Reviewed, UniProtKB/Swiss-Prot P00045 (CYC7_YEAST)

Names and origin

Protein names

Recommended name:
    Cytochrome c iso-2

Gene names

Name:	CYC7
Synonyms:	CYP3
Ordered Locus Names:	YEL039C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular location	Mitochondrion intermembrane space.
Developmental stage	Iso-2 is the predominant cytochrome c in anaerobically grown or heme deficient cells.
Induction	By oxygen and low cAMP levels.
Post-translational modification	Binds 1 heme group per subunit.
Miscellaneous	Present with 1310 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cytochrome c family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay. Source: SGD mitochondrial electron transport, ubiquinol to cytochrome c Inferred from direct assay. Source: SGD transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial intermembrane space Inferred from direct assay. Source: SGD mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 113

113

Cytochrome c iso-2

PRO_0000108338

Sites

Metal binding

28

1

Iron (heme axial ligand)

Metal binding

90

1

Iron (heme axial ligand)

Binding site

24

1

Heme (covalent)

Binding site

27

1

Heme (covalent)

Secondary structure

1

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113

Helix Strand Turn

Details...

Helix

3 – 6

4

Helix

13 – 23

11

Turn

24 – 27

4

Helix

45 – 47

3

Beta strand

48 – 51

4

Helix

60 – 65

6

Helix

71 – 79

9

Helix

81 – 84

4

Helix

98 – 111

14

Sequences

	Sequence		Length	Mass (Da)
	P00045-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 50CBD0F95B480CB7	FASTA	113	12,532
	10 20 30 40 50 60 MAKESTGFKP GSAKKGATLF KTRCQQCHTI EEGGPNKVGP NLHGIFGRHS GQVKGYSYTD 70 80 90 100 110 ANINKNVKWD EDSMSEYLTN PKKYIPGTKM AFAGLKKEKD RNDLITYMTK AAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae." Montgomery D.L., Leung D.W., Smith M., Shalit P., Faye G., Hall B.D. Proc. Natl. Acad. Sci. U.S.A. 77:541-545(1980) [PubMed: 6244566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The gene clusters ARC and COR on chromosomes 5 and 10, respectively, of Saccharomyces cerevisiae share a common ancestry." Melnick L., Sherman F. J. Mol. Biol. 233:372-388(1993) [PubMed: 8411151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B-6441.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Structure determination and analysis of yeast iso-2-cytochrome c and a composite mutant protein." Murphy M.E.P., Nall B.T., Brayer G.D. J. Mol. Biol. 227:160-176(1992) [PubMed: 1326054] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Cross-references

Sequence databases

EMBLGenBankDDBJ

S65964 Genomic DNA. Translation: AAD13974.1.
V01299 Genomic DNA. Translation: CAA24606.1.
L22173 Genomic DNA. Translation: AAA34940.1.
J01320 Genomic DNA. Translation: AAB59339.1.
U18779 Genomic DNA. Translation: AAB65003.1.
AY693032 Genomic DNA. Translation: AAT93051.1.

PIR

CCBYBC. A00038.

RefSeq

NP_010875.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YEA	X-ray	1.90	A	2-113	[»]
1YEB	X-ray	1.95	A	6-107	[»]
1YTC	X-ray	1.80	A	2-113	[»]
3CXH	X-ray	2.50	W	2-113	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:7178N.

Proteomic databases

PeptideAtlas

P00045.

Genome annotation databases

Ensembl

YEL039C. Saccharomyces cerevisiae. [Contig view]

GeneID

856672.

GenomeReviews

Gene locus YEL039C in contig U00092_GR.

KEGG

sce:YEL039C.

NMPDR

fig|4932.3.peg.1926.

Organism-specific databases

CYGD

YEL039c.

SGD

S000000765. CYC7.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P00045.

Gene expression databases

GermOnline

YEL039C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR009056. Cyt_c_monohaem.
IPR003088. Cyt_CI.
IPR002327. Cyt_CIAB.
[Graphical view]

Gene3D

G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.

PANTHER

PTHR11961. Cyt_CIAB. 1 hit.

Pfam

PF00034. Cytochrom_C. 1 hit.
[Graphical view]

PRINTS

PR00604. CYTCHRMECIAB.

ProDom

PD000375. Cyt_CIAB. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS51007. CYTC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P00045.

NextBio

982687.

Entry information

Entry name

CYC7_YEAST

Accession

Primary (citable) accession number: P00045

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 25, 2008
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)