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Reviewed, UniProtKB/Swiss-Prot O95154 (ARK73_HUMAN)

Last modified July 22, 2008. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Aflatoxin B1 aldehyde reductase member 3
    EC=1.-.-.-
Alternative name(s):
    AFB1-AR 2
Gene names
Name: AKR7A3
Synonyms: AFAR2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen By similarity.

Subunit structure

Probably act as a homodimer By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

Expressed in colon, kidney, pancreas, adenocarcinoma and endometrium.

Sequence similarities

Belongs to the aldo/keto reductase 2 family.

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Ontologies

Keywords

   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical term3D-structure

Gene Ontology (GO)

   Biological processcellular aldehyde metabolic process Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentcytosol Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionaldo-keto reductase activity Ref.1

Traceable author statement. Source: ProtInc

electron carrier activity Ref.1

Traceable author statement. Source: UniProtKB

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 331331Aflatoxin B1 aldehyde reductase member 3

Regions

Nucleotide binding198 – 20811NADP By similarity

Sites

Active site491Proton donor By similarity
Binding site1131Substrate By similarity
Site771Lowers pKa of active site Tyr By similarity

Natural variations

Natural variant1381V → M
Natural variant2151N → D: dbSNP rs1738023.
Natural variant3231T → A: dbSNP rs1738025.

Experimental info

Sequence conflict511E → D in AAD02195. Ref.1
Sequence conflict2471L → P in AAH42420. Ref.4

Secondary structure

1 .......................................................... 331
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
O95154-1 [UniParc].

Last modified February 21, 2001. Version 2.
Checksum: B9C32C33C7102AB3

FASTA33137,206
        10         20         30         40         50         60 
MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFVYS EGQSETILGG 

        70         80         90        100        110        120 
LGLRLGGSDC RVKIDTKAIP LFGNSLKPDS LRFQLETSLK RLQCPRVDLF YLHMPDHSTP 

       130        140        150        160        170        180 
VEETLRACHQ LHQEGKFVEL GLSNYAAWEV AEICTLCKSN GWILPTVYQG MYNAITRQVE 

       190        200        210        220        230        240 
TELFPCLRHF GLRFYAFNPL AGGLLTGKYK YEDKNGKQPV GRFFGNTWAE MYRNRYWKEH 

       250        260        270        280        290        300 
HFEGIALVEK ALQAAYGASA PSMTSATLRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA 

       310        320        330 
AAEEGPLEPA VVDAFNQAWH LVTHECPNYF R

« Hide

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References

« Hide 'large scale' references
[1]"cDNA cloning, expression and activity of a second human aflatoxin B1-metabolizing member of the aldo-keto reductase superfamily, AKR7A3."
Knight L.P., Primiano T., Groopman J.D., Kensler T.W., Sutter T.R.
Carcinogenesis 20:1215-1223(1999) [PubMed: 10383892] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, VARIANTS MET-138; ASP-215 AND ALA-323.
Tissue: Liver.
[2]"Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells."
Praml C., Savelyeva L., Schwab M.
Oncogene 22:4765-4773(2003) [PubMed: 12879023] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANTS ASP-215 AND ALA-323.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS MET-138; ASP-215 AND ALA-323.

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Cross-references

Sequence databases

AF040639 mRNA. Translation: AAD02195.1.
AJ271799 mRNA. Translation: CAC81076.1.
AL035413 Genomic DNA. Translation: CAI22232.1.
BC025709 mRNA. Translation: AAH25709.1.
BC031562 mRNA. Translation: AAH31562.1.
BC042420 mRNA. Translation: AAH42420.2.
RefSeqNP_036199.2.
UniGeneHs.6980

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2CLPX-ray3.00A/B/C/D/E/F/G/H/I/J/K6-331[»]
ModBaseSearch...

Genome annotation databases

EnsemblENSG00000162482. Homo sapiens. [Contig view]
GeneID22977.
KEGGhsa:22977.

Organism-specific databases

H-InvDBHIX0000196.
HGNCHGNC:390. AKR7A3.
MIM608477. gene.
PharmGKBPA24683.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMO95154.
HOVERGENO95154.

Gene expression databases

ArrayExpressO95154.
CleanExHS_AKR7A3.
GermOnlineENSG00000162482. Homo sapiens.

Family and domain databases

InterProIPR001395. Aldo/ket_red.
[Graphical view]
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSPR00069. ALDKETRDTASE.
ProDomPD000288. Aldo/ket_red. 1 hit.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

SOURCESearch...
ProtoNetSearch...

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Entry information

Entry nameARK73_HUMAN
AccessionPrimary (citable) accession number: O95154
Secondary accession number(s): Q86SR4 expand/collapse secondary AC list , Q8IVN6, Q8N5V6, Q8TAX1, Q9NUC3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: July 22, 2008
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents