Reviewed, UniProtKB/Swiss-Prot O97401 (GUN_PHACE)

Names and origin

Protein names	Recommended name:     Endoglucanase     EC=3.2.1.4 Alternative name(s):     Endo-1,4-beta-glucanase     Cellulase
Organism	Phaedon cochleariae (Mustard beetle)
Taxonomic identifier	80249 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Chrysomelini › Phaedon

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	SecretedPotential.
Tissue specificity	Expressed in larval carcasses and gut, and adult gut.
Developmental stage	Eggs, larvae and adult.
Sequence similarities	Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Digestion Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW digestion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 242	221	Endoglucanase		PRO_5000147329
Sites
	Active site	47	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	217	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O97401-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: E57A9EC9DAE4379D	FASTA	242	25,336
	10 20 30 40 50 60 MQVIVLPLVF LATFATSGSL AAPDASPEIV PVDGGLSGYG TTTRYWDCCK PSCAWKENIN 70 80 90 100 110 120 TPTMTPVQTC AIDGNTVVNA SVQSGCIGGS SYMCSNQQAF VVNSTLAFGF AAGSFTGGVD 130 140 150 160 170 180 NNLCCSCMLL TFQGQLAGKQ FLVQITNTGG DLGSTSSIWP FPGGGVGIFT QGCHDQWTPR 190 200 210 220 230 240 GAAGGDQYGG VYSVEQCSDL PEVLQPGCRF RFEFLENVSN PQVSFQQVQC PAEIVAISNC AL

References

[1]

"Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae." Girard C., Jouanin L. Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed: 10612046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Larval gut.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17907 mRNA. Translation: CAA76931.1.
3D structure databases
HSSP	HSSP built from PDB template 2ENG based on UniProtKB P43316.
ModBase	Search...
Family and domain databases
InterPro	IPR014733. Barwin-like. IPR000334. Glyco_hydro_45. [Graphical view]
Gene3D	G3DSA:2.40.40.10. Barwin. 1 hit.
Pfam	PF02015. Glyco_hydro_45. 1 hit. [Graphical view]
PROSITE	PS01140. GLYCOSYL_HYDROL_F45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_PHACE

Accession

Primary (citable) accession number: O97401
Secondary accession number(s): P81522

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)