Reviewed, UniProtKB/Swiss-Prot P01179 (NEU1_RAT)

Names and origin

Protein names

Recommended name:
    Oxytocin-neurophysin 1
      Short name=OT-NPI
Cleaved into the following 2 chains:
    1- Recommended name:
            Oxytocin
        Alternative name(s):
            Ocytocin
    2- Recommended name:
            Neurophysin 1

Gene names

Name:	Oxt
Synonyms:	Ot

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	125 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.
Sequence similarities	Belongs to the vasopressin/oxytocin family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 19	19
	Peptide	20 – 28	9	Oxytocin
	Chain	32 – 125	94	Neurophysin 1
Amino acid modifications
	Modified residue	28	1	Glycine amide
	Disulfide bond	20 ↔ 25
	Disulfide bond	41 ↔ 85		By similarity
	Disulfide bond	44 ↔ 58		By similarity
	Disulfide bond	52 ↔ 75		By similarity
	Disulfide bond	59 ↔ 65		By similarity
	Disulfide bond	92 ↔ 104		By similarity
	Disulfide bond	98 ↔ 116		By similarity
	Disulfide bond	105 ↔ 110		By similarity
Experimental info
	Sequence conflict	100	1	T → A AA sequence Ref.6
	Sequence conflict	124 – 125	2	ER → Q AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P01179-1 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: DC98F90BADC5551F	FASTA	125	12,823
	10 20 30 40 50 60 MACPSLACCL LGLLALTSAC YIQNCPLGGK RAALDLDMRK CLPCGPGGKG RCFGPSICCA 70 80 90 100 110 120 DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAT AGICCSPDGC RTDPACDPES AFSER

References

[1]	"Structure and comparison of the oxytocin and vasopressin genes from rat." Ivell R., Richter D. Proc. Natl. Acad. Sci. U.S.A. 81:2006-2010(1984) [PubMed: 6326097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The neurohypophyseal hormones vasopressin and oxytocin. Precursor structure, synthesis and regulation." Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H., Richter D. Biol. Chem. Hoppe-Seyler 367:695-704(1986) [PubMed: 3768139] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Expression of the vasopressin and oxytocin genes in rats occurs in mutually exclusive sets of hypothalamic neurons." Mohr E., Bahnsen U., Kiessling C., Richter D. FEBS Lett. 242:144-148(1988) [PubMed: 3203740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Rat vasopressin and oxytocin genes are linked by a long interspersed repeated DNA element (LINE): sequence and transcriptional analysis of LINE." Schmitz E., Mohr E., Richter D. DNA Cell Biol. 10:81-91(1991) [PubMed: 1706187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar. Tissue: Liver.
[5]	"Identification of rat neurophysins: complete amino acid sequences of MSEL- and VLDV-neurophysins." Chauvet M.-T., Chauvet J., Acher R. Biochem. Biophys. Res. Commun. 103:595-603(1981) [PubMed: 7036996] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-125.
[6]	"Evolution of neurophysin proteins: partial amino acid sequences of rat neurophysins." North W.G., Mitchell T.I. FEBS Lett. 126:41-44(1981) [PubMed: 7238864] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-100.
[7]	"A comparative study of partial neurophysin protein sequences of cod, guinea pig, rat and sheep." Schlesinger D.H., Pickering B.T., Watkins W.B., Peek J.C., Moore L.G., Audhya T.K., Walter R. FEBS Lett. 80:371-373(1977) [PubMed: 891987] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-64.
[8]	"Evolution of neurohypophyseal hormones: isolation of active principles from rabbits and rats." Chauvet J., Chauvet M.-T., Acher R. Biochimie 53:1099-1104(1971) [PubMed: 5150741] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-28.

Cross-references

Sequence databases
EMBLGenBankDDBJ	K01701 Genomic DNA. Translation: AAA98733.1. M25649 mRNA. Translation: AAA41773.1. X12792 Genomic DNA. Translation: CAA31281.1. X59496 Genomic DNA. Translation: CAA42085.1.
PIR	NFRT1. A21183.
RefSeq	NP_037128.1.
UniGene	Rn.48915
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000021225. Rattus norvegicus. [Contig view]
GeneID	25504.
KEGG	rno:25504.
Organism-specific databases
RGD	3238. Oxt.
Phylogenomic databases
HOVERGEN	P01179.
Gene expression databases
ArrayExpress	P01179.
GermOnline	ENSRNOG00000021225. Rattus norvegicus.
Family and domain databases
InterPro	IPR000981. Neurhyp_horm. IPR016321. Nonapeptide_hormone_prcur. [Graphical view]
Gene3D	G3DSA:2.60.9.10. Neurhyp_horm. 1 hit.
Pfam	PF00220. Hormone_4. 1 hit. PF00184. Hormone_5. 1 hit. [Graphical view]
PIRSF	PIRSF001815. Nonapeptide_hormone_precursor. 1 hit.
PRINTS	PR00831. NEUROPHYSIN.
ProDom	PD001676. Neurhyp_horm. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00003. NH. 1 hit. [Graphical view]
PROSITE	PS00264. NEUROHYPOPHYS_HORM. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	P01179.
ProtoNet	Search...

Entry information

Entry name

NEU1_RAT

Accession

Primary (citable) accession number: P01179
Secondary accession number(s): Q53WU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 1, 1989
Last modified:	July 22, 2008
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)