Reviewed, UniProtKB/Swiss-Prot P04037 (COX4_YEAST)

Names and origin

Protein names

Recommended name:
    Cytochrome c oxidase polypeptide 4, mitochondrial
    EC=1.9.3.1
Alternative name(s):
    Cytochrome c oxidase polypeptide IV

Gene names

Name:	COX4
Ordered Locus Names:	YGL187C
ORF Names:	G1362

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	4 ferrocytochrome c + O(2) + 4 H(+) = 4 ferricytochrome c + 2 H(2)O.
Subunit structure	Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 9410 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cytochrome c oxidase Vb family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from physical interaction. Source: SGD mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay. Source: SGD
Cellular component	mitochondrial respiratory chain complex IV Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKI8	Q02793	1	EBI-5039,EBI-17260

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Transit peptide

1 – 25

25

Mitochondrion

Chain

26 – 155

130

Cytochrome c oxidase polypeptide 4, mitochondrial

Sites

Metal binding

111

1

Zinc Potential

Metal binding

134

1

Zinc Potential

Metal binding

137

1

Zinc Potential

Amino acid modifications

Modified residue

55

1

Phosphothreonine

Experimental info

Sequence conflict

26

1

Q → E AA sequence Ref.6

Sequence conflict

73

1

G → C AA sequence Ref.6

Secondary structure

1

.

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.

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155

Helix Strand Turn

Details...

Beta strand

15 – 17

3

Beta strand

98 – 100

3

Beta strand

103 – 106

4

Beta strand

109 – 114

6

Beta strand

122 – 124

3

Beta strand

131 – 133

3

Beta strand

135 – 137

3

Beta strand

140 – 143

4

Sequences

	Sequence		Length	Mass (Da)
	P04037-1 [UniParc]. Last modified November 1, 1986. Version 1. Checksum: AB2EE69878819E0F	FASTA	155	17,143
	10 20 30 40 50 60 MLSLRQSIRF FKPATRTLCS SRYLLQQKPV VKTAQNLAEV NGPETLIGPG AKEGTVPTDL 70 80 90 100 110 120 DQETGLARLE LLGKLEGIDV FDTKPLDSSR KGTMKDPIII ESYDDYRYVG CTGSPAGSHT 130 140 150 IMWLKPTVNE VARCWECGSV YKLNPVGVPN DDHHH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein." Maarse A.C., van Loon A.P.G.M., Riezman H., Gregor I., Schatz G., Grivell L.A. EMBO J. 3:2831-2837(1984) [PubMed: 6098449] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed: 17244705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-33. Strain: ATCC 201390 / BY4743.
[5]	"The untranslated leader of nuclear COX4 gene of Saccharomyces cerevisiae contains an intron." Schneider J.C., Guarente L. Nucleic Acids Res. 15:3515-3529(1987) [PubMed: 3033605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
[6]	"The nuclear-coded subunits of yeast cytochrome c oxidase. III. Identification of homologous subunits in yeast, bovine heart, and Neurospora crassa cytochrome c oxidases." Power S.D., Lochrie M.A., Poyton R.O. J. Biol. Chem. 259:6575-6578(1984) [PubMed: 6327686] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-79.
[7]	"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method." Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G. Eur. J. Biochem. 227:296-302(1995) [PubMed: 7851399] [Abstract] Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
[8]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-55, MASS SPECTROMETRY.
[9]	"Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences." Taylor A.B., Smith B.S., Kitada S., Kojima K., Miyaura H., Otwinowski Z., Ito A., Deisenhofer J. Structure 9:615-625(2001) [PubMed: 11470436] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-25 IN COMPLEX WITH MAS1/MAS2 HETERODIMER.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X01418 Genomic DNA. Translation: CAA25665.1.
X91489 Genomic DNA. Translation: CAA62787.1.
Z72709 Genomic DNA. Translation: CAA96899.1.
EF123139 mRNA. Translation: ABM97483.1.
Y00152 Genomic DNA. Translation: CAA68347.1.

PIR

OLBY4. A22786.

RefSeq

NP_011328.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HR8	X-ray	2.70	O/P/Q/R	2-25	[»]
2ODX	NMR	-	A	79-155	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2763N.

IntAct

P04037.

Proteomic databases

PeptideAtlas

P04037.

Genome annotation databases

Ensembl

YGL187C. Saccharomyces cerevisiae. [Contig view]

GeneID

852688.

GenomeReviews

Gene locus YGL187C in contig Y13135_GR.

KEGG

sce:YGL187C.

NMPDR

fig|4932.3.peg.2432.

Organism-specific databases

CYGD

YGL187c.

SGD

S000003155. COX4.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P04037.

Gene expression databases

GermOnline

YGL187C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002124. COX5B.
[Graphical view]

Gene3D

G3DSA:2.60.11.10. COX5B. 1 hit.

PANTHER

PTHR10122. COX5B. 1 hit.

Pfam

PF01215. COX5B. 1 hit.
[Graphical view]

ProDom

PD007270. COX5B. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00848. COX5B_1. 1 hit.
PS51359. COX5B_2. 1 hit.
[Graphical view]

BLOCKS

Search...

Other Resources

LinkHub

P04037.

ProtoNet

Search...

Entry information

Entry name

COX4_YEAST

Accession

Primary (citable) accession number: P04037
Secondary accession number(s): A2TBN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	November 1, 1986
Last modified:	July 22, 2008
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)