Reviewed, UniProtKB/Swiss-Prot O95848 (NUD14_HUMAN)

Names and origin

Protein names

Recommended name:
    Uridine diphosphate glucose pyrophosphatase
      Short name=UDPG pyrophosphatase
      Short name=UGPPase
    EC=3.6.1.45
Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 14
      Short name=Nudix motif 14

Gene names

Name:	NUDT14
Synonyms:	UGPP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP-mannose.
Catalytic activity	UDP-sugar + H(2)O = UMP + alpha-D-aldose 1-phosphate.
Cofactor	Magnesium.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the Nudix hydrolase family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.0-9.5.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-sugar diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARAF	P10398	3	EBI-536866,EBI-365961

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Uridine diphosphate glucose pyrophosphatase		PRO_0000057113
Regions
	Motif	111 – 129	19	Nudix box

Sequences

	Sequence		Length	Mass (Da)
	O95848-1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: FB1DFA40A67E72B6	FASTA	222	24,118
	10 20 30 40 50 60 MERIEGASVG RCAASPYLRP LTLHYRQNGA QKSWDFMKTH DSVTVLLFNS SRRSLVLVKQ 70 80 90 100 110 120 FRPAVYAGEV ERRFPGSLAA VDQDGPRELQ PALPGSAGVT VELCAGLVDQ PGLSLEEVAC 130 140 150 160 170 180 KEAWEECGYH LAPSDLRRVA TYWSGVGLTG SRQTMFYTEV TDAQRSGPGG GLVEEGELIE 190 200 210 220 VVHLPLEGAQ AFADDPDIPK TLGVIFGVSW FLSQVAPNLD LQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization, chromosomal localization, and the complete 30-kb DNA sequence of the human Jagged2 (JAG2) gene." Deng Y., Madan A., Banta A.B., Friedman C., Trask B.J., Hood L., Li L. Genomics 63:133-138(2000) [PubMed: 10662552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning, expression and characterization of a mammalian Nudix hydrolase-like enzyme that cleaves the pyrophosphate bond of UDP-glucose." Yagi T., Baroja-Fernandez E., Yamamoto R., Munoz F.J., Akazawa T., Hong K.S., Pozueta-Romero J. Biochem. J. 370:409-415(2003) [PubMed: 12429023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, SUBCELLULAR LOCATION, SUBUNIT. Tissue: Thyroid.
[3]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF111170 Genomic DNA. Translation: AAD15563.1. Different initiation. AB087802 mRNA. Translation: BAC65455.1. AL512355 Genomic DNA. No translation available. BC041584 mRNA. Translation: AAH41584.1.
RefSeq	NP_803877.2.
UniGene	Hs.526432
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O95848.
Genome annotation databases
Ensembl	ENSG00000183828. Homo sapiens. [Contig view]
GeneID	256281.
KEGG	hsa:256281.
NMPDR	fig|9606.3.peg.10277.
Organism-specific databases
H-InvDB	HIX0026628.
HGNC	HGNC:20141. NUDT14.
MIM	609219. gene.
PharmGKB	PA134971372.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O95848.
HOVERGEN	O95848.
Gene expression databases
ArrayExpress	O95848.
CleanEx	HS_NUDT14.
GermOnline	ENSG00000183828. Homo sapiens.
Family and domain databases
InterPro	IPR004385. NDP_pyrophos. IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
TIGRFAMs	TIGR00052. NDP_pyrophos. 1 hit.
PROSITE	PS00893. NUDIX. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	92764.
SOURCE	Search...

Entry information

Entry name

NUD14_HUMAN

Accession

Primary (citable) accession number: O95848
Secondary accession number(s): Q86SJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	November 25, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)