Reviewed, UniProtKB/Swiss-Prot O96622 (ARPC1_DICDI)

Names and origin

Protein names

Recommended name:
    Actin-related protein 2/3 complex subunit 1
Alternative name(s):
    Arp2/3 complex 41 kDa subunit
    p41-Arc

Gene names

Name:	arcA
Synonyms:	Arc40, arpD
ORF Names:	DDB_0214932

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. The Arp2/3 complex is involved in organizing the actin system in cell motility and chemotaxis, in phagocytosis and macropinocytosis, at late steps of endosome processing, and in mitosis. In concert with a group of other proteins, the Arp2/3 complex plays a general role in the rapid activation and adaptation of the actin system to its multiple functions.
Subunit structure	Component of the Arp2/3 complex composed of arpB/Arp2, arpC/Arp3, arcA/p41-arc, arcB/p34-arc, arcC/p21-arc, arcD/p20-arc and arcE/p16-arc.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection. Cytoplasm › cytosol. Cytoplasm › cell cortex.
Sequence similarities	Belongs to the WD repeat ARPC1 family. Contains 5 WD repeats.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Domain	Repeat WD repeat
Ligand	Actin-binding
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 369	369	Actin-related protein 2/3 complex subunit 1
Regions
	Repeat	8 – 47	40	WD 1
	Repeat	52 – 91	40	WD 2
	Repeat	141 – 180	40	WD 3
	Repeat	200 – 239	40	WD 4
	Repeat	322 – 365	44	WD 5

Sequences

	Sequence		Length	Mass (Da)
	O96622-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E967BDCDFD236811	FASTA	369	41,066
	10 20 30 40 50 60 MSAFEIEHLA SCITAHAWNA DRSRVALCPN NNEVHIYAKQ GTSWVVEHVL AEHDQLVTSI 70 80 90 100 110 120 DWAPKTNRIL TSSQDRNAYV WTFKDGQWKP VLVLLRINRA ATHVKWSPQE NKFAVATGAK 130 140 150 160 170 180 LVCICFFEEE HDWWASNHIK KHKSTVLKVD WHPNNLLLAT SSSDYKVRVF DAYIKKADGR 190 200 210 220 230 240 SVTRPYGEVA FGEPVFEFDQ CASWVHALKW SPSGSTLAYS SHDGVFAVAN FSTNPPTIEK 250 260 270 280 290 300 LRVRNLPLRD LLYITENSIA GVGYDCAPLL ITNQNGWKYS GEMDKASEGG AAAGSETSAR 310 320 330 340 350 360 KLFQNKVDLG ESKSADKKLT TVHQNCITSI VPFKSVGGVV SDFSTSGLDG NIVVWHVKAL EAKNKFKVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis." Insall R., Mueller-Taubenberger A., Machesky L., Koehler J., Simmeth E., Atkinson S.J., Weber I., Gerisch G. Cell Motil. Cytoskeleton 50:115-128(2001) [PubMed: 11807934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium." Meima M.E., Weening K.E., Schaap P. Protein Expr. Purif. 53:283-288(2007) [PubMed: 17296313] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF095930 mRNA. Translation: AAC99777.1. AAFI02000023 Genomic DNA. Translation: EAL68085.1.
RefSeq	XP_642137.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3393159.
KEGG	ddi:DDB_0214932.
Organism-specific databases
dictyBase	DDB0214932. arcA.
Family and domain databases
InterPro	IPR017383. ARPC2/3_su1. IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 3 hits. [Graphical view]
PIRSF	PIRSF038093. ARP2/3_su1. 1 hit.
PRINTS	PR00320. GPROTEINBRPT.
ProDom	PD000018. WD40. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00320. WD40. 4 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ARPC1_DICDI

Accession

Primary (citable) accession number: O96622
Secondary accession number(s): Q54YR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 1, 1999
Last modified:	September 23, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)