Reviewed, UniProtKB/Swiss-Prot P06126 (CD1A_HUMAN)

Names and origin

Protein names

Recommended name:
    T-cell surface glycoprotein CD1a
Alternative name(s):
    T-cell surface antigen T6/Leu-6
      Short name=hTa1 thymocyte antigen
    CD_antigen=CD1a

Gene names

Name:

CD1A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Subunit structure	Heterodimer with B2M (beta-2-microglobulin). Interacts with CD74.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Endosome membrane. Note= Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
Tissue specificity	Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
Miscellaneous	During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.
Sequence similarities	Contains 1 Ig-like (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Immune response
Cellular component	Cell membrane Endosome Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Signal Transmembrane
PTM	Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	immune response Ref.2 Non-traceable author statement. Source: UniProtKB
Cellular component	integral to plasma membrane Ref.2 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 16

16

Chain

17 – 327

311

T-cell surface glycoprotein CD1a

Regions

Topological domain

17 – 300

284

Extracellular Potential

Transmembrane

301 – 321

21

Potential

Topological domain

322 – 327

6

Cytoplasmic Potential

Domain

184 – 291

108

Ig-like

Region

90 – 94

5

Glycolipid binding

Sites

Binding site

171

1

Glycolipid

Amino acid modifications

Glycosylation

37

1

N-linked (GlcNAc...)

Glycosylation

60

1

N-linked (GlcNAc...) Potential

Glycosylation

74

1

N-linked (GlcNAc...)

Glycosylation

145

1

N-linked (GlcNAc...)

Disulfide bond

119 ↔ 183

By similarity

Disulfide bond

223 ↔ 278

Natural variations

Natural variant

30

1

I → T: dbSNP rs2269714.

Natural variant

68

1

W → C: dbSNP rs2269715.

Experimental info

Sequence conflict

68 – 71

4

WPWS → CPV in AAA51933. Ref.4

Secondary structure

1

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327

Helix Strand Turn

Details...

Beta strand

25 – 37

13

Beta strand

40 – 49

10

Beta strand

52 – 58

7

Turn

59 – 62

4

Beta strand

63 – 68

6

Turn

69 – 74

6

Helix

77 – 105

29

Beta strand

109 – 122

14

Beta strand

125 – 135

11

Beta strand

138 – 144

7

Beta strand

147 – 150

4

Helix

152 – 154

3

Helix

156 – 165

10

Helix

169 – 180

12

Helix

182 – 193

12

Helix

195 – 198

4

Beta strand

205 – 211

7

Beta strand

218 – 231

14

Beta strand

234 – 239

6

Beta strand

260 – 269

10

Helix

270 – 272

3

Beta strand

276 – 281

6

Helix

283 – 285

3

Beta strand

290 – 293

4

Sequences

	Sequence		Length	Mass (Da)
	P06126-1 [UniParc]. Last modified December 1, 1992. Version 3. Checksum: AF9E54EA8622A6D6	FASTA	327	37,172
	10 20 30 40 50 60 MLFLLLPLLA VLPGDGNADG LKEPLSFHVI WIASFYNHSW KQNLVSGWLS DLQTHTWDSN 70 80 90 100 110 120 SSTIVFLWPW SRGNFSNEEW KELETLFRIR TIRSFEGIRR YAHELQFEYP FEIQVTGGCE 130 140 150 160 170 180 LHSGKVSGSF LQLAYQGSDF VSFQNNSWLP YPVAGNMAKH FCKVLNQNQH ENDITHNLLS 190 200 210 220 230 240 DTCPRFILGL LDAGKAHLQR QVKPEAWLSH GPSPGPGHLQ LVCHVSGFYP KPVWVMWMRG 250 260 270 280 290 300 EQEQQGTQRG DILPSADGTW YLRATLEVAA GEAADLSCRV KHSSLEGQDI VLYWEHHSSV 310 320 GFIILAVIVP LLLLIGLALW FRKRCFC

References

[1]	"Expression of cDNA clones encoding the thymocyte antigens CD1a, b, c demonstrates a hierarchy of exclusion in fibroblasts." Aruffo A., Seed B. J. Immunol. 143:1723-1730(1989) [PubMed: 2701945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c." Martin L.H., Calabi F., Lefebvre F.-A., Bilsland C.A.G., Milstein C. Proc. Natl. Acad. Sci. U.S.A. 84:9189-9193(1987) [PubMed: 2447586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Polymorphism of human CD1 genes." Han M., Hannick L.I., DiBrino M., Robinson M.A. Tissue Antigens 54:122-127(1999) [PubMed: 10488738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-108, VARIANTS THR-30 AND CYS-68.
[4]	"Molecular cloning of CD1a (T6), a human epidermal dendritic cell marker related to class I MHC molecules." Longley J., Kraus J., Alonso M., Edelson R. J. Invest. Dermatol. 92:628-631(1989) [PubMed: 2784820] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-327, VARIANT THR-30.
[5]	"A novel family of human major histocompatibility complex-related genes not mapping to chromosome 6." Calabi F., Milstein C. Nature 323:540-543(1986) [PubMed: 3093894] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 99-327. Tissue: T-cell.
[6]	"Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens." Martin L.H., Calabi F., Milstein C. Proc. Natl. Acad. Sci. U.S.A. 83:9154-9158(1986) [PubMed: 3097645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-294.
[7]	"CD1a molecules traffic through the early recycling endosomal pathway in human Langerhans cells." Salamero J., Bausinger H., Mommaas A.M., Lipsker D., Proamer F., Cazenave J.-P., Goud B., de la Salle H., Hanau D. J. Invest. Dermatol. 116:401-408(2001) [PubMed: 11231314] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]	"CD1a-, b-, and c-restricted TCRs recognize both self and foreign antigens." Vincent M.S., Xiong X., Grant E.P., Peng W., Brenner M.B. J. Immunol. 175:6344-6351(2005) [PubMed: 16272286] [Abstract] Cited for: FUNCTION.
[9]	"Regulation of CD1a surface expression and antigen presentation by invariant chain and lipid rafts." Sloma I., Zilber M.-T., Vasselon T., Setterblad N., Cavallari M., Mori L., De Libero G., Charron D., Mooney N., Gelin C. J. Immunol. 180:980-987(2008) [PubMed: 18178838] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CD47, TISSUE SPECIFICITY.
[10]	"Crystal structure of CD1a in complex with a sulfatide self antigen at a resolution of 2.15 A." Zajonc D.M., Elsliger M.-A., Teyton L., Wilson I.A. Nat. Immunol. 4:808-815(2003) [PubMed: 12833155] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 18-300 IN COMPLEX WITH B2M AND SULFATIDE SELF-ANTIGEN, MASS SPECTROMETRY, GLYOSYLATION AT ASN-37; ASN-74 AND ASN-145, DISULFIDE BOND.
[11]	"Molecular mechanism of lipopeptide presentation by CD1a." Zajonc D.M., Crispin M.D., Bowden T.A., Young D.C., Cheng T.-Y., Hu J., Costello C.E., Rudd P.M., Dwek R.A., Miller M.J., Brenner M.B., Moody D.B., Wilson I.A. Immunity 22:209-219(2005) [PubMed: 15723809] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 18-294 IN COMPLEX WITH B2M AND LIPOPEPTIDE, GLYOSYLATION AT ASN-37; ASN-74 AND ASN-145, DISULFIDE BOND.
[12]	"Structural characterization of two CD1A allelic variants." Oteo M., Arribas P., Setien F., Parra J.F., Mirones I., Gomez del Moral M., Martinez-Naves E. Hum. Immunol. 62:1137-1141(2001) [PubMed: 11600221] [Abstract] Cited for: VARIANTS THR-30 AND CYS-68, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M28825 mRNA. Translation: AAA51931.1.
M22167 , M22080, M22163, M22164, M22165, M22166 Genomic DNA. Translation: AAA51932.1.
AF142665 Genomic DNA. Translation: AAD37578.1.
M27735 mRNA. Translation: AAA51933.1.
X04450 mRNA. Translation: CAA28049.1.
M14663 Genomic DNA. Translation: AAA51934.1.

PIR

HLHUCD. A39957.

RefSeq

NP_001754.2.

UniGene

Hs.1309

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ONQ	X-ray	2.15	A/C	18-297	[»]
1XZ0	X-ray	2.80	A/C	18-294	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P06126.

Genome annotation databases

Ensembl

ENSG00000158477. Homo sapiens. [Contig view]

GeneID

909.

KEGG

hsa:909.

Organism-specific databases

H-InvDB

HIX0001190.

HGNC

HGNC:1634. CD1A.

HPA

CAB000009.
HPA010734.

MIM

188370. gene.

PharmGKB

PA26193.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P06126.

HOVERGEN

P06126.

Gene expression databases

ArrayExpress

P06126.

CleanEx

HS_CD1A.

GermOnline

ENSG00000158477. Homo sapiens.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
G3DSA:3.30.500.10. MHC_I-like_Ag-recog. 1 hit.

Pfam

PF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProDom

P06126.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

DrugBank

DB00098. Antithymocyte globulin.

SOURCE

Search...

ProtoNet

Search...

Entry information

Entry name

CD1A_HUMAN

Accession

Primary (citable) accession number: P06126
Secondary accession number(s): Q13962, Q9UMM4, Q9Y5M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	December 1, 1992
Last modified:	September 23, 2008
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)