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Reviewed, UniProtKB/Swiss-Prot P02557 (TBB_YEAST)

Last modified September 2, 2008. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Tubulin beta chain
Alternative name(s):
    Beta-tubulin
Gene names
Name: TUB2
Ordered Locus Names: YFL037W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Subunit structure

Dimer of alpha and beta chains.

Miscellaneous

Rhizoxin, an antibiotic that exhibits potent anti-mitotic activity against most eukaryotic cells except for yeasts, binds to beta tubulin.

Sequence similarities

Belongs to the tubulin family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

RBL2P486061EBI-18986,EBI-18991

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 457457Tubulin beta chain

Regions

Nucleotide binding140 – 1467GTP Potential

Amino acid modifications

Modified residue2781Phosphoserine
Modified residue2801Phosphoserine

Experimental info

Mutagenesis1001V → N: Becomes sensitive to rhizoxin
Sequence conflict91T → A in CAA24603. Ref.1
Sequence conflict121C → Y in CAA24603. Ref.1
Sequence conflict711G → W in CAA24603. Ref.1
Sequence conflict1521I → F in CAA24603. Ref.1
Sequence conflict1561R → K in CAA24603. Ref.1
Sequence conflict1591F → L in CAA24603. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P02557-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 68EBEA7D7A5B8EA1

FASTA45750,923
        10         20         30         40         50         60 
MREIIHISTG QCGNQIGAAF WETICGEHGL DFNGTYHGHD DIQKERLNVY FNEASSGKWV 

        70         80         90        100        110        120 
PRSINVDLEP GTIDAVRNSA IGNLFRPDNY IFGQSSAGNV WAKGHYTEGA ELVDSVMDVI 

       130        140        150        160        170        180 
RREAEGCDSL QGFQITHSLG GGTGSGMGTL LISKIREEFP DRMMATFSVL PSPKTSDTVV 

       190        200        210        220        230        240 
EPYNATLSVH QLVEHSDETF CIDNEALYDI CQRTLKLNQP SYGDLNNLVS SVMSGVTTSL 

       250        260        270        280        290        300 
RYPGQLNSDL RKLAVNLVPF PRLHFFMVGY APLTAIGSQS FRSLTVPELT QQMFDAKNMM 

       310        320        330        340        350        360 
AAADPRNGRY LTVAAFFRGK VSVKEVEDEM HKVQSKNSDY FVEWIPNNVQ TAVCSVAPQG 

       370        380        390        400        410        420 
LDMAATFIAN STSIQELFKR VGDQFSAMFK RKAFLHWYTS EGMDELEFSE AESNMNDLVS 

       430        440        450 
EYQQYQEATV EDDEEVDENG DFGAPQNQDE PITENFE

« Hide

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References

« Hide 'large scale' references
[1]"Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo."
Neff N.F., Thomas J.H., Grisafi P., Botstein D.
Cell 33:211-219(1983) [PubMed: 6380751] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product."
Gallwitz D., Donath C., Sander C.
Nature 306:704-707(1983) [PubMed: 6318115] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
[4]"Molecular basis for determining the sensitivity of eucaryotes to the antimitotic drug rhizoxin."
Takahashi M., Matsumoto S., Iwasaki S., Yahara I.
Mol. Gen. Genet. 222:169-175(1990) [PubMed: 2274023] [Abstract]
Cited for: ANTIBIOTIC RESISTANCE TO RHIZOXIN.
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278 AND SER-280, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

V01296 Genomic DNA. Translation: CAA24603.1.
D50617 Genomic DNA. Translation: BAA09202.1.
X00209 Genomic DNA. Translation: CAA25035.1.
PIRUBBYB. S56217.
RefSeqNP_116616.1.

3D structure databases

HSSPHSSP built from PDB template 1FFX based on UniProtKB P02554.
SMRP02557. Positions 2-427.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2340N.
IntActP02557.

Proteomic databases

PeptideAtlasP02557.

Genome annotation databases

EnsemblYFL037W. Saccharomyces cerevisiae. [Contig view]
GeneID850506.
GenomeReviewsGene locus YFL037W in contig D50617_GR.
KEGGsce:YFL037W.
NMPDRfig|4932.3.peg.2241.

Organism-specific databases

CYGDYFL037w.
SGDS000001857. TUB2.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP02557.

Gene expression databases

ArrayExpressP02557.
GermOnlineYFL037W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR000158. Cell_division_FtsZ_N.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR003008. Tubulin_FtsZ.
[Graphical view]
Gene3DG3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
PANTHERPTHR11588:SF9. Beta_tubulin. 1 hit.
PTHR11588. Tubulin. 1 hit.
PfamPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSPR01163. BETATUBULIN.
PR00423. CELLDVISFTSZ.
PR01161. TUBULIN.
PROSITEPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]
BLOCKSSearch...

Other Resources

LinkHubP02557.
ProtoNetSearch...

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Entry information

Entry nameTBB_YEAST
AccessionPrimary (citable) accession number: P02557
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: September 2, 2008
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents