Reviewed, UniProtKB/Swiss-Prot O95376 (ARI2_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein ariadne-2 homolog
      Short name=ARI-2
Alternative name(s):
    Triad1 protein

Gene names

Name:	ARIH2
Synonyms:	ARI2, TRIAD1
ORF Names:	HT005

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
Subunit structure	Interacts with UBE2L3 By similarity.
Subcellular location	Nucleus.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
Sequence caution	The sequence AAG09696.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	multicellular organismal development Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	zinc ion binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 493	493	Protein ariadne-2 homolog
Regions
	Zinc finger	139 – 192	54	RING-type 1
	Zinc finger	208 – 270	63	IBR-type
	Zinc finger	300 – 344	45	RING-type 2
	Coiled coil	369 – 400	32	Potential
	Coiled coil	439 – 492	54	Potential
	Compositional bias	4 – 75	72	Asp/Glu-rich (acidic)
	Compositional bias	22 – 29	8	Poly-Glu
Amino acid modifications
	Modified residue	353	1	Phosphoserine
Experimental info
	Sequence conflict	280 – 281	2	CA → LQ in CAA10276. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O95376-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 30AFFDD327B51013	FASTA	493	57,819
	10 20 30 40 50 60 MSVDMNSQGS DSNEEDYDPN CEEEEEEEED DPGDIEDYYV GVASDVEQQG ADAFDPEEYQ 70 80 90 100 110 120 FTCLTYKESE GALNEHMTSL ASVLKVSHSV AKLILVNFHW QVSEILDRYK SNSAQLLVEA 130 140 150 160 170 180 RVQPNPSKHV PTSHPPHHCA VCMQFVRKEN LLSLACQHQF CRSCWEQHCS VLVKDGVGVG 190 200 210 220 230 240 VSCMAQDCPL RTPEDFVFPL LPNEELREKY RRYLFRDYVE SHYQLQLCPG ADCPMVIRVQ 250 260 270 280 290 300 EPRARRVQCN RCNEVFCFKC RQMYHAPTDC ATIRKWLTKC ADDSETANYI SAHTKDCPKC 310 320 330 340 350 360 NICIEKNGGC NHMQCSKCKH DFCWMCLGDW KTHGSEYYEC SRYKENPDIV NQSQQAQARE 370 380 390 400 410 420 ALKKYLFYFE RWENHNKSLQ LEAQTYQRIH EKIQERVMNN LGTWIDWQYL QNAAKLLAKC 430 440 450 460 470 480 RYTLQYTYPY AYYMESGPRK KLFEYQQAQL EAEIENLSWK VERADSYDRG DLENQMHIAE 490 QRRRTLLKDF HDT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TRIADs: a new class of proteins with a novel cysteine-rich signature." van der Reijden B.A., Erpelinck-Verschueren C.A.J., Loewenberg B., Jansen J.H. Protein Sci. 8:1557-1561(1999) [PubMed: 10422847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins." Aguilera M., Oliveros M., Martinez-Padron M., Barbas J.A., Ferrus A. Genetics 155:1231-1244(2000) [PubMed: 10880484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hypothalamus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF099149 mRNA. Translation: AAC82469.1. AJ130978 mRNA. Translation: CAA10276.1. AF183427 mRNA. Translation: AAG09696.1. Frameshift. BC000422 mRNA. Translation: AAH00422.1.
RefSeq	NP_006312.1.
UniGene	Hs.649132
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O95376.
PTM databases
PhosphoSite	O95376.
Genome annotation databases
Ensembl	ENSG00000177479. Homo sapiens. [Contig view]
GeneID	10425.
KEGG	hsa:10425.
Organism-specific databases
H-InvDB	HIX0021402.
HGNC	HGNC:690. ARIH2.
MIM	605615. gene.
PharmGKB	PA24983.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O95376.
HOVERGEN	O95376.
Gene expression databases
ArrayExpress	O95376.
CleanEx	HS_ARIH2.
GermOnline	ENSG00000177479. Homo sapiens.
Family and domain databases
InterPro	IPR002867. Znf_C6HC. IPR001878. Znf_CCHC. IPR001841. Znf_RING. [Graphical view]
Pfam	PF01485. IBR. 1 hit. [Graphical view]
SMART	SM00647. IBR. 2 hits. SM00184. RING. 2 hits. SM00343. ZnF_C2HC. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 2 hits. [Graphical view]
ProDom	O95376. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	O95376.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ARI2_HUMAN

Accession

Primary (citable) accession number: O95376
Secondary accession number(s): Q9HBZ6, Q9UEM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 1999
Last modified:	July 22, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)