Reviewed, UniProtKB/Swiss-Prot P13470 (GTFC_STRMU)

Names and origin

Protein names

Recommended name:
    Glucosyltransferase-SI
      Short name=GTF-SI
    EC=2.4.1.5
Alternative name(s):
    Dextransucrase
    Sucrose 6-glucosyltransferase

Gene names

Name:	gtfC
Ordered Locus Names:	SMU_1005

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
Catalytic activity	Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).
Subcellular location	Secreted.
Miscellaneous	GTF-I synthesizes water-insoluble glucans (alpha 1,3-linked glucose and some 1,6 linkages), GTF-S synthesizes water-soluble glucans (alpha 1,6-glucose). GTF-SI synthesizes both forms of glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 70 family. Contains 11 cell wall-binding repeats.

Ontologies

Keywords
Cellular component	Secreted
Disease	Dental caries
Domain	Repeat Signal
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dextransucrase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34
	Chain	35 – 1455	1421	Glucosyltransferase-SI		PRO_0000021386
Regions
	Repeat	184 – 203	20	Cell wall-binding 1
	Repeat	205 – 224	20	Cell wall-binding 2
	Repeat	1116 – 1135	20	Cell wall-binding 3
	Repeat	1136 – 1155	20	Cell wall-binding 4
	Repeat	1219 – 1239	21	Cell wall-binding 5
	Repeat	1242 – 1262	21	Cell wall-binding 6
	Repeat	1263 – 1282	20	Cell wall-binding 7
	Repeat	1307 – 1327	21	Cell wall-binding 8
	Repeat	1328 – 1347	20	Cell wall-binding 9
	Repeat	1372 – 1392	21	Cell wall-binding 10
	Repeat	1393 – 1412	20	Cell wall-binding 11
	Region	250 – 1050	801	Catalytic; approximate
Natural variations
	Natural variant	21	1	V → I in strain: GS-5.
	Natural variant	81	1	P → L in strain: MT4239.
	Natural variant	106	1	D → V in strain: GS-5.
	Natural variant	116	1	S → A in strain: GS-5 and MT4467.
	Natural variant	126	1	A → T in strain: GS-5.
	Natural variant	150 – 151	2	SR → PK in strain: GS-5, MT4239 and MT4467.
	Natural variant	256	1	A → V in strain: GS-5 and MT4467.
	Natural variant	425	1	R → N in strain: MT4251.
	Natural variant	519	1	Y → D in strain: MT4245 and MT4251.
	Natural variant	538	1	R → K in strain: MT4245 and MT4251.
	Natural variant	545	1	Y → F in strain: MT4245 and MT4251.
	Natural variant	597	1	N → D in strain: MT4245, MT4251, MT4467 and MT8148.
	Natural variant	600	1	R → K in strain: MT4245, MT4251, MT4467 and MT8148.
	Natural variant	601	1	A → T in strain: GS-5.
	Natural variant	614	1	M → T in strain: GS-5.
	Natural variant	727	1	T → I in strain: MT8148.
	Natural variant	734	1	A → V in strain: MT8148.
	Natural variant	964	1	L → F in strain: MT4239.
	Natural variant	1113	1	N → Y in strain: MT4239.
	Natural variant	1118	1	A → T in strain: MT4239.
	Natural variant	1204	1	I → V in strain: GS-5, MT4239, MT4467 and MT8148.
	Natural variant	1208	1	V → I in strain: MT8148.
	Natural variant	1292 – 1294	3	DGH → NGY in strain: GS-5, MT4467 and sMT8148.
	Natural variant	1305 – 1369	65	Missing in strain: MT4245.
	Natural variant	1326	1	I → V in strain: MT8148.
	Natural variant	1331	1	T → A in strain: GS-5, MT4239, MT4467 and MT8148.
	Natural variant	1377	1	R → K in strain: MT8148.
	Natural variant	1398	1	V → I in strain: MT8148.
	Natural variant	1424	1	D → N in strain: MT4239.
	Natural variant	1439	1	V → I in strain: MT4239 and MT8148.
	Natural variant	1444	1	S → P in strain: MT8148.
Experimental info
	Sequence conflict	1337 – 1455	119	QRLYF…NFFRF → HASILSLMVFRLRESSLQSV KVVSNTMILIPEMKFVIVM in AAA88592 and BAA26102. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13470-1 [UniParc]. Last modified November 28, 2002. Version 2. Checksum: 3CB455A99A4FEC86	FASTA	1,455	162,966
	10 20 30 40 50 60 MEKKVRFKLR KVKKRWVTVS VASAVVTLTS LSGSLVKADS TDDRQQAVTE SQASLVTTSE 70 80 90 100 110 120 AAKETLTATD TSTATSATSQ PTATVTDNVS TTNQSTNTTA NTANFDVKPT TTSEQSKTDN 130 140 150 160 170 180 SDKIIATSKA VNRLTATGKF VPANNNTAHS RTVTDKIVPI KPKIGKLKQP SSLSQDDIAA 190 200 210 220 230 240 LGNVKNIRKV NGKYYYYKED GTLQKNYALN INGKTFFFDE TGALSNNTLP SKKGNITNND 250 260 270 280 290 300 NTNSFAQYNQ VYSTDAANFE HVDHYLTAES WYRPKYILKD GKTWTQSTEK DFRPLLMTWW 310 320 330 340 350 360 PDQETQRQYV NYMNAQLGIH QTYNTATSPL QLNLAAQTIQ TKIEEKITAE KNTNWLRQTI 370 380 390 400 410 420 SAFVKTQSAW NSDSEKPFDD HLQKGALLYS NNSKLTSQAN SNYRILNRTP TNQTGKKDPR 430 440 450 460 470 480 YTADRTIGGY EFLLANDVDN SNPVVQAEQL NWLHFLMNFG NIYANDPDAN FDSIRVDAVD 490 500 510 520 530 540 NVDADLLQIA GDYLKAAKGI HKNDKAANDH LSILEAWSYN DTPYLHDDGD NMINMDNRLR 550 560 570 580 590 600 LSLLYSLAKP LNQRSGMNPL ITNSLVNRTD DNAETAAVPS YSFIRAHDSE VQDLIRNIIR 610 620 630 640 650 660 AEINPNVVGY SFTMEEIKKA FEIYNKDLLA TEKKYTHYNT ALSYALLLTN KSSVPRVYYG 670 680 690 700 710 720 DMFTDDGQYM AHKTINYEAI ETLLKARIKY VSGGQAMRNQ QVGNSEIITS VRYGKGALKA 730 740 750 760 770 780 TDTGDRTTRT SGVAVIEGNN PSLRLKASDR VVVNMGAAHK NQAYRPLLLT TDNGIKAYHS 790 800 810 820 830 840 DQEAAGLVRY TNDRGELIFT AADIKGYANP QVSGYLGVWV PVGAAADQDV RVAASTAPST 850 860 870 880 890 900 DGKSVHQNAA LDSRVMFEGF SNFQAFATKK EEYTNVVIAK NVDKFAEWGV TDFEMAPQYV 910 920 930 940 950 960 SSTDGSFLDS VIQNGYAFTD RYDLGISKPN KYGTADDLVK AIKALHSKGI KVMADWVPDQ 970 980 990 1000 1010 1020 MYALPEKEVV TATRVDKYGT PVAGSQIKNT LYVVDGKSSG KDQQAKYGGA FLEELQAKYP 1030 1040 1050 1060 1070 1080 ELFARKQIST GVPMDPSVKI KQWSAKYFNG TNILGRGAGY VLKDQATNTY FSLVSDNTFL 1090 1100 1110 1120 1130 1140 PKSLVNPNHG TSSSVTGLVF DGKGYVYYST SGNQAKNAFI SLGNNWYYFD NNGYMVTGAQ 1150 1160 1170 1180 1190 1200 SINGANYYFL SNGIQLRNAI YDNGNKVLSY YGNDGRRYEN GYYLFGQQWR YFQNGIMAVG 1210 1220 1230 1240 1250 1260 LTRIHGAVQY FDASGFQAKG QFITTADGKL RYFDRDSGNQ ISNRFVRNSK GEWFLFDHNG 1270 1280 1290 1300 1310 1320 VAVTGTVTFN GQRLYFKPNG VQAKGEFIRD ADGHLRYYDP NSGNEVRNRF VRNSKGEWFL 1330 1340 1350 1360 1370 1380 FDHNGIAVTG TRVVNGQRLY FKSNGVQAKG ELITERKGRI KYYDPNSGNE VRNRYVRTSS 1390 1400 1410 1420 1430 1440 GNWYYFGNDG YALIGWHVVE GRRVYFDENG VYRYASHDQR NHWDYDYRRD FGRGSSSAVR 1450 FRHSRNGFFD NFFRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the gtfC gene from Streptococcus mutans GS-5." Ueda S., Shiroza T., Kuramitsu H.K. Gene 69:101-109(1988) [PubMed: 2976010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GS-5.
[2]	"Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans." Fujiwara T., Terao Y., Hoshino T., Kawabata S., Ooshima T., Sobue S., Kimura S., Hamada S. FEMS Microbiol. Lett. 161:331-336(1998) [PubMed: 9570124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MT4239 / Serotype c, MT4245 / Serotype e, MT4251 / Serotype f, MT4467 / Serotype e and MT8148 / Serotype c.
[3]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.
[4]	"Sequence analysis of the gtfB gene from Streptococcus mutans." Shiroza T., Ueda S., Kuramitsu H.K. J. Bacteriol. 169:4263-4270(1987) [PubMed: 3040685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-349. Strain: GS-5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M22054 Genomic DNA. Translation: AAA88592.1. D88652 Genomic DNA. Translation: BAA26102.1. D88655 Genomic DNA. Translation: BAA26106.1. D88658 Genomic DNA. Translation: BAA26110.1. D88661 Genomic DNA. Translation: BAA26114.1. D89978 Genomic DNA. Translation: BAA26120.1. AE014133 Genomic DNA. Translation: AAN58706.1. M17361 Genomic DNA. Translation: AAA88589.1.
PIR	JT0345.
RefSeq	NP_721400.1.
3D structure databases
HSSP	HSSP built from PDB template 1H8G based on UniProtKB P06653.
ModBase	Search...
Genome annotation databases
GeneID	1028343.
GenomeReviews	Gene locus SMU_1005 in contig AE014133_GR.
KEGG	smu:SMU.1005.
NMPDR	fig|210007.1.peg.913.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_1005-MON.
Family and domain databases
InterPro	IPR002479. Cell_wall_bd_put. IPR003318. Glyco_hydro70cat. IPR013781. Glyco_hydro_sub_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
Pfam	PF01473. CW_binding_1. 2 hits. PF02324. Glyco_hydro_70. 1 hit. [Graphical view]
PROSITE	PS51170. CW. 11 hits. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

GTFC_STRMU

Accession

Primary (citable) accession number: P13470
Secondary accession number(s): O69382 , O69385, O69388, O69391, O69397, P05427

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 28, 2002
Last modified:	November 4, 2008
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)