Reviewed, UniProtKB/Swiss-Prot P06199 (ACM2_PIG)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M2

Gene names

Name:

CHRM2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 466	466	Muscarinic acetylcholine receptor M2
Regions
	Topological domain	1 – 22	22	Extracellular Potential
	Transmembrane	23 – 45	23	1 Potential
	Topological domain	46 – 59	14	Cytoplasmic Potential
	Transmembrane	60 – 80	21	2 Potential
	Topological domain	81 – 97	17	Extracellular Potential
	Transmembrane	98 – 119	22	3 Potential
	Topological domain	120 – 139	20	Cytoplasmic Potential
	Transmembrane	140 – 162	23	4 Potential
	Topological domain	163 – 184	22	Extracellular Potential
	Transmembrane	185 – 207	23	5 Potential
	Topological domain	208 – 388	181	Cytoplasmic Potential
	Transmembrane	389 – 409	21	6 Potential
	Topological domain	410 – 423	14	Extracellular Potential
	Transmembrane	424 – 443	20	7 Potential
	Topological domain	444 – 466	23	Cytoplasmic Potential
Amino acid modifications
	Modified residue	232	1	Phosphoserine By similarity
	Modified residue	234	1	Phosphoserine By similarity
	Modified residue	446	1	Phosphothreonine Potential
	Modified residue	450	1	Phosphothreonine Potential
	Modified residue	465	1	Phosphothreonine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	6	1	N-linked (GlcNAc...) Potential
	Disulfide bond	96 ↔ 176		By similarity
Experimental info
	Sequence conflict	330	1	K → N in AAA30986. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P06199-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 53D089F179789CD9	FASTA	466	51,673
	10 20 30 40 50 60 MNNSTNSSNS GLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV NRHLQTVNNY 70 80 90 100 110 120 FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL ALDYVVSNAS VMNLLIISFD 130 140 150 160 170 180 RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL SFILWAPAIL FWQFIVGVRT VEDGECYIQF 190 200 210 220 230 240 FSNAAVTFGT AIAAFYLPVI IMTVLYWHIS RASKSRIKKD KKEPVANQEP VSPSLVQGRI 250 260 270 280 290 300 VKPNNNNMPG SDEALEHNKI QNGKAPRDAV TENCVQGEEK ESSNDSTSVS AVASNMRDDE 310 320 330 340 350 360 ITQDENTVST SLGHSKDENS KQTCIKIVTK TQKSDSCTPA NTTVELVGSS GQNGDEKQNI 370 380 390 400 410 420 VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW APYNVMVLIN TFCAPCIPNT 430 440 450 460 VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR

References

[1]	"Primary structure of porcine cardiac muscarinic acetylcholine receptor deduced from the cDNA sequence." Kubo T., Maeda A., Sugimoto K., Akiba I., Mikami A., Takahashi H., Haga T., Haga K., Ichiyama A., Kangawa K., Matsuo H., Hirose T., Numa S. FEBS Lett. 209:367-372(1986) [PubMed: 3792556] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Primary structure and biochemical properties of an M2 muscarinic receptor." Peralta E.G., Winslow J.W., Peterson G.L., Smith D.H., Ashkenazi A., Ramachandran J., Schimerlik M.I., Capon D.J. Science 236:600-606(1987) [PubMed: 3107123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04708 mRNA. Translation: CAA28413.1. M16331 Genomic DNA. Translation: AAA30986.1.
PIR	A27386.
RefSeq	NP_999426.1.
UniGene	Ssc.16247
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
GeneID	397498.
KEGG	ssc:397498.
Phylogenomic databases
HOVERGEN	P06199.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001065. Musac_M2_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF32. Musac_M2_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00539. MUSCRINICM2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P06199. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM2_PIG

Accession

Primary (citable) accession number: P06199

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	July 22, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)