Reviewed, UniProtKB/Swiss-Prot P00246 (FER_SPIPL)

Names and origin

Protein names	Recommended name:     Ferredoxin
Organism	Spirulina platensis
Taxonomic identifier	118562 [NCBI]
Taxonomic lineage	Bacteria › Cyanobacteria › Oscillatoriales › Arthrospira

Protein attributes

Sequence length	99 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Cofactor	Binds 1 2Fe-2S cluster.
Sequence similarities	Belongs to the 2Fe2S plant-type ferredoxin family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 99

98

Ferredoxin

PRO_0000189371

Regions

Domain

4 – 96

93

2Fe-2S ferredoxin-type

Sites

Metal binding

42

1

Iron-sulfur (2Fe-2S)

Metal binding

47

1

Iron-sulfur (2Fe-2S)

Metal binding

50

1

Iron-sulfur (2Fe-2S)

Metal binding

80

1

Iron-sulfur (2Fe-2S)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

99

Helix Strand Turn

Details...

Beta strand

5 – 9

5

Beta strand

16 – 20

5

Helix

27 – 33

7

Beta strand

41 – 46

6

Beta strand

48 – 60

13

Helix

69 – 73

5

Helix

79 – 81

3

Beta strand

83 – 93

11

Turn

95 – 97

3

Sequences

	Sequence		Length	Mass (Da)
	P00246-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 71F9CA733DB03741	FASTA	99	10,634
	10 20 30 40 50 60 MATYKVTLIN EAEGINETID CDDDTYILDA AEEAGLDLPY SCRAGACSTC AGTITSGTID 70 80 90 QSDQSFLDDD QIEAGYVLTC VAYPTSDCTI KTHQEEGLY

References

[1]	"The complete amino acid sequence of the Spirulina platensis ferredoxin." Tanaka M., Haniu M., Yasunobu K.T., Rao K.K., Hall D.O. Biochem. Biophys. Res. Commun. 69:759-765(1976) [PubMed: 817723] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-99.
[2]	"X-ray analysis of a [2Fe-2S] ferrodoxin from Spirulina platensis. Main chain fold and location of side chains at 2.5-A resolution." Tsukihara T., Fukuyama K., Nakamura M., Katsube Y., Tanaka N., Kakudo M., Wada K., Hase T., Matsubara H. J. Biochem. 90:1763-1773(1981) [PubMed: 6801028] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[3]	"Structure of S. platensis [2Fe-2S] ferredoxin and evolution of chloroplast-type ferrodoxins." Fukuyama K., Hase T., Matsumoto S., Tsukihara T., Katsube Y., Tanaka N., Kakudo M., Wada K., Hase T., Matsubara H. Nature 286:522-524(1980) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

FESGAL. A00250.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4FXC	X-ray	2.50	A	1-99	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR006058. 2Fe2S_fd_BS.
IPR012675. b-grasp_ferredoxin-like.
IPR010241. Fdx_pln.
IPR001041. Ferredoxin.
[Graphical view]

Gene3D

G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.

Pfam

PF00111. Fer2. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02008. fdx_plant. 1 hit.

PROSITE

PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P00246.

Entry information

Entry name

FER_SPIPL

Accession

Primary (citable) accession number: P00246

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)