Reviewed, UniProtKB/Swiss-Prot O96621 (ARP2_DICDI)

Names and origin

Protein names

Recommended name:
    Actin-related protein 2
Alternative name(s):
    Actin-like protein 2
    Actin-related protein B

Gene names

Name:	arpB
Synonyms:	arp2
ORF Names:	DDB_G0272106

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. The Arp2/3 complex is involved in organizing the actin system in cell motility and chemotaxis, in phagocytosis and macropinocytosis, at late steps of endosome processing, and in mitosis. In concert with a group of other proteins, the Arp2/3 complex plays a general role in the rapid activation and adaptation of the actin system to its multiple functions.
Subunit structure	Component of the Arp2/3 complex composed of arpB/Arp2, arpC/Arp3, arcA/p41-arc, arcB/p34-arc, arcC/p21-arc, arcD/p20-arc and arcE/p16-arc.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection. Cytoplasm › cytosol. Cytoplasm › cell cortex.
Sequence similarities	Belongs to the actin family. ARP2 subfamily.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Ligand	ATP-binding Actin-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin filament polymerization Ref.4 Inferred from mutant phenotype. Source: dictyBase actin nucleation Traceable author statement. Source: dictyBase bleb formation Inferred from mutant phenotype. Source: dictyBase cell motion Traceable author statement. Source: dictyBase hyperosmotic response Inferred from expression pattern. Source: dictyBase
Cellular component	Arp2/3 protein complex Ref.5 Inferred from direct assay. Source: dictyBase cell leading edge Traceable author statement. Source: dictyBase cortical actin cytoskeleton Inferred by curator. Source: dictyBase filopodium Inferred by curator. Source: dictyBase phagocytic vesicle Traceable author statement. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Actin-related protein 2		PRO_0000327509
Regions
	Nucleotide binding	158 – 160	3	ATP By similarity
	Nucleotide binding	212 – 216	5	ATP By similarity
	Nucleotide binding	303 – 308	6	ATP By similarity
	Compositional bias	194 – 197	4	Poly-Leu

Sequences

	Sequence		Length	Mass (Da)
	O96621-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 1BC8D7C0C3222304	FASTA	392	44,238
	10 20 30 40 50 60 MDSNKVIVMD NGTGFVKCGF AGANFPTAIF PSMVGRPILR SEEKVENVEI KDIMVGDEAA 70 80 90 100 110 120 KLRSMLQITY PLENGIIRNW DDITHVWDYA LKEKLKVSDP TECKILLTEP PMNPVANRQK 130 140 150 160 170 180 MIECMFEKYG FQAVYVAIQA VLTLYAQGLL TGVVVDSGDG VTHIIPVYEG YSIPHLTRRL 190 200 210 220 230 240 DVAGRDVTRY LIKLLLLRGY AFNRTADFET IRQIKEKLCY VAYDVQQEMK LASETTVLVE 250 260 270 280 290 300 NYTLPDGRVI KVGQERFQAS EALFNPSLVD VEGGGVHEQL FDCITKADRD LQQGFYQHIV 310 320 330 340 350 360 LSGGSSMYPG LPSRLEKEIR SLYLERVLKG NKEGLAKFKC RIEDPPRRKH MVFLGGAVLA 370 380 390 DLTKDRDDFW ITKAEYMEKG FGALDKLTKL SV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis." Insall R., Mueller-Taubenberger A., Machesky L., Koehler J., Simmeth E., Atkinson S.J., Weber I., Gerisch G. Cell Motil. Cytoskeleton 50:115-128(2001) [PubMed: 11807934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Mutants in the Dictyostelium Arp2/3 complex and chemoattractant-induced actin polymerization." Langridge P.D., Kay R.R. Exp. Cell Res. 313:2563-2574(2007) [PubMed: 17553489] [Abstract] Cited for: FUNCTION, MUTAGENESIS.
[5]	"Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium." Meima M.E., Weening K.E., Schaap P. Protein Expr. Purif. 53:283-288(2007) [PubMed: 17296313] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF095929 mRNA. Translation: AAC99776.1. AC116963 Genomic DNA. Translation: AAS45353.1. AAFI02000008 Genomic DNA. Translation: EAL71205.1.
RefSeq	XP_645275.1.
3D structure databases
HSSP	HSSP built from PDB template 1K8K based on UniProtKB P61157.
ModBase	Search...
Genome annotation databases
GeneID	3396277.
KEGG	ddi:DDB_0185179.
Organism-specific databases
dictyBase	DDB0185179. arpB.
Family and domain databases
InterPro	IPR004000. Actin_like. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARP2_DICDI

Accession

Primary (citable) accession number: O96621
Secondary accession number(s): Q559Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)