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Reviewed, UniProtKB/Swiss-Prot P05696 (KPCA_RAT)

Last modified September 2, 2008. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Protein kinase C alpha type
      Short name=PKC-alpha
      Short name=PKC-A
    EC=2.7.11.13
Gene names
Name: Prkca
Synonyms: Pkca
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length672 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4 By similarity.

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Binds 3 calcium ions per subunit. The ions are bound to the C2 domain By similarity.

Subunit structure

Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

TRIM41Q8WV441EBI-935801,EBI-725997From a different organism.
TRIM41Q8WV44-21EBI-935801,EBI-726015From a different organism.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 672671Protein kinase C alpha type

Regions

Domain172 – 26089C2
Domain339 – 597259Protein kinase
Domain598 – 66871AGC-kinase C-terminal
Zinc finger36 – 8651Phorbol-ester/DAG-type 1
Zinc finger101 – 15151Phorbol-ester/DAG-type 2
Nucleotide binding345 – 3539ATP By similarity

Sites

Active site4631Proton acceptor By similarity
Metal binding1861Calcium 1; via carbonyl oxygen By similarity
Metal binding1871Calcium 1 By similarity
Metal binding1871Calcium 2 By similarity
Metal binding1931Calcium 2 By similarity
Metal binding2461Calcium 1 By similarity
Metal binding2461Calcium 2 By similarity
Metal binding2471Calcium 2; via carbonyl oxygen By similarity
Metal binding2481Calcium 1 By similarity
Metal binding2481Calcium 2 By similarity
Metal binding2481Calcium 3 By similarity
Metal binding2521Calcium 3; via carbonyl oxygen By similarity
Metal binding2541Calcium 1 By similarity
Metal binding2541Calcium 3 By similarity
Binding site3681ATP By similarity

Amino acid modifications

Modified residue2261Phosphoserine By similarity
Modified residue3191Phosphoserine By similarity
Modified residue4941Phosphothreonine By similarity
Modified residue4951Phosphothreonine By similarity
Modified residue4971Phosphothreonine By similarity
Modified residue5011Phosphothreonine By similarity
Modified residue6311Phosphothreonine; by autocatalysis Potential
Modified residue6381Phosphothreonine; by autocatalysis By similarity
Modified residue6571Phosphoserine By similarity
Modified residue6581Phosphotyrosine By similarity

Secondary structure

1 ........................ 672
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P05696-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 94889E7339C17719

FASTA67276,792
        10         20         30         40         50         60 
MADVYPANDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF 

        70         80         90        100        110        120 
GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS 

       130        140        150        160        170        180 
LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVTD EKLHVTVRDA 

       190        200        210        220        230        240 
KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL 

       250        260        270        280        290        300 
SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNVE 

       310        320        330        340        350        360 
LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG 

       370        380        390        400        410        420 
TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV 

       430        440        450        460        470        480 
NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA 

       490        500        510        520        530        540 
DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG 

       550        560        570        580        590        600 
EDEDELFQSI MEHNVSYPKS LSKEAVSICK GLMTKHPAKR LGCGPEGERD VREHAFFRRI 

       610        620        630        640        650        660 
DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN 

       670 
PQFVHPILQS AV

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References

[1]"Nucleotide sequences of cDNAs for alpha and gamma subspecies of rat brain protein kinase C."
Ono Y., Fujii T., Igarashi K., Kikkawa U., Ogita K., Nishizuka Y.
Nucleic Acids Res. 16:5199-5200(1988) [PubMed: 3387228] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The common structure and activities of four subspecies of rat brain protein kinase C family."
Kikkawa U., Ogita K., Ono Y., Asaoka Y., Shearman M.S., Fujii T., Ase K., Sekiguchi K., Igarashi K., Nishizuka Y.
FEBS Lett. 223:212-216(1987) [PubMed: 3666147] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Targeting of protein kinase Calpha to caveolae."
Mineo C., Ying Y.-S., Chapline C., Jaken S., Anderson R.G.W.
J. Cell Biol. 141:601-610(1998) [PubMed: 9566962] [Abstract]
Cited for: INTERACTION WITH SDPR.
[4]"The molecular heterogeneity of protein kinase C and its implications for cellular regulation."
Nishizuka Y.
Nature 334:661-665(1988) [PubMed: 3045562] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X07286 mRNA. Translation: CAA30266.1.
PIRKIRTC. S02248.
UniGeneRn.207908

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1DSYX-ray2.60A155-293[»]
SMRP05696. Positions 94-158, 95-159, 336-666.
ModBaseSearch...

Protein-protein interaction databases

IntActP05696.

PTM databases

PhosphoSiteP05696.

Genome annotation databases

EnsemblENSRNOG00000003491. Rattus norvegicus. [Contig view]

Organism-specific databases

RGD3395. Prkca.

Phylogenomic databases

HOVERGENP05696.

Gene expression databases

ArrayExpressP05696.
GermOnlineENSRNOG00000003491. Rattus norvegicus.

Family and domain databases

InterProIPR000008. C2_Ca-dep.
IPR002219. DAG_PE_bd.
IPR015745. PKC.
IPR000961. Pkinase_C.
IPR014375. Prot_kin_PKC_alpha.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_bd_CS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PANTHERPTHR22985:SF86. PKC. 1 hit.
PfamPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFPIRSF000550. PKC_alpha. 1 hit.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameKPCA_RAT
AccessionPrimary (citable) accession number: P05696
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: September 2, 2008
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents