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Reviewed, UniProtKB/Swiss-Prot P02930 (TOLC_ECOLI)

Last modified June 10, 2008. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesOuter membrane protein tolC [Precursor]
Gene names
Name: tolC
Synonyms: mtcB, mukA, refI
Ordered Locus Names: b3035, JW5503
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria.

Subunit structure

Homotrimer that assembles to form a continuous, solvent-accessible conduit: a 'channel-tunnel' over 140 Angstroms long that spans both the outer membrane and periplasmic space. The periplasmic or proximal end of the tunnel is sealed by sets of coiled helices.

Subcellular location

Cell outer membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the outer membrane factor (OMF) (TC 1.B.17) family. [View classification]

Sequence caution

CAA24914.1 sequence differs from that shown. Reason: Miscellaneous discrepancy.

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Ontologies

Keywords

   Biological processTransport
   Cellular componentMembrane
Outer membrane
   DomainRepeat
Signal
Transmembrane
   Technical term3D-structure
Complete proteome
Direct protein sequencing

Gene Ontology (GO)

   Cellular componentcell outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Signal peptide1 – 2222
Chain23 – 493471Outer membrane protein tolC

Regions

Topological domain23 – 6240Periplasmic
Transmembrane63 – 7412S1
Topological domain75 – 828Extracellular
Transmembrane83 – 9614S2
Topological domain97 – 268172Periplasmic
Transmembrane269 – 27911S4
Topological domain280 – 30021Extracellular
Transmembrane301 – 31111S5
Topological domain312 – 493182Periplasmic
Repeat23 – 2302081
Repeat231 – 4462162

Experimental info

Sequence conflict1911V → L in CAA37982. Ref.1

Secondary structure

1 ........................................... 493
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P02930-1 [UniParc].

Last modified June 27, 2006. Version 3.
Checksum: 6F97B4C62A848FE1

FASTA49353,741
        10         20         30         40         50         60 
MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF EKINEARSPL 

        70         80         90        100        110        120 
LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM SKWRALTLQE KAAGIQDVTY 

       130        140        150        160        170        180 
QTDQQTLILN TATAYFNVLN AIDVLSYTQA QKEAIYRQLD QTTQRFNVGL VAITDVQNAR 

       190        200        210        220        230        240 
AQYDTVLANE VTARNNLDNA VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK 

       250        260        270        280        290        300 
RNLSLLQARL SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN 

       310        320        330        340        350        360 
MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR SSFNNINASI 

       370        380        390        400        410        420 
SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT LYNAKQELAN ARYNYLINQL 

       430        440        450        460        470        480 
NIKSALGTLN EQDLLALNNA LSKPVSTNPE NVAPQTPEQN AIADGYAPDS PAPVVQQTSA 

       490 
RTTTSNGHNP FRN

« Hide

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References

« Hide 'large scale' references
[1]"Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12."
Hackett J., Reeves P.
Nucleic Acids Res. 11:6487-6495(1983) [PubMed: 6312426] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the tolC gene of Escherichia coli."
Niki H., Imamura R., Ogura T., Hiraga S.
Nucleic Acids Res. 18:5547-5547(1990) [PubMed: 2216730] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The TolC protein of Escherichia coli K12 is synthesised in a precursor form."
Hackett J., Misra R., Reeves P.
FEBS Lett. 156:307-310(1983) [PubMed: 6303857] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45.
Strain: K12.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-34.
Strain: K12 / EMG2.
[7]"Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis."
Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.-C., Hochstrasser D.F., Williams K.L., Gooley A.A.
Electrophoresis 19:837-844(1998) [PubMed: 9629924] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-27.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]"Protein complexes of the Escherichia coli cell envelope."
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
Strain: BL21-DE3.
[9]"Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export."
Koronakis V., Sharff A., Koronakis E., Luisi B., Hughes C.
Nature 405:914-919(2000) [PubMed: 10879525] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-450.

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Cross-references

Sequence databases

X00016 Genomic DNA. Translation: CAA24914.1. Sequence problems.
X54049 Genomic DNA. Translation: CAA37982.1. Different initiation.
U28377 Genomic DNA. Translation: AAA69203.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76071.2.
AP009048 Genomic DNA. Translation: BAE77091.1.
V01505 Genomic DNA. Translation: CAA24751.1. Different initiation.
PIRMMECTC. A65091.
RefSeqAP_003585.1.
NP_417507.2.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1EK9X-ray2.10A/B/C23-450[»]
1TQQX-ray2.75A/B/C23-493[»]
1TTQX-ray2.00A-[»]
B1-12[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:11007N.

2-D gel databases

SWISS-2DPAGEP02930.
2DBase-EcoliP02930.

Genome annotation databases

GeneID947521.
GenomeReviewsGene locus b3035 in contig U00096_GR.
Gene locus JW5503 in contig AP009048_GR.
KEGGecj:JW5503.
eco:b3035.

Organism-specific databases

EchoBASEEB1002.
EcoGeneEG11009. tolC.
CMRSearch...

Phylogenomic databases

HOGENOMP02930.

Enzyme and pathway databases

BioCycEcoCyc:EG11009-MON.

Family and domain databases

InterProIPR003423. OMP_efflux.
IPR010130. T1SS_OMP_TolC.
[Graphical view]
PfamPF02321. OEP. 2 hits.
[Graphical view]
TIGRFAMsTIGR01844. type_I_sec_TolC. 1 hit.
ProDomP02930.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

LinkHubP02930.
ProtoNetSearch...

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Entry information

Entry nameTOLC_ECOLI
AccessionPrimary (citable) accession number: P02930
Secondary accession number(s): Q2M9G5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 27, 2006
Last modified: June 10, 2008
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Recent format changes

Overview of recent format changes

User manual

User manual for UniProtKB

Names and origin · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents