Reviewed, UniProtKB/Swiss-Prot P05301 (T2D1_DESNO)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme DdeI
      Short name=R.DdeI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme DdeI
    Endonuclease DdeI

Gene names

Name:	ddeIR
Synonyms:	ddeR

Organism

Desulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))

Taxonomic identifier

52561 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfomicrobiaceae › Desulfomicrobium

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTNAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 240	240	Type-2 restriction enzyme DdeI

Sequences

	Sequence		Length	Mass (Da)
	P05301-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: AE0A7821F2F41356	FASTA	240	27,808
	10 20 30 40 50 60 MKAATDQELR KLIVLYNNVM EVMEHDAAKS MRDDNRAYGG FVRAAKGKIQ ELITERLVRT 70 80 90 100 110 120 VWDVEMGENP ERLSINSKKI KIPILRSYVD SINDENLKKY ISSNILKYSY GLSVDKHVFI 130 140 150 160 170 180 DNKFVLGIEC KAYTENAMLK RILVDFYLLK TKFPKLNCFL FQLESQLGGD YSECNKFPIG 190 200 210 220 230 240 SYPTRTIMSY FKNVDLNIVT LLEGERKVDR PINKPQFFKP LKVEHLEVAI GYLQESLSEI

References

[1]	"Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein." Sznyter L.A., Slatko B., Moran L., O'Donnell K.H., Brooks J.E. Nucleic Acids Res. 15:8249-8266(1987) [PubMed: 2823226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00449 Genomic DNA. Translation: CAA68504.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	770. DdeI.
Family and domain databases
ProDom	P05301. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

T2D1_DESNO

Accession

Primary (citable) accession number: P05301

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	September 2, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)