAGLACPPCCPCVCHTTRPGLELRWVPVGGYEEV → GECGGWGALGDLNPPIFSVFSANNFQGLPFQKWS
     235-841: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 841	841	Rho guanine nucleotide exchange factor 15		PRO_0000080932
Regions
	Domain	417 – 601	185	DH
	Compositional bias	7 – 133	127	Pro-rich
Natural variations
	Alternative sequence	201 – 234	34	DAGLA…GYEEV → GECGGWGALGDLNPPIFSVF SANNFQGLPFQKWS in isoform 2.		VSP_011160
	Alternative sequence	235 – 841	607	Missing in isoform 2.		VSP_011161
Experimental info
	Sequence conflict	155	1	G → V in AAH36749. Ref.4
	Sequence conflict	277	1	L → P in AAH36749. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 16, 2004. Version 3. Checksum: 40D43363F777002F	FASTA	841	91,950
	10 20 30 40 50 60 MSAQSLPAAT PPTQKPPRII RPRPPSRSRA AQSPGPPHNG SSPQELPRNS NDAPTPMCTP 70 80 90 100 110 120 IFWEPPAASL KPPALLPPSA SRASLDSQTS PDSPSSTPTP SPVSRRSASP EPAPRSPVPP 130 140 150 160 170 180 PKPSGSPCTP LLPMAGVLAQ NGSASAPGTV RRLAGRFEGG AEGRAQDADA PEPGLQARAD 190 200 210 220 230 240 VNGEREAPLT GSGSQENGAP DAGLACPPCC PCVCHTTRPG LELRWVPVGG YEEVPRVPRR 250 260 270 280 290 300 ASPLRTSRSR PHPPSIGHPA VVLTSYRSTA ERKLLPLLKP PKPTRVRQDA TIFGDPPQPD 310 320 330 340 350 360 LDLLSEDGIQ TGDSPDEAPQ NTPPATVEGR EEEGLEVLKE QNWELPLQDE PLYQTYRAAV 370 380 390 400 410 420 LSEELWGVGE DGSPSPANAG DAPTFPRPPG PRNTLWQELP AVQASGLLDT LSPQERRMQE 430 440 450 460 470 480 SLFEVVTSEA SYLRSLRLLT DTFVLSQALR DTLTPRDHHT LFSNVQRVQG VSERFLATLL 490 500 510 520 530 540 SRVRSSPHIS DLCDVVHAHA VGPFSVYVDY VRNQQYQEET YSRLMDTNVR FSAELRRLQS 550 560 570 580 590 600 LPKCERLPLP SFLLLPFQRI TRLRMLLQNI LRQTEEGSSR QENAQKALGA VSKIIERCSA 610 620 630 640 650 660 EVGRMKQTEE LIRLTQRLRF HKVKALPLVS WSRRLEFQGE LTELGCRRGG VLFASRPRFT 670 680 690 700 710 720 PLCLLLFSDL LLITQPKSGQ RLQVLDYAHR SLVQAQQVPD PSGPPTFRLS LLSNHQGRPT 730 740 750 760 770 780 HRLLQASSLS DMQRWLGAFP TPGPLPCSPD TIYEDCDCSQ ELCSESSAPA KTEGRSLESR 790 800 810 820 830 840 AAPKHLHKTP EGWLKGLPGA FPAQLVCEVT GEHERRRHLR QNQRLLEAVG PSSGTPNAPP P
	Isoform 2 [UniParc]. Checksum: F9DADE62AA37E5CE		234	23,956
	10 20 30 40 50 60 MSAQSLPAAT PPTQKPPRII RPRPPSRSRA AQSPGPPHNG SSPQELPRNS NDAPTPMCTP 70 80 90 100 110 120 IFWEPPAASL KPPALLPPSA SRASLDSQTS PDSPSSTPTP SPVSRRSASP EPAPRSPVPP 130 140 150 160 170 180 PKPSGSPCTP LLPMAGVLAQ NGSASAPGTV RRLAGRFEGG AEGRAQDADA PEPGLQARAD 190 200 210 220 230 VNGEREAPLT GSGSQENGAP GECGGWGALG DLNPPIFSVF SANNFQGLPF QKWS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thyroid.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[5]	"EphA4-mediated Rho activation via Vsm-RhoGEF expressed specifically in vascular smooth muscle cells." Ogita H., Kunimoto S., Kamioka Y., Sawa H., Masuda M., Mochizuki N. Circ. Res. 93:23-31(2003) [PubMed: 12775584] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION, INTERACTION WITH EPHA4, REGULATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB020722 mRNA. Translation: BAA74938.2. Different initiation. AK023853 mRNA. Translation: BAB14703.1. BC036749 mRNA. Translation: AAH36749.1.
RefSeq	NP_776089.2.
UniGene	Hs.443109
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O94989.
Genome annotation databases
Ensembl	ENSG00000198844. Homo sapiens. [Contig view]
GeneID	22899.
KEGG	hsa:22899.
Organism-specific databases
H-InvDB	HIX0013523.
HGNC	HGNC:15590. ARHGEF15.
MIM	608504. gene.
PharmGKB	PA24970.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O94989.
HOVERGEN	O94989.
Gene expression databases
ArrayExpress	O94989.
CleanEx	HS_ARHGEF15.
GermOnline	ENSG00000198844. Homo sapiens.
Family and domain databases
InterPro	IPR000219. DH-domain. IPR001331. GDS_CDC24_CS. [Graphical view]
Gene3D	G3DSA:1.20.900.10. RhoGEF. 1 hit.
Pfam	PF00621. RhoGEF. 1 hit. [Graphical view]
SMART	SM00325. RhoGEF. 1 hit. [Graphical view]
PROSITE	PS00741. DH_1. False negative. PS50010. DH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	43527.
SOURCE	Search...

Entry information

Entry name

ARHGF_HUMAN

Accession

Primary (citable) accession number: O94989
Secondary accession number(s): Q8N449, Q9H8B4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)