Reviewed, UniProtKB/Swiss-Prot P00818 (ACYP2_HORSE)

Names and origin

Protein names

Recommended name:
    Acylphosphatase-2
    EC=3.6.1.7
Alternative name(s):
    Acylphosphate phosphohydrolase 2
    Acylphosphatase, muscle type isozyme

Gene names

Name:	ACYP2
Synonyms:	ACYP

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	99 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Its physiological role is not yet clear.
Catalytic activity	An acylphosphate + H(2)O = a carboxylate + phosphate.
Sequence similarities	Belongs to the acylphosphatase family. Contains 1 acylphosphatase-like domain.

Ontologies

Keywords
Molecular function	Hydrolase
PTM	Acetylation Glutathionylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	acylphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 99

98

Acylphosphatase-2

PRO_0000158541

Regions

Domain

9 – 99

91

Acylphosphatase-like

Sites

Active site

24

1

Potential

Active site

42

1

Potential

Amino acid modifications

Modified residue

2

1

N-acetylserine

Modified residue

22

1

S-glutathionyl cysteine

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

99

Helix Strand Turn

Details...

Beta strand

7 – 15

9

Helix

26 – 34

9

Beta strand

37 – 41

5

Beta strand

47 – 55

9

Helix

56 – 64

9

Beta strand

65 – 68

4

Beta strand

72 – 74

3

Beta strand

79 – 89

11

Beta strand

92 – 98

7

Sequences

	Sequence		Length	Mass (Da)
	P00818-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 911DAF1820A53790	FASTA	99	11,148
	10 20 30 40 50 60 MSTARPLKSV DYEVFGRVQG VCFRMYAEDE ARKIGVVGWV KNTSKGTVTG QVQGPEEKVN 70 80 90 SMKSWLSKVG SPSSRIDRTN FSNEKTISKL EYSNFSVRY

References

[1]	"The complete amino acid sequence of horse muscle acylphosphatase." Cappugi G., Manao G., Camici G., Ramponi G. J. Biol. Chem. 255:6868-6874(1980) [PubMed: 6248536] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-99. Tissue: Muscle.
[2]	"Identification and description of alpha-helical regions in horse muscle acylphosphatase by 1H nuclear magnetic resonance spectroscopy." Saudek V., Atkinson R.A., Williams R.J.P., Ramponi G. J. Mol. Biol. 205:229-239(1989) [PubMed: 2538623] [Abstract] Cited for: STRUCTURE BY NMR OF ALPHA HELICES.
[3]	"Identification and description of beta-structure in horse muscle acylphosphatase by nuclear magnetic resonance spectroscopy." Saudek V., Wormald M.R., Williams R.J.P., Boyd J., Stefani M., Ramponi G. J. Mol. Biol. 207:405-415(1989) [PubMed: 2547076] [Abstract] Cited for: STRUCTURE BY NMR OF BETA-STRUCTURES.
[4]	"Three-dimensional structure of acylphosphatase. Refinement and structure analysis." Pastore A., Saudek V., Ramponi G., Williams R.J.P. J. Mol. Biol. 224:427-440(1992) [PubMed: 1313885] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

PIR

QPHO. A01015.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1APS	NMR	-	A	1-99	[»]

ModBase

Search...

Phylogenomic databases

HOVERGEN

P00818.

Family and domain databases

InterPro

IPR001792. Acylphosphatase.
[Graphical view]

PANTHER

PTHR10029. Acylphosphatase. 1 hit.

Pfam

PF00708. Acylphosphatase. 1 hit.
[Graphical view]

PRINTS

PR00112. ACYLPHPHTASE.

ProDom

PD001884. Acylphosphatase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00150. ACYLPHOSPHATASE_1. 1 hit.
PS00151. ACYLPHOSPHATASE_2. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P00818.

Entry information

Entry name

ACYP2_HORSE

Accession

Primary (citable) accession number: P00818

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)