Reviewed, UniProtKB/Swiss-Prot P00447 (SODM_YEAST)

Names and origin

Protein names

Recommended name:
    Superoxide dismutase [Mn], mitochondrial
    EC=1.15.1.1

Gene names

Name:	SOD2
Ordered Locus Names:	YHR008C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Catalytic activity	2 superoxide + 2 H(+) = O(2) + H(2)O(2).
Cofactor	Binds 1 manganese ion per subunit By similarity.
Subunit structure	Homotetramer.
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 10900 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the iron/manganese superoxide dismutase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	age-dependent response to reactive oxygen species during chronological cell aging Inferred from mutant phenotype. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW replicative cell aging Inferred from mutant phenotype. Source: SGD superoxide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from direct assay. Source: SGD
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW superoxide dismutase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion
	Chain	27 – 233	207	Superoxide dismutase [Mn], mitochondrial		PRO_0000032888
Sites
	Metal binding	52	1	Manganese By similarity
	Metal binding	107	1	Manganese By similarity
	Metal binding	194	1	Manganese By similarity
	Metal binding	198	1	Manganese By similarity
Amino acid modifications
	Modified residue	147	1	Phosphothreonine
	Modified residue	149	1	Phosphothreonine

Sequences

	Sequence		Length	Mass (Da)
	P00447-1 [UniParc]. Last modified October 23, 1986. Version 1. Checksum: 88A9391FBB31D06E	FASTA	233	25,774
	10 20 30 40 50 60 MFAKTAAANL TKKGGLSLLS TTARRTKVTL PDLKWDFGAL EPYISGQINE LHYTKHHQTY 70 80 90 100 110 120 VNGFNTAVDQ FQELSDLLAK EPSPANARKM IAIQQNIKFH GGGFTNHCLF WENLAPESQG 130 140 150 160 170 180 GGEPPTGALA KAIDEQFGSL DELIKLTNTK LAGVQGSGWA FIVKNLSNGG KLDVVQTYNQ 190 200 210 220 230 DTVTGPLVPL VAIDAWEHAY YLQYQNKKAD YFKAIWNVVN WKEASRRFDA GKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein." Marres C.A.M., van Loon A.P.G.M., Oudshoorn P., van Steeg H., Grivell L.A., Slater E.C. Eur. J. Biochem. 147:153-161(1985) [PubMed: 3882422] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Functional expression of the yeast Mn-superoxide dismutase gene in Escherichia coli requires deletion of the signal peptide sequence." Schrank I.S., Sims P.F., Oliver S.G. Gene 73:121-130(1988) [PubMed: 3072251] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
[5]	"The complete amino acid sequence of manganese-superoxide dismutase from Saccharomyces cerevisae." Ditlow C., Johansen J.T., Martin B.M., Svendsen I. Carlsberg Res. Commun. 47:81-91(1982) Cited for: PROTEIN SEQUENCE OF 27-233.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-147 AND THR-149, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02156 Genomic DNA. Translation: CAA26092.1. U10400 Genomic DNA. Translation: AAB68939.1. M24079 Genomic DNA. Translation: AAA35065.1. AY557821 Genomic DNA. Translation: AAS56147.1.
PIR	DSBYN. A00521.
RefSeq	NP_011872.1.
3D structure databases
HSSP	HSSP built from PDB template 1KKC based on UniProtKB Q92450.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4905N.
IntAct	P00447.
Proteomic databases
PeptideAtlas	P00447.
Genome annotation databases
Ensembl	YHR008C. Saccharomyces cerevisiae. [Contig view]
GeneID	856399.
GenomeReviews	Gene locus YHR008C in contig U00093_GR.
KEGG	sce:YHR008C.
NMPDR	fig|4932.3.peg.3017.
Organism-specific databases
CYGD	YHR008c.
SGD	S000001050. SOD2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P00447.
Gene expression databases
GermOnline	YHR008C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001189. SODismutase. [Graphical view]
PANTHER	PTHR11404. SODismutase. 1 hit.
Pfam	PF02777. Sod_Fe_C. 1 hit. PF00081. Sod_Fe_N. 1 hit. [Graphical view]
PIRSF	PIRSF000349. SODismutase. 1 hit.
PRINTS	PR01703. MNSODISMTASE.
ProDom	PD000475. SODismutase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00088. SOD_MN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P00447.
NextBio	981928.

Entry information

Entry name

SODM_YEAST

Accession

Primary (citable) accession number: P00447

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 23, 1986
Last modified:	November 25, 2008
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)