Reviewed, UniProtKB/Swiss-Prot P06530 (MTBR_BACSU)

Names and origin

Protein names

Recommended name:
    Modification methylase BsuRI
      Short name(s)=M.BsuRI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase BsuRI

Gene names

Name:	hsdRM
Synonyms:	hsdM

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BsuRI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 436	436	Modification methylase BsuRI
Sites
	Active site	157	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P06530-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 07EE0B2CA9140B19	FASTA	436	49,635
	10 20 30 40 50 60 MTLKIDIKGR GKYKPASDYS IDDVKNVLME KIFEESSRII NSDDDLEIIE KVDFRTDKIN 70 80 90 100 110 120 VLSLFSGCGG LDLGFELAGL AAVIGEQAAM EAFKDKDRFN ELRNKSIFHT IYTNDLFKEA 130 140 150 160 170 180 NQTYKTNFPG HVIQHEKDIR QVKYFPKCNL ILGGFPCPGF SEAGPRLIDD DRNFLYLHFI 190 200 210 220 230 240 RSLIQAQPEI FVAENVKGMM TLGKGEVLNQ IIEDFASAGY RVQFKLLNAR DYGVPQLRER 250 260 270 280 290 300 VIIEGVRKDI SFNYKYPSPT HGEETGLKPF KTLRDSIGDL VTDPGPYFTG SYSSIYMSRN 310 320 330 340 350 360 RKKSWDEQSF TIQASGRQAP LHPGGLSMKK IGKDKWVFPD GEENHRRLSV KEIARVQTFP 370 380 390 400 410 420 DWFQFSQGTN SQTSINNRLD KQYKQIGNAV PVLLAKAVAS PIANWAINYL ESSPNNKIKN 430 RERKLSIRTF LRIKTS

References

[1]	"Nucleotide sequence of the BsuRI restriction-modification system." Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J. Nucleic Acids Res. 13:6403-6421(1985) [PubMed: 2997708] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02988 Genomic DNA. Translation: CAA26731.1.
PIR	XYBSR1. B23488.
3D structure databases
HSSP	HSSP built from PDB template 1DCT based on UniProtKB P20589.
ModBase	Search...
Protein family/group databases
REBASE	3340. M.BsuRI.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProDom	P06530. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTBR_BACSU

Accession

Primary (citable) accession number: P06530

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	July 22, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)