Reviewed, UniProtKB/Swiss-Prot O96009 (NAPSA_HUMAN)

Names and origin

Protein names

Recommended name:
    Napsin-A
    EC=3.4.23.-
Alternative name(s):
    Napsin-1
    NAPA
    TA01/TA02
    Aspartyl protease 4
      Short name=Asp 4
      Short name=ASP4

Gene names

Name:	NAPSA
Synonyms:	NAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in processing of pneumocyte surfactant precursors.
Tissue specificity	Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Ref.1 Traceable author statement. Source: ProtInc
Molecular function	aspartic-type endopeptidase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 63	38	Activation peptide
	Chain	64 – 420	357	Napsin-A
Sites
	Active site	96	1	By similarity
	Active site	283	1	By similarity
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 116		By similarity
	Disulfide bond	274 ↔ 278		By similarity
	Disulfide bond	317 ↔ 354		By similarity
Natural variations
	Natural variant	310	1	A → T: dbSNP rs11670727.

Sequences

	Sequence		Length	Mass (Da)
	O96009-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 018B86AE5BD0C865	FASTA	420	45,387
	10 20 30 40 50 60 MSPPPLLQPL LLLLPLLNVE PSGATLIRIP LHRVQPGRRI LNLLRGWREP AELPKLGAPS 70 80 90 100 110 120 PGDKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS NLWVPSRRCH FFSVPCWLHH 130 140 150 160 170 180 RFDPKASSSF QANGTKFAIQ YGTGRVDGIL SEDKLTIGGI KGASVIFGEA LWEPSLVFAF 190 200 210 220 230 240 AHFDGILGLG FPILSVEGVR PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP 250 260 270 280 290 300 AHYIPPLTFV PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH 310 320 330 340 350 360 AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG VRLCLSGFQA 370 380 390 400 410 420 LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR ARTRGADLGW GETAQAQFPG

References

[1]	"Napsins: new human aspartic proteinases. Distinction between two closely related genes." Tatnell P.J., Powell D.J., Hill J., Smith T.S., Tew D.G., Kay J. FEBS Lett. 441:43-48(1998) [PubMed: 9877162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney and Lung.
[2]	"New human aspartic proteases napsin 1 and napsin 2: molecular cloning and intracellular localization of napsin 1." Koelsch G., Wu S., Henthorn J., Tang J., Lin X. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas." Chuman Y., Bergman A.-C., Ueno T., Saito S., Sakaguchi K., Alaiya A.A., Franzen B., Bergman T., Arnott D., Auer G., Appella E., Joernvall H., Linder S. FEBS Lett. 462:129-134(1999) [PubMed: 10580105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Fetal lung.
[4]	Bienkowski M.J., Shuck M.E., Slightom J.L., Drong R.F. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF090386 mRNA. Translation: AAD04917.1. AF098484 mRNA. Translation: AAD13215.1. AF200345 mRNA. Translation: AAF17081.1.
RefSeq	NP_004842.1.
UniGene	Hs.512843
3D structure databases
HSSP	HSSP built from PDB template 2REN based on UniProtKB P00797.
ModBase	Search...
Protein family/group databases
MEROPS	A01.046.
Genome annotation databases
Ensembl	ENSG00000131400. Homo sapiens. [Contig view]
GeneID	9476.
KEGG	hsa:9476.
Organism-specific databases
H-InvDB	HIX0015357. HIX0039966.
HGNC	HGNC:13395. NAPSA.
HPA	CAB009591.
MIM	605631. gene.
PharmGKB	PA134891814.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O96009.
HOVERGEN	O96009.
Gene expression databases
ArrayExpress	O96009.
CleanEx	HS_NAPSA.
GermOnline	ENSG00000131400. Homo sapiens.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

NAPSA_HUMAN

Accession

Primary (citable) accession number: O96009

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	July 22, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)