Reviewed, UniProtKB/Swiss-Prot P04733 (MT1F_HUMAN)

Names and origin

Protein names

Recommended name:
    Metallothionein-1F
      Short name=MT-1F
Alternative name(s):
    Metallothionein-IF
      Short name=MT-IF

Gene names

Name:	MT1F
ORF Names:	PRO0376

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Subunit structure	Monomer.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Cadmium Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Non-traceable author statement. Source: UniProtKB
Molecular function	cadmium ion binding Non-traceable author statement. Source: UniProtKB copper ion binding Non-traceable author statement. Source: UniProtKB zinc ion binding Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 61	61	Metallothionein-1F
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	13	1	Cluster B
	Metal binding	15	1	Cluster B
	Metal binding	19	1	Cluster B
	Metal binding	21	1	Cluster B
	Metal binding	24	1	Cluster B
	Metal binding	26	1	Cluster B
	Metal binding	29	1	Cluster B
	Metal binding	33	1	Cluster A
	Metal binding	34	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	41	1	Cluster A
	Metal binding	44	1	Cluster A
	Metal binding	48	1	Cluster A
	Metal binding	50	1	Cluster A
	Metal binding	57	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	60	1	Cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P04733-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 7C7A61E180D8D1C8	FASTA	61	6,086
	10 20 30 40 50 60 MDPNCSCAAG VSCTCAGSCK CKECKCTSCK KSCCSCCPVG CSKCAQGCVC KGASEKCSCC D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of two human metallothionein-I isoform genes and a related pseudogene." Schmidt C.J., Jubier M.-F., Hamer D.H. J. Biol. Chem. 260:7731-7737(1985) [PubMed: 2581970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure, organization, and regulation of human metallothionein IF gene: differential and cell-type-specific expression in response to heavy metals and glucocorticoids." Varshney U., Jahroudi N., Foster R., Gedamu L. Mol. Cell. Biol. 6:26-37(1986) [PubMed: 3023827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structure and expression of the human metallothionein genes." Gedamu L., Varshney U., Jahroudi N., Foster R., Shworak N.W. Experientia Suppl. 52:361-372(1987) [PubMed: 2444457] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Functional prediction of the coding sequences of 50 new genes deduced by analysis of cDNA clones from human fetal liver." Yu Y., Zhang C., Luo L., Ouyang S., Zhang S., Li W., Wu J., Zhou S., Liu M., He F. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal liver.
[5]	Hunziker P.E. Submitted (NOV-1993) to UniProtKB Cited for: PROTEIN SEQUENCE. Tissue: Liver.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M10943 Genomic DNA. Translation: AAA59588.1. M13003 Genomic DNA. Translation: AAA36213.1. AF078844 mRNA. Translation: AAF23355.1. Different initiation. BC029453 mRNA. Translation: AAH29453.1.
PIR	SMHU1F. B22634.
RefSeq	NP_005940.1.
UniGene	Hs.513626
3D structure databases
HSSP	HSSP built from PDB template 2MHU based on UniProtKB P02795.
SMR	P04733. Positions 1-60.
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000198417. Homo sapiens. [Contig view]
GeneID	4494.
KEGG	hsa:4494.
Organism-specific databases
H-InvDB	HIX0043884. HIX0059560.
HGNC	HGNC:7398. MT1F.
MIM	156352. gene.
PharmGKB	PA31203.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P04733.
HOVERGEN	P04733.
Gene expression databases
ArrayExpress	P04733.
CleanEx	HS_MT1F.
GermOnline	ENSG00000198417. Homo sapiens.
Family and domain databases
InterPro	IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProDom	P04733. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P04733.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

MT1F_HUMAN

Accession

Primary (citable) accession number: P04733
Secondary accession number(s): Q9UI97

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	July 22, 2008
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)