Reviewed, UniProtKB/Swiss-Prot P05480 (SRC_MOUSE)

Names and origin

Protein names

Neuronal proto-oncogene tyrosine-protein kinase Src
Also known as:
     EC 2.7.10.2
     pp60c-src
     p60-Src
     c-Src

Gene names

Name:

Src

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein existence

Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2 By similarity. Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin By similarity. Interacts with RALGPS1 via its SH3 domain By similarity.
Post-translational modification	Phosphorylated on Tyr-535 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Disease	Proto-oncogene
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Phosphoprotein
Gene Ontology (GO)
Biological process	cell migration Inferred from mutant phenotype. Source: MGI forebrain development Inferred from genetic interaction. Source: MGI peptidyl-tyrosine phosphorylation Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein tyrosine kinase activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Csf1	P07141	2	EBI-298680,EBI-777188
ena	Q8T4F7	2	EBI-298680,EBI-466810	From a different organism.
Ephb2	P54763	1	EBI-298680,EBI-537711

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 541	540	Neuronal proto-oncogene tyrosine-protein kinase Src
Regions
	Domain	83 – 150	68	SH3
	Domain	156 – 253	98	SH2
	Domain	275 – 528	254	Protein kinase
	Nucleotide binding	281 – 289	9	ATP By similarity
Sites
	Active site	394	1	Proton acceptor By similarity
	Binding site	303	1	ATP By similarity
Amino acid modifications
	Modified residue	17	1	Phosphoserine By similarity
	Modified residue	68	1	Phosphoserine By similarity
	Modified residue	73	1	Phosphothreonine By similarity
	Modified residue	74	1	Phosphoserine
	Modified residue	192	1	Phosphotyrosine
	Modified residue	424	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	535	1	Phosphotyrosine; by CSK By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05480-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 09BBA0EEF88A70B5	FASTA	541	60,619
	10 20 30 40 50 60 MGSNKSKPKD ASQRRRSLEP SENVHGAGGA FPASQTPSKP ASADGHRGPS AAFVPPAAEP 70 80 90 100 110 120 KLFGGFNSSD TVTSPQRAGA LAGGVTTFVA LYDYESRTET DLSFKKGERL QIVNNTRKVD 130 140 150 160 170 180 VREGDWWLAH SLSTGQTGYI PSNYVAPSDS IQAEEWYFGK ITRRESERLL LNAENPRGTF 190 200 210 220 230 240 LVRESETTKG AYCLSVSDFD NAKGLNVKHY KIRKLDSGGF YITSRTQFNS LQQLVAYYSK 250 260 270 280 290 300 HADGLCHRLT TVCPTSKPQT QGLAKDAWEI PRESLRLEVK LGQGCFGEVW MGTWNGTTRV 310 320 330 340 350 360 AIKTLKPGTM SPEAFLQEAQ VMKKLRHEKL VQLYAVVSEE PIYIVTEYMN KGSLLDFLKG 370 380 390 400 410 420 ETGKYLRLPQ LVDMSAQIAS GMAYVERMNY VHRDLRAANI LVGENLVCKV ADFGLARLIE 430 440 450 460 470 480 DNEYTARQGA KFPIKWTAPE AALYGRFTIK SDVWSFGILL TELTTKGRVP YPGMVNREVL 490 500 510 520 530 540 DQVERGYRMP CPPECPESLH DLMCQCWRKE PEERPTFEYL QAFLEDYFTS TEPQYQPGEN L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart." Martinez R., Mathey-Prevot B., Bernards A., Baltimore D. Science 237:411-415(1987) [PubMed: 2440106] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src." Brown M.T., Andrade J., Radhakrishna H., Donaldson J.G., Cooper J.A., Randazzo P.A. Mol. Cell. Biol. 18:7038-7051(1998) [PubMed: 9819391] [Abstract] Cited for: INTERACTION WITH DDEF1/ASAP1.
[3]	"Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs." Kostenko E.V., Olabisi O.O., Sahay S., Rodriguez P.L., Whitehead I.P. Mol. Cell. Biol. 26:8964-8975(2006) [PubMed: 17000758] [Abstract] Cited for: INTERACTION WITH CCPG1.
[4]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-192, MASS SPECTROMETRY. Tissue: Brain.
[5]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74, MASS SPECTROMETRY. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17031 mRNA. Translation: AAA40135.1.
PIR	A43610.
RefSeq	NP_001020566.1. NP_033297.2.
UniGene	Mm.22845
3D structure databases
HSSP	HSSP built from PDB template 1A09 based on UniProtKB P12931.
SMR	P05480. Positions 86-541.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05480.
PTM databases
PhosphoSite	P05480.
Genome annotation databases
Ensembl	ENSMUSG00000027646. Mus musculus. [Contig view]
GeneID	20779.
KEGG	mmu:20779.
Organism-specific databases
MGI	MGI:98397. Src.
Phylogenomic databases
HOGENOM	P05480.
HOVERGEN	P05480.
Gene expression databases
ArrayExpress	P05480.
CleanEx	MM_SRC.
GermOnline	ENSMUSG00000027646. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR000980. SH2. IPR001452. SH3. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. PD000093. SH2. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

SRC_MOUSE

Accession

Primary (citable) accession number: P05480

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 10, 2008
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)